##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527542
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745383305973743	33.0	33.0	33.0	27.0	33.0
2	31.244050710654317	33.0	33.0	33.0	27.0	33.0
3	31.69769989877583	33.0	33.0	33.0	27.0	33.0
4	31.815586247161363	33.0	33.0	33.0	27.0	33.0
5	31.934162588002472	33.0	33.0	33.0	33.0	33.0
6	34.754358894647254	37.0	37.0	37.0	27.0	37.0
7	34.94705634812015	37.0	37.0	37.0	27.0	37.0
8	35.136565429861506	37.0	37.0	37.0	33.0	37.0
9	35.218077423219384	37.0	37.0	37.0	33.0	37.0
10	35.222359546728036	37.0	37.0	37.0	33.0	37.0
11	35.2699102630691	37.0	37.0	37.0	33.0	37.0
12	35.1906502231102	37.0	37.0	37.0	33.0	37.0
13	35.223032478930584	37.0	37.0	37.0	33.0	37.0
14	35.196632685170094	37.0	37.0	37.0	33.0	37.0
15	35.21282476087212	37.0	37.0	37.0	33.0	37.0
16	35.231767328478114	37.0	37.0	37.0	33.0	37.0
17	35.19148996667564	37.0	37.0	37.0	33.0	37.0
18	35.1931523935535	37.0	37.0	37.0	33.0	37.0
19	35.20146263235913	37.0	37.0	37.0	33.0	37.0
20	35.18381474839918	37.0	37.0	37.0	33.0	37.0
21	35.197847754302025	37.0	37.0	37.0	33.0	37.0
22	35.18327071588613	37.0	37.0	37.0	33.0	37.0
23	35.17916867282605	37.0	37.0	37.0	33.0	37.0
24	35.16985946142677	37.0	37.0	37.0	33.0	37.0
25	35.171118128983096	37.0	37.0	37.0	33.0	37.0
26	35.144089001444435	37.0	37.0	37.0	33.0	37.0
27	35.07560345906108	37.0	37.0	37.0	33.0	37.0
28	35.050242445151284	37.0	37.0	37.0	27.0	37.0
29	35.08467761808539	37.0	37.0	37.0	33.0	37.0
30	35.033398288667065	37.0	37.0	37.0	27.0	37.0
31	35.00585356237039	37.0	37.0	37.0	27.0	37.0
32	35.03591941494706	37.0	37.0	37.0	27.0	37.0
33	35.02379526179906	37.0	37.0	37.0	27.0	37.0
34	34.954993156942955	37.0	37.0	37.0	27.0	37.0
35	34.93380053152166	37.0	37.0	37.0	27.0	37.0
36	34.91616402106373	37.0	37.0	37.0	27.0	37.0
37	34.912869496646714	37.0	37.0	37.0	27.0	37.0
38	34.766323818767034	37.0	37.0	37.0	27.0	37.0
39	34.822548725978216	37.0	37.0	37.0	27.0	37.0
40	34.821066379548924	37.0	37.0	37.0	27.0	37.0
41	34.82978796001077	37.0	37.0	37.0	27.0	37.0
42	34.69497973621058	37.0	37.0	37.0	27.0	37.0
43	34.45828768135997	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	15.0
16	25.0
17	36.0
18	28.0
19	26.0
20	42.0
21	103.0
22	321.0
23	705.0
24	1498.0
25	2684.0
26	4467.0
27	6886.0
28	9666.0
29	12972.0
30	17179.0
31	21938.0
32	27325.0
33	35625.0
34	50206.0
35	89872.0
36	245923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11599834705104	19.867612436545336	13.87491422483897	23.141474991564653
2	16.892493867786833	23.390933802427107	38.39770103612604	21.31887129366003
3	17.78739891800084	28.694018675290305	30.133335355289248	23.385247051419604
4	11.766266951256961	18.580511125180553	40.8350425179417	28.818179405620786
5	13.775016965473839	36.779820374491514	34.703777140019184	14.741385520015468
6	26.593901528219554	42.501829238240745	16.62142540309587	14.28284383044383
7	26.008355732813694	32.91169233918816	22.595546894844393	18.48440503315376
8	24.171535157390313	34.25622983572872	20.05736036182901	21.514874645051957
9	27.313275530668648	14.04741233873322	19.5775881351627	39.06172399543543
10	16.947086677458856	26.371739122193116	32.335055787027386	24.346118413320646
11	34.48388943439574	24.54117397287799	22.639713994336	18.33522259839027
12	24.426112044159517	26.79653942245357	27.725564978712598	21.05178355467432
13	29.151991689761193	22.87647239461503	24.90702162102733	23.06451429459645
14	23.257295153750793	21.05936588935099	28.177282567075228	27.50605638982299
15	25.65937877931994	26.800141031424985	26.409271678842632	21.131208510412442
16	22.135867855071258	27.84688233353932	25.538440541227047	24.478809270162376
17	22.576211941418883	25.742784460763314	25.056014497423902	26.624989100393904
18	24.144238752554298	25.141315762536443	28.036440700456076	22.678004784453183
19	27.596286172475367	23.840945365487485	27.848209242107735	20.71455921992941
20	26.210424951946955	23.99448764269006	27.90128558484443	21.893801820518554
21	23.314352222192735	26.54897619526028	28.02051779763507	22.116153784911912
22	23.18829590819309	25.937840020320657	27.864700820029498	23.00916325145676
23	22.87855753665111	26.1340329300795	29.32543759549003	21.661971937779363
24	23.49708648790049	26.62233528325707	26.289091674217406	23.591486554625035
25	23.480784468345647	26.365862812818698	28.897983478092737	21.255369240742915
26	23.336340992755076	26.66441724071259	27.936543441090944	22.06269832544139
27	23.426002100306707	26.19582895769436	27.3794314007226	22.998737541276334
28	22.306849502030172	25.96627377535817	28.822728806426788	22.904147916184872
29	24.75158376015559	25.88571146941855	26.754267906631128	22.608436863794733
30	24.402796365028756	25.180364786121295	28.516402485489305	21.900436363360644
31	23.545802988198098	25.535218048989467	28.09387688563185	22.825102077180585
32	21.388060097584646	25.44953766714309	28.885472625876236	24.276929609396028
33	21.641689192519266	25.480056564216685	29.55745703659614	23.320797206667905
34	22.6850184440291	25.496358583771528	28.981389159536114	22.837233812663257
35	22.79382494663932	26.493435593753674	27.7685947280027	22.944144731604307
36	23.651386998570732	26.101997566070565	27.651068540514313	22.595546894844393
37	23.22165818077044	25.485174640123443	27.92687596437819	23.366291214727926
38	22.978454796016244	25.953194248040912	27.312706855567896	23.755644100374944
39	22.158804417468183	25.796050361866925	28.42143374366401	23.62371147700088
40	22.3625796619037	26.883925829602195	28.82955290763579	21.92394160085832
41	23.157208336018744	24.979622475556447	28.181642409514314	23.681526778910495
42	21.7404491016829	27.330146225324242	27.999666377274224	22.929738295718636
43	23.396051878333857	25.266992959802252	27.781674255319956	23.555280906543935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	77.5
2	87.0
3	280.5
4	474.0
5	474.0
6	602.0
7	730.0
8	746.0
9	762.0
10	943.5
11	1125.0
12	1125.0
13	1843.5
14	2562.0
15	4440.5
16	6319.0
17	6724.5
18	7130.0
19	7130.0
20	5281.5
21	3433.0
22	2474.5
23	1516.0
24	1739.5
25	1963.0
26	1963.0
27	2746.0
28	3529.0
29	5140.5
30	6752.0
31	9641.5
32	12531.0
33	12531.0
34	17211.0
35	21891.0
36	24833.0
37	27775.0
38	31363.5
39	34952.0
40	34952.0
41	39497.0
42	44042.0
43	46911.5
44	49781.0
45	49142.5
46	48504.0
47	48504.0
48	48503.5
49	48503.0
50	48274.0
51	48045.0
52	45604.0
53	43163.0
54	43163.0
55	40150.5
56	37138.0
57	30668.5
58	24199.0
59	22318.5
60	20438.0
61	20438.0
62	16871.5
63	13305.0
64	9673.0
65	6041.0
66	5357.5
67	4674.0
68	4674.0
69	3715.0
70	2756.0
71	2087.5
72	1419.0
73	1290.0
74	1161.0
75	1161.0
76	732.0
77	303.0
78	231.5
79	160.0
80	136.5
81	113.0
82	113.0
83	105.0
84	97.0
85	71.5
86	46.0
87	41.0
88	36.0
89	36.0
90	22.0
91	8.0
92	8.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	527542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.475255174086968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92869680671944	18.828494845544277
2	8.29998473592322	4.062884887054953
3	3.391996443921572	2.490599355437282
4	2.009278874048994	1.9671045263300515
5	1.3117134463665767	1.6052260657551503
6	0.9868882318821045	1.449260478217081
7	0.7555214999739056	1.2944107050959197
8	0.5654194419714974	1.1071028098113807
9	0.48893323475773454	1.077008911540859
>10	4.1052896527112495	20.85991070463423
>50	0.5978349278250991	10.22436526885717
>100	0.5090093248536696	24.346103409224572
>500	0.040937017021615306	6.959729446286826
>1k	0.007723965475776473	2.757992472956092
>5k	7.723965475776474E-4	0.969806113254147
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5130	0.9724344222829652	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3863	0.7322639713994336	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1842	0.349166511860667	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1203	0.22803871540085907	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1167	0.22121461419185579	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1140	0.2160965382851034	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1126	0.21344272114826876	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1094	0.20737685340693254	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1078	0.20434391953626443	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1071	0.2030170109678471	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1005	0.1905061587513411	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	975	0.1848194077438384	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	965	0.18292382407467084	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	943	0.17875354000250215	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	939	0.17799530653483514	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	935	0.1772370730671681	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	908	0.17211899716041568	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	905	0.1715503220596654	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	901	0.17079208859199835	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	900	0.1706025302250816	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	896	0.16984429675741458	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	844	0.1599872616777432	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	836	0.15847079474240913	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	835	0.15828123637549238	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	821	0.15562741923865778	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	785	0.14880331802965452	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	761	0.14425391722365233	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	758	0.14368524212290207	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	756	0.14330612538906853	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	733	0.13894628294998315	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	726	0.13761937438156582	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	714	0.13534467397856473	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	704	0.13344909030939717	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	688	0.13041615643872906	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	687	0.1302265980718123	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	682	0.1292788062372285	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	681	0.12908924787031173	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	664	0.12586675563272687	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	659	0.12491896379814309	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	652	0.12359205522972577	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	644	0.12207558829439172	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	640	0.1213173548267247	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	634	0.12018000462522414	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	624	0.1182844209560566	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	605	0.11468281198463819	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	592	0.11221855321472034	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	587	0.11127076138013656	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	581	0.11013341117863601	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	566	0.10729003567488465	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	564	0.10691091894105113	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	561	0.10634224384030086	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	554	0.10501533527188357	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	553	0.1048257769049668	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	547	0.10368842670346627	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	546	0.1034988683365495	No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC	542	0.10274063486888248	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.10217195976813222	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	539	0.10217195976813222	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	536	0.10160328466738194	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	531	0.10065549283279815	No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA	528	0.10008681773204789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0015164669353340586	0.0	0.0
2	3.7911673383351466E-4	0.0	0.0020851420360843307	0.0	0.0
3	3.7911673383351466E-4	0.0	0.002653817136834603	0.0	0.0
4	3.7911673383351466E-4	0.0	0.003412050604501632	1.8955836691675733E-4	0.0
5	3.7911673383351466E-4	0.0	0.004359842439085419	3.7911673383351466E-4	0.0
6	3.7911673383351466E-4	0.0	0.00928835997892111	3.7911673383351466E-4	0.0
7	3.7911673383351466E-4	0.0	0.020851420360843306	3.7911673383351466E-4	0.0
8	3.7911673383351466E-4	0.0	0.024263470965344938	3.7911673383351466E-4	0.0
9	3.7911673383351466E-4	0.0	0.030518897073597933	7.582334676670293E-4	0.0
10	0.0020851420360843307	0.0	0.03563697298035038	7.582334676670293E-4	0.0
11	0.002274700403001088	0.0	0.05326590110360881	9.477918345837866E-4	0.0
12	0.002274700403001088	0.0	0.057246626808860716	0.001137350201500544	0.0
13	0.002274700403001088	0.0	0.06388116965094723	0.001137350201500544	0.0
14	0.002274700403001088	0.0	0.06767233698928236	0.0013269085684173014	0.0
15	0.002274700403001088	0.0	0.07430687983136888	0.001706025302250816	0.0
16	0.002274700403001088	0.0	0.08094142267345539	0.001706025302250816	0.0
17	0.002274700403001088	0.0	0.08283700634262296	0.001706025302250816	0.0
18	0.0024642587699178456	0.0	0.08624905694712459	0.001706025302250816	0.0
19	0.0024642587699178456	0.0	0.08833419898320892	0.0020851420360843307	0.0
20	0.0024642587699178456	0.0	0.09136713285387704	0.0020851420360843307	0.0
21	0.0024642587699178456	0.0	0.09610609202679597	0.0024642587699178456	0.0
22	0.0024642587699178456	0.0	0.096864325494463	0.003412050604501632	0.0
23	0.0024642587699178456	0.0	0.09781211732904678	0.0036016089714183894	0.0
24	0.0024642587699178456	0.0	0.0981912340628803	0.004928517539835691	0.0
25	0.0024642587699178456	0.0	0.09838079242979705	0.004928517539835691	0.0
26	0.0024642587699178456	0.0	0.09838079242979705	0.005876309374419478	0.0
27	0.0024642587699178456	0.0	0.09875990916363057	0.008151009777420566	0.0
28	0.0024642587699178456	0.0	0.09875990916363057	0.02502170443301197	0.0
29	0.0024642587699178456	0.0	0.09894946753054733	0.06767233698928236	0.0
30	0.0024642587699178456	0.0	0.09894946753054733	0.11809486258913982	0.0
31	0.0024642587699178456	0.0	0.0991390258974641	0.24244515128653263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	895	0.0	30.178772	1
CCATAGC	25	0.005493682	29.599998	9
AGCCTCG	25	0.005493682	29.599998	10
GTGACAT	25	0.005493682	29.599998	16
TCCGGGT	25	0.005493682	29.599998	13
CGCCAGG	40	5.9341306E-5	27.75	14
ACTAGTG	50	9.078918E-6	25.899998	8
GGTAGTT	50	9.078918E-6	25.899998	6
AGAGGTG	215	0.0	24.953487	20
TAGACAT	45	1.3218864E-4	24.666668	20
TATTGCG	45	1.3218864E-4	24.666668	9
TTGTTAC	60	1.3351146E-6	24.666668	15
CTTAGGA	95	2.8012437E-10	23.368422	2
GTATCAA	2535	0.0	23.207102	1
TACATAC	40	0.0019298097	23.125	3
GAATTAG	40	0.0019298097	23.125	1
GCTAGAG	225	0.0	23.022223	17
GTAAACA	225	0.0	23.022223	36
CGTTATT	65	2.6782782E-6	22.769234	10
CGCCGCC	195	0.0	22.769232	33
>>END_MODULE