FastQCFastQC Report
Fri 10 Feb 2017
ERR1633084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468297
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19220.4104232997435388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.3036534506947514No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8030.1714723775723526No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7930.16933698059137683No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7510.1603683132712787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7000.14947778866830239No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6110.13047275553761822No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5530.11808745304795888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT251.2325088E-436.9999966
GGATCGC250.005493059329.5999987
CTCGCAC250.005493059329.5999988
CGTGTCT405.9327285E-527.7510
CCGTGTC451.3215769E-424.6666669
GGTATCA4050.024.6666661
GTATCAA11300.024.2300871
GGACCGT551.8995388E-523.5454546
GCACCGC952.8012437E-1023.36842210
TTAACGG952.8012437E-1023.36842235
CGGATCG400.001929487823.12537
GACGGAT400.001929487823.12535
TAAGGAT652.6772123E-622.769234
GTATTAG1155.456968E-1222.521741
TCTATAC753.7309837E-722.23
CCTTAGC705.091275E-621.1428572
GACCGTG450.003822473820.5555557
GGACGGA450.003822473820.55555534
AACGGCC1101.7462298E-920.18181837
ACCGTGT656.890972E-519.9230778