##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468297
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678932386925393	33.0	33.0	33.0	27.0	33.0
2	31.20079564891511	33.0	33.0	33.0	27.0	33.0
3	31.64771715385749	33.0	33.0	33.0	27.0	33.0
4	31.784312092539565	33.0	33.0	33.0	27.0	33.0
5	31.901485595679667	33.0	33.0	33.0	33.0	33.0
6	34.75163197714271	37.0	37.0	37.0	27.0	37.0
7	34.93329019831432	37.0	37.0	37.0	27.0	37.0
8	35.11926405678447	37.0	37.0	37.0	33.0	37.0
9	35.19159422332409	37.0	37.0	37.0	33.0	37.0
10	35.23170124942077	37.0	37.0	37.0	33.0	37.0
11	35.25438130075572	37.0	37.0	37.0	33.0	37.0
12	35.216159830193234	37.0	37.0	37.0	33.0	37.0
13	35.245583465194095	37.0	37.0	37.0	33.0	37.0
14	35.205438001951755	37.0	37.0	37.0	33.0	37.0
15	35.22608301996382	37.0	37.0	37.0	33.0	37.0
16	35.2473451677034	37.0	37.0	37.0	33.0	37.0
17	35.18543360303397	37.0	37.0	37.0	33.0	37.0
18	35.218158561767424	37.0	37.0	37.0	33.0	37.0
19	35.20680465601958	37.0	37.0	37.0	33.0	37.0
20	35.198551346688106	37.0	37.0	37.0	33.0	37.0
21	35.18363346337901	37.0	37.0	37.0	33.0	37.0
22	35.18493392014042	37.0	37.0	37.0	33.0	37.0
23	35.16494233360453	37.0	37.0	37.0	33.0	37.0
24	35.177577477540964	37.0	37.0	37.0	33.0	37.0
25	35.17168591727045	37.0	37.0	37.0	33.0	37.0
26	35.12479687036218	37.0	37.0	37.0	33.0	37.0
27	35.059075757478695	37.0	37.0	37.0	33.0	37.0
28	35.04187513479693	37.0	37.0	37.0	27.0	37.0
29	35.08502937238547	37.0	37.0	37.0	33.0	37.0
30	35.04527682218763	37.0	37.0	37.0	27.0	37.0
31	35.0470983158124	37.0	37.0	37.0	27.0	37.0
32	35.07766865899205	37.0	37.0	37.0	33.0	37.0
33	35.04676305848639	37.0	37.0	37.0	27.0	37.0
34	34.989831239576596	37.0	37.0	37.0	27.0	37.0
35	34.99487718264264	37.0	37.0	37.0	27.0	37.0
36	34.967642329547274	37.0	37.0	37.0	27.0	37.0
37	34.99173174288966	37.0	37.0	37.0	27.0	37.0
38	34.84555100715998	37.0	37.0	37.0	27.0	37.0
39	34.9197325628821	37.0	37.0	37.0	27.0	37.0
40	34.94428535736936	37.0	37.0	37.0	27.0	37.0
41	34.960048857882924	37.0	37.0	37.0	27.0	37.0
42	34.867564814636864	37.0	37.0	37.0	27.0	37.0
43	34.646600341236436	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	32.0
17	19.0
18	19.0
19	16.0
20	25.0
21	83.0
22	267.0
23	607.0
24	1329.0
25	2419.0
26	3960.0
27	6003.0
28	8539.0
29	11305.0
30	15018.0
31	18964.0
32	23837.0
33	31424.0
34	44670.0
35	79767.0
36	219984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7361204534729	20.04838809558891	13.755159652955282	24.460331797982903
2	16.246740850357785	23.3247276835	37.60925224803917	22.81927921810304
3	18.420788516689196	26.747769044004126	30.23316399635274	24.598278442953937
4	12.766897930159708	17.911282797028385	39.72585773558233	29.595961537229577
5	14.223665750581363	36.51550191438339	35.05061958543403	14.210212749601215
6	29.234438828350385	39.97313670597932	17.287746878583462	13.504677587086828
7	26.669613514500412	32.0856208773492	23.009970168504175	18.234795439646206
8	24.73729278641546	34.72326322825045	20.970452511974237	19.568991473359855
9	26.39158482757737	15.245026126582061	20.129319641167893	38.234069404672674
10	15.533304719013788	27.90942500165493	33.25667258171631	23.300597697614975
11	34.41533898359375	23.97388836571663	23.725968776225344	17.884803874464282
12	23.64268829396729	25.37449524553862	30.004676519388333	20.97813994110575
13	29.19941831786238	22.026192779368646	25.617930501369855	23.156458401399114
14	22.636702776229615	21.070602630382	27.821446646038734	28.471247947349653
15	24.29163543648582	28.37750401988482	24.460972417077198	22.869888126552166
16	22.744753863466986	28.11741266760197	25.26025150705642	23.877581961874622
17	22.852164331610066	26.99056368074107	26.038603706622084	24.118668281026785
18	23.769103795241055	26.135977808954575	27.71424971759375	22.380668678210622
19	25.71615876249474	25.806699594488116	27.089432560960248	21.3877090820569
20	25.67152896559235	25.295272017544423	26.811830953433397	22.221368063429832
21	24.000580827978826	26.385605716030643	27.021740476663314	22.59207297932722
22	23.441533898359374	26.37322041354098	27.28204536864426	22.90320031945539
23	23.47634086914928	26.254705881096825	27.457147921084267	22.811805328669628
24	23.155177163210528	26.50518794696528	26.96237644059219	23.377258449232006
25	23.71294285464139	26.822935017734473	27.18851498087752	22.275607146746616
26	23.90000363017487	26.47571946862782	26.854538893052915	22.769738008144405
27	23.765900699769592	26.405464907953714	26.860731544297746	22.967902847978955
28	23.139375225551305	26.61622858997602	27.03732887462444	23.207067309848238
29	23.966414476283212	26.62946805125807	26.5122347570025	22.891882715456216
30	24.130199424724054	26.188935654082773	26.912835230633554	22.768029690559626
31	23.877581961874622	26.144946476274672	26.925434072821307	23.0520374890294
32	22.481032336316485	26.34439255429781	27.30233163996353	23.872243469422184
33	22.751373594108014	26.63480654371051	27.567120865604522	23.046698996576957
34	23.347149351800248	26.009135228284613	27.180827551746006	23.462887868169133
35	23.197458023433846	26.674311387858562	26.954689011460676	23.17354157724692
36	23.478049186734058	26.437068783272156	27.21157726827206	22.873304761721727
37	23.80583262331384	25.791111196526987	27.12060935688249	23.282446823276683
38	23.71849488679193	26.261752691134042	26.61024947842929	23.40950294364474
39	23.34949828847932	26.01660911771803	27.352299929318356	23.28159266448429
40	23.145567876796136	26.575228967941282	27.284394305323328	22.994808849939247
41	23.425731960700155	25.576076720542734	27.344612500186848	23.653578818570267
42	22.78660764429411	26.545760489603822	27.474231096932073	23.19340076916999
43	23.55663179563397	25.87994371093558	27.291014035964356	23.272410457466094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	18.5
2	24.0
3	109.5
4	195.0
5	195.0
6	220.0
7	245.0
8	244.5
9	244.0
10	340.0
11	436.0
12	436.0
13	665.5
14	895.0
15	1692.5
16	2490.0
17	2806.5
18	3123.0
19	3123.0
20	2928.5
21	2734.0
22	3149.5
23	3565.0
24	4784.0
25	6003.0
26	6003.0
27	7690.5
28	9378.0
29	11260.5
30	13143.0
31	15466.0
32	17789.0
33	17789.0
34	20450.5
35	23112.0
36	24674.5
37	26237.0
38	28266.5
39	30296.0
40	30296.0
41	31826.0
42	33356.0
43	34465.5
44	35575.0
45	35922.0
46	36269.0
47	36269.0
48	36613.0
49	36957.0
50	35948.5
51	34940.0
52	34296.0
53	33652.0
54	33652.0
55	31849.0
56	30046.0
57	27040.5
58	24035.0
59	22052.0
60	20069.0
61	20069.0
62	17670.0
63	15271.0
64	12772.5
65	10274.0
66	8562.5
67	6851.0
68	6851.0
69	5826.5
70	4802.0
71	3950.0
72	3098.0
73	2283.0
74	1468.0
75	1468.0
76	1104.5
77	741.0
78	555.0
79	369.0
80	299.5
81	230.0
82	230.0
83	188.0
84	146.0
85	136.5
86	127.0
87	92.5
88	58.0
89	58.0
90	46.5
91	35.0
92	20.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	468297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16347153098243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36503774037504	56.4460601717935
2	5.909029999953253	7.464696963555369
3	1.5282786383075788	2.8959415278644802
4	0.7680881513515965	1.9406045632472595
5	0.4430706164572752	1.399293913440697
6	0.2859690778713626	1.083767982532147
7	0.23599668380974698	1.0434458873456252
8	0.17698203635864082	0.8943039852027445
9	0.13493180155634987	0.7670484905605808
>10	0.9793033088136586	12.453584446747115
>50	0.10793690891320333	4.758174392129988
>100	0.06265107638931473	7.228235535870789
>500	0.002042969882260263	0.905653242927546
>1k	6.80989960753421E-4	0.7191888967821689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.4104232997435388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1422	0.3036534506947514	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	803	0.1714723775723526	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	793	0.16933698059137683	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	751	0.1603683132712787	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	700	0.14947778866830239	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	611	0.13047275553761822	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	553	0.11808745304795888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.1353969809757484E-4	0.0	0.0
2	0.0	0.0	2.1353969809757484E-4	0.0	0.0
3	0.0	0.0	6.406190942927245E-4	0.0	0.0
4	0.0	0.0	8.541587923902994E-4	0.0	0.0
5	0.0	0.0	8.541587923902994E-4	0.0	0.0
6	0.0	0.0	0.0019218572828781735	0.0	0.0
7	0.0	0.0	0.005338492452439371	0.0	0.0
8	0.0	0.0	0.006406190942927245	0.0	0.0
9	0.0	0.0	0.007900968829610269	2.1353969809757484E-4	0.0
10	4.270793961951497E-4	0.0	0.008968667320098142	2.1353969809757484E-4	0.0
11	4.270793961951497E-4	0.0	0.01281238188585449	2.1353969809757484E-4	0.0
12	6.406190942927245E-4	0.0	0.013453000980147215	2.1353969809757484E-4	0.0
13	6.406190942927245E-4	0.0	0.015801937659220538	2.1353969809757484E-4	0.0
14	6.406190942927245E-4	0.0	0.016229017055415688	2.1353969809757484E-4	0.0
15	6.406190942927245E-4	0.0	0.017083175847805988	2.1353969809757484E-4	0.0
16	6.406190942927245E-4	0.0	0.01857795373448901	2.1353969809757484E-4	0.0
17	6.406190942927245E-4	0.0	0.018791493432586584	2.1353969809757484E-4	0.0
18	6.406190942927245E-4	0.0	0.019218572828781734	2.1353969809757484E-4	0.0
19	6.406190942927245E-4	0.0	0.020072731621172034	2.1353969809757484E-4	0.0
20	6.406190942927245E-4	0.0	0.020072731621172034	2.1353969809757484E-4	0.0
21	6.406190942927245E-4	0.0	0.020072731621172034	4.270793961951497E-4	0.0
22	6.406190942927245E-4	0.0	0.02071335071546476	4.270793961951497E-4	0.0
23	6.406190942927245E-4	0.0	0.02071335071546476	0.001281238188585449	0.0
24	6.406190942927245E-4	0.0	0.020926890413562334	0.0021353969809757485	0.0
25	6.406190942927245E-4	0.0	0.020926890413562334	0.002348936679073323	0.0
26	6.406190942927245E-4	0.0	0.020926890413562334	0.002348936679073323	0.0
27	6.406190942927245E-4	0.0	0.020926890413562334	0.004270793961951497	0.0
28	6.406190942927245E-4	0.0	0.020926890413562334	0.01195822309346419	0.0
29	6.406190942927245E-4	0.0	0.020926890413562334	0.02647892256409928	0.0
30	6.406190942927245E-4	0.0	0.020926890413562334	0.05317138482629613	0.0
31	6.406190942927245E-4	0.0	0.020926890413562334	0.12278532640610552	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGT	25	1.2325088E-4	36.999996	6
GGATCGC	25	0.0054930593	29.599998	7
CTCGCAC	25	0.0054930593	29.599998	8
CGTGTCT	40	5.9327285E-5	27.75	10
CCGTGTC	45	1.3215769E-4	24.666666	9
GGTATCA	405	0.0	24.666666	1
GTATCAA	1130	0.0	24.230087	1
GGACCGT	55	1.8995388E-5	23.545454	6
GCACCGC	95	2.8012437E-10	23.368422	10
TTAACGG	95	2.8012437E-10	23.368422	35
CGGATCG	40	0.0019294878	23.125	37
GACGGAT	40	0.0019294878	23.125	35
TAAGGAT	65	2.6772123E-6	22.76923	4
GTATTAG	115	5.456968E-12	22.52174	1
TCTATAC	75	3.7309837E-7	22.2	3
CCTTAGC	70	5.091275E-6	21.142857	2
GACCGTG	45	0.0038224738	20.555555	7
GGACGGA	45	0.0038224738	20.555555	34
AACGGCC	110	1.7462298E-9	20.181818	37
ACCGTGT	65	6.890972E-5	19.923077	8
>>END_MODULE