FastQCFastQC Report
Fri 10 Feb 2017
ERR1633083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413436
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11520.27864046672278175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.20486846815468418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4740.1146489420369779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC250.005492324529.59999824
CGCTTTA250.005492324529.59999823
GTATCAA7800.024.6666641
GGTATCA2800.024.4464281
CAATTAC400.001929107623.1251
AATACGC400.001929107623.1255
CGCGCGA502.698049E-422.19999932
CGCGATA502.698049E-422.19999934
CTTAGGC502.698049E-422.1999993
ATACGCC450.003821726420.5555576
GTATAGA555.13544E-420.1818181
AATATAG656.888474E-519.9230775
GCGATAC759.242256E-619.73333435
GGACTAG701.2168026E-418.519
TAGCACC609.2231855E-418.54
ACGGGAC1156.375558E-817.69565216
TGTGCAA1054.778922E-717.61904710
CGATACA852.7162918E-517.41176436
GGACTGA752.0632346E-417.2666666
TTAACGG752.0632346E-417.26666635
Produced by FastQC (version 0.11.5)