##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413436
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.657323019766057	33.0	33.0	33.0	27.0	33.0
2	31.18929652957169	33.0	33.0	33.0	27.0	33.0
3	31.624074826575335	33.0	33.0	33.0	27.0	33.0
4	31.761750307181764	33.0	33.0	33.0	27.0	33.0
5	31.87536402248474	33.0	33.0	33.0	33.0	33.0
6	34.75559699687497	37.0	37.0	37.0	27.0	37.0
7	34.93490165346027	37.0	37.0	37.0	27.0	37.0
8	35.100494393328106	37.0	37.0	37.0	33.0	37.0
9	35.189103029247576	37.0	37.0	37.0	33.0	37.0
10	35.207558122659854	37.0	37.0	37.0	33.0	37.0
11	35.25142222738223	37.0	37.0	37.0	33.0	37.0
12	35.20672849002022	37.0	37.0	37.0	33.0	37.0
13	35.22994611015974	37.0	37.0	37.0	33.0	37.0
14	35.197967762846005	37.0	37.0	37.0	33.0	37.0
15	35.216405924979924	37.0	37.0	37.0	33.0	37.0
16	35.22243829758415	37.0	37.0	37.0	33.0	37.0
17	35.19331408005108	37.0	37.0	37.0	33.0	37.0
18	35.193725268239824	37.0	37.0	37.0	33.0	37.0
19	35.18508789752223	37.0	37.0	37.0	33.0	37.0
20	35.19535792722453	37.0	37.0	37.0	33.0	37.0
21	35.20282703973529	37.0	37.0	37.0	33.0	37.0
22	35.21732747027351	37.0	37.0	37.0	33.0	37.0
23	35.20398320417187	37.0	37.0	37.0	33.0	37.0
24	35.19157983339622	37.0	37.0	37.0	33.0	37.0
25	35.184606565466	37.0	37.0	37.0	33.0	37.0
26	35.15497440958214	37.0	37.0	37.0	33.0	37.0
27	35.08754196538279	37.0	37.0	37.0	33.0	37.0
28	35.06244981085343	37.0	37.0	37.0	33.0	37.0
29	35.08523931152585	37.0	37.0	37.0	33.0	37.0
30	35.05865478574677	37.0	37.0	37.0	27.0	37.0
31	35.058961967511294	37.0	37.0	37.0	27.0	37.0
32	35.076432628024655	37.0	37.0	37.0	33.0	37.0
33	35.0546952853646	37.0	37.0	37.0	27.0	37.0
34	34.98737410385162	37.0	37.0	37.0	27.0	37.0
35	34.98308081541037	37.0	37.0	37.0	27.0	37.0
36	34.95727996594394	37.0	37.0	37.0	27.0	37.0
37	35.005983997523195	37.0	37.0	37.0	27.0	37.0
38	34.85730560473689	37.0	37.0	37.0	27.0	37.0
39	34.93555471705415	37.0	37.0	37.0	27.0	37.0
40	34.95538608152169	37.0	37.0	37.0	27.0	37.0
41	34.974467632233285	37.0	37.0	37.0	27.0	37.0
42	34.8936159405567	37.0	37.0	37.0	27.0	37.0
43	34.67218384465794	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	22.0
17	26.0
18	25.0
19	17.0
20	26.0
21	88.0
22	233.0
23	564.0
24	1191.0
25	2147.0
26	3521.0
27	5292.0
28	7357.0
29	9955.0
30	13033.0
31	16791.0
32	21202.0
33	27787.0
34	39186.0
35	70813.0
36	194148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.970791126075135	20.093073655898376	13.61516655540398	25.320968662622512
2	16.20178213798508	22.848276395862964	37.77077951605569	23.179161950096265
3	17.86056366644414	26.690225331127433	29.977553962402887	25.47165704002554
4	13.005398659042754	17.91426000638551	38.67297477723275	30.40736655733898
5	14.397149740225816	36.46997358720576	35.02694492013274	14.105931752435685
6	29.92071324219468	39.443589818012946	17.224673226327656	13.41102371346472
7	27.078193480974083	31.70430247970665	23.380644162578974	17.836859876740295
8	24.705153881132752	35.67734788455771	20.527481883532158	19.090016350777386
9	26.413277992240637	15.337803190820345	19.919890865817198	38.329027951121816
10	15.143335365086735	27.81131783395737	34.00066757611819	23.0446792248377
11	34.35211253978851	23.595913273154736	23.727977244361885	18.32399694269488
12	23.359359126926538	25.23195851353051	30.201046836753452	21.2076355227895
13	28.420843854913457	21.87738851962577	25.787304443734943	23.91446318172583
14	22.38169873934539	20.65809460230846	27.42068905465417	29.539517603691984
15	24.076519703170504	28.214282258922786	24.121750403931927	23.587447633974786
16	23.31920780967308	27.954508073801023	25.052970713725948	23.673313402799952
17	22.93728654495496	27.261776913476332	26.065944910457723	23.734991631110983
18	23.64791648526011	26.823256803955147	27.054489691270234	22.474337019514508
19	24.984278098665815	26.04877175669269	26.80172989289757	22.16522025174392
20	24.616627482851033	26.089648700161572	26.794473630743333	22.499250186244062
21	23.519964395940363	26.779235480219434	26.715622248667266	22.98517787517294
22	23.233100165442778	26.49164562350642	26.798101761820455	23.477152449230353
23	23.287038380789287	26.32475159395892	27.187763039503093	23.2004469857487
24	23.34702348126433	26.678131560870366	26.462378699484322	23.512466258380982
25	23.65130274093209	26.19341324896719	27.291769463713848	22.863514546386867
26	23.53931442835167	26.45657368976093	26.901140684410645	23.10297119747676
27	23.46796118383498	26.5922657920452	26.53881132750897	23.400961696610842
28	23.260432086223744	26.376512930659157	27.13721107982856	23.22584390328854
29	23.823518029392698	26.400700471173288	26.63991524685804	23.135866252575973
30	23.722172234638492	26.390057953347075	26.66555403980302	23.22221577221142
31	23.66750839307656	26.1834963573564	26.877436894706797	23.271558354860243
32	22.990257258680906	26.30346655830649	26.935003241130428	23.771272941882177
33	22.924950899292757	26.56952950396192	27.328776400700473	23.176743196044853
34	23.598815778016426	25.821167000454725	27.024980891842993	23.55503632968585
35	23.35984287773682	26.516800665641117	26.826159308816845	23.297197147805225
36	23.780464207277547	26.453913060304373	26.482454358111053	23.283168374307028
37	23.59567139774959	25.88889211389429	26.854216855813235	23.661219632542885
38	23.49335810137482	26.401909848198997	26.585251405296106	23.51948064513008
39	23.110711210441277	26.298870925608803	27.16817113168665	23.42224673226328
40	23.194400100620168	26.286051529136312	27.286690080205883	23.232858290037637
41	23.46167242330131	25.85551330798479	27.150514227111334	23.532300041602568
42	23.224392650857688	26.446173047339855	27.068034713958145	23.26139958784431
43	23.64525585580356	25.652337967666096	27.20808057353496	23.494325602995385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	74.0
4	132.0
5	132.0
6	150.5
7	169.0
8	161.5
9	154.0
10	221.0
11	288.0
12	288.0
13	431.0
14	574.0
15	1085.5
16	1597.0
17	1814.5
18	2032.0
19	2032.0
20	1966.0
21	1900.0
22	2196.5
23	2493.0
24	3338.5
25	4184.0
26	4184.0
27	5536.5
28	6889.0
29	8907.5
30	10926.0
31	12938.0
32	14950.0
33	14950.0
34	17580.5
35	20211.0
36	22399.5
37	24588.0
38	26889.0
39	29190.0
40	29190.0
41	30650.5
42	32111.0
43	33153.5
44	34196.0
45	33754.0
46	33312.0
47	33312.0
48	33023.5
49	32735.0
50	32117.5
51	31500.0
52	30295.0
53	29090.0
54	29090.0
55	27284.5
56	25479.0
57	22943.0
58	20407.0
59	18623.5
60	16840.0
61	16840.0
62	14855.5
63	12871.0
64	10647.5
65	8424.0
66	7182.0
67	5940.0
68	5940.0
69	4942.0
70	3944.0
71	3268.5
72	2593.0
73	2086.5
74	1580.0
75	1580.0
76	1239.0
77	898.0
78	702.5
79	507.0
80	402.0
81	297.0
82	297.0
83	233.5
84	170.0
85	154.0
86	138.0
87	96.0
88	54.0
89	54.0
90	49.0
91	44.0
92	26.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.83400204185618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02774897630863	60.59302252775232
2	6.593239220615913	9.076780839486439
3	1.9523363197463088	4.031613666594222
4	0.9567124936288487	2.6341739895966994
5	0.5206006720908126	1.7917513862845347
6	0.36061261462135197	1.4893445670699141
7	0.2646564586028342	1.2752154248300542
8	0.18128634669010182	0.9982931806581693
9	0.14979420008294303	0.9279840846930761
>10	0.9155531331793858	11.716307027056747
>50	0.054115586120342396	2.4794328265199677
>100	0.02263658503032653	2.499396836699091
>500	3.5369664109885203E-4	0.20621362952309855
>1k	3.5369664109885203E-4	0.28047001323566656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.27864046672278175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	847	0.20486846815468418	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.1146489420369779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.256262154239109E-4	0.0	0.0
2	0.0	0.0	9.675016205652144E-4	0.0	0.0
3	0.0	0.0	9.675016205652144E-4	0.0	0.0
4	0.0	0.0	9.675016205652144E-4	0.0	0.0
5	0.0	2.418754051413036E-4	9.675016205652144E-4	0.0	0.0
6	0.0	2.418754051413036E-4	0.0016931278359891254	0.0	0.0
7	0.0	2.418754051413036E-4	0.004353757292543465	0.0	0.0
8	0.0	2.418754051413036E-4	0.0050793835079673754	0.0	0.0
9	0.0	2.418754051413036E-4	0.006530635938815197	7.256262154239109E-4	0.0
10	2.418754051413036E-4	2.418754051413036E-4	0.007256262154239109	7.256262154239109E-4	0.0
11	4.837508102826072E-4	2.418754051413036E-4	0.011126268636499966	7.256262154239109E-4	0.0
12	4.837508102826072E-4	2.418754051413036E-4	0.012577521067347788	9.675016205652144E-4	0.0
13	4.837508102826072E-4	2.418754051413036E-4	0.013303147282771698	9.675016205652144E-4	0.0
14	4.837508102826072E-4	2.418754051413036E-4	0.013545022687913003	9.675016205652144E-4	0.0
15	4.837508102826072E-4	2.418754051413036E-4	0.014512524308478218	9.675016205652144E-4	0.0
16	4.837508102826072E-4	4.837508102826072E-4	0.01548002592904343	0.001209377025706518	0.0
17	4.837508102826072E-4	4.837508102826072E-4	0.015963776739326037	0.0016931278359891254	0.0
18	4.837508102826072E-4	4.837508102826072E-4	0.016689402954749948	0.0016931278359891254	0.0
19	4.837508102826072E-4	4.837508102826072E-4	0.017173153765032555	0.0016931278359891254	0.0
20	4.837508102826072E-4	4.837508102826072E-4	0.018624406195880377	0.002418754051413036	0.0
21	4.837508102826072E-4	4.837508102826072E-4	0.018866281601021682	0.002418754051413036	0.0
22	4.837508102826072E-4	4.837508102826072E-4	0.019833783221586895	0.002418754051413036	0.0
23	4.837508102826072E-4	4.837508102826072E-4	0.0200756586267282	0.0026606294565543398	0.0
24	4.837508102826072E-4	4.837508102826072E-4	0.020317534031869502	0.0029025048616956434	0.0
25	4.837508102826072E-4	4.837508102826072E-4	0.020317534031869502	0.0029025048616956434	0.0
26	4.837508102826072E-4	4.837508102826072E-4	0.020317534031869502	0.0029025048616956434	0.0
27	4.837508102826072E-4	4.837508102826072E-4	0.020559409437010807	0.0033862556719782507	0.0
28	4.837508102826072E-4	4.837508102826072E-4	0.020559409437010807	0.008223763774804323	0.0
29	4.837508102826072E-4	4.837508102826072E-4	0.020559409437010807	0.021526911057576023	0.0
30	4.837508102826072E-4	4.837508102826072E-4	0.020559409437010807	0.043295697520293344	0.0
31	4.837508102826072E-4	4.837508102826072E-4	0.020559409437010807	0.10545767664160838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACCC	25	0.0054923245	29.599998	24
CGCTTTA	25	0.0054923245	29.599998	23
GTATCAA	780	0.0	24.666664	1
GGTATCA	280	0.0	24.446428	1
CAATTAC	40	0.0019291076	23.125	1
AATACGC	40	0.0019291076	23.125	5
CGCGCGA	50	2.698049E-4	22.199999	32
CGCGATA	50	2.698049E-4	22.199999	34
CTTAGGC	50	2.698049E-4	22.199999	3
ATACGCC	45	0.0038217264	20.555557	6
GTATAGA	55	5.13544E-4	20.181818	1
AATATAG	65	6.888474E-5	19.923077	5
GCGATAC	75	9.242256E-6	19.733334	35
GGACTAG	70	1.2168026E-4	18.5	19
TAGCACC	60	9.2231855E-4	18.5	4
ACGGGAC	115	6.375558E-8	17.695652	16
TGTGCAA	105	4.778922E-7	17.619047	10
CGATACA	85	2.7162918E-5	17.411764	36
GGACTGA	75	2.0632346E-4	17.266666	6
TTAACGG	75	2.0632346E-4	17.266666	35
>>END_MODULE