FastQCFastQC Report
Fri 10 Feb 2017
ERR1633082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289457
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64382.224164556393523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57221.9768048449337896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26810.9262170201446157No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG15350.530303292026104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.5033562843531163No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11680.4035141661801234No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11480.3966046770332035No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10200.35238394649291604No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9650.3333828513388863No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9530.3292371578507343No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9120.31507270509954843No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8030.27741598924883487No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7950.27465219359006693No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.26566985769907103No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6920.23906832448342932No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6910.23872285002608332No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6630.22904956522039546No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6240.21557606138390156No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5650.1951930684004878No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5490.18966547708295187No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5360.18517430913745392No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5210.17999219227726398No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5140.177573871075842No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5050.17446460095972804No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.17239175421565206No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4960.1713553308436141No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4900.1692824840995381No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4740.16375489278200217No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4720.16306394386731016No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4660.1609910971232342No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4620.1596091992938502No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4600.15891825037915822No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4560.15753635254977422No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4550.15719087809242824No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4470.15442708243366027No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4400.1520087612322383No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4310.14889949111612433No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4260.14717211882939435No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4220.14579022100001038No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4180.14440832317062638No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4160.1437173742559344No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4110.1419900019692044No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4090.1412990530545124No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4020.13888073185309044No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3950.13646241065166848No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3920.13542598727963048No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.13473503836493847No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3900.13473503836493847No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3880.13404408945024648No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3840.1326621916208625No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3830.1323167171635165No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3740.12920744704740256No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3740.12920744704740256No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG3690.12748007476067255No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA3670.12678912584598057No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.1264436513886346No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3660.1264436513886346No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG3600.12437080464455862No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3520.12160700898579063No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3510.12126153452844464No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3470.11987963669906065No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3400.11746131549763869No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3400.11746131549763869No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3380.11677036658294668No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3380.11677036658294668No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3350.11573394321090871No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3340.11538846875356272No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3290.11366109646683273No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3190.11020635189337277No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3190.11020635189337277No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3110.10744255623460479No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC3090.1067516073199128No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3030.10467876057583683No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2980.10295138828910685No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2900.10018759263033887No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2900.10018759263033887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC200.001839662437.02
CAGTTGC200.001839662437.020
GTGTGGA200.001839662437.027
CTTTGAA200.001839662437.020
GGTGCAT251.2313448E-437.07
GACGCCA251.2313448E-437.032
AAGTGTG200.001839662437.025
AGGTGCA200.001839662437.06
GCTCAAG200.001839662437.01
CTCAAGG451.0752228E-732.888892
GGTATCA8800.030.2727281
AGCCAGC250.005489635729.627
AAGGTGC250.005489635729.65
GGTCAAC250.005489635729.618
CCTCGTA250.005489635729.630
GGCTTTG250.005489635729.618
GAAGTGT250.005489635729.624
GGACGCC250.005489635729.631
GGTTGGC250.005489635729.624
CAGTCTG250.005489635729.637
Produced by FastQC (version 0.11.5)