FastQCFastQC Report
Fri 10 Feb 2017
ERR1633081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501543
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47880.9546539379474941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35080.6994415234586067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18100.3608863048631922No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9060.18064253713041553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.16927760929770727No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7740.15432375688624902No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7730.15412437218742958No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7080.14116436676416577No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6990.1393699044747908No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6840.13637913399249915No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6790.13538221049840193No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6770.13498344110076305No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6640.13239144001611028No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6580.13119513182319362No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6420.12800497664208255No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6400.12760620724444363No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6400.12760620724444363No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6060.12082712748458257No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6010.11983020399048536No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5940.11843451109874926No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5830.1162412794117354No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5470.10906343025423543No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.10627204447076323No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5320.10607265977194379No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5280.105275120976666No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5260.10487635157902712No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5200.10368004338611046No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5040.10048988820499938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTCG200.00184094737.024
GGTATCA8300.027.4156631
AACCACG358.862398E-426.4285735
GCCGGCA1750.025.37142815
GTATCAA26100.024.7375471
CCGGCAG1800.024.66666816
GCCGTCC1650.024.66666627
GGCAGCT1850.024.018
TAACCAC502.6991605E-422.19999934
CGCCGTC1850.022.026
CCGCTCT2200.021.86363628
TCCGCCG1700.021.76470631
GTACTGG1700.021.7647061
CGGCAGC2050.021.65853717
GTCCGGC2050.021.65853715
AGCTTCG2150.021.51162721
GCCGCTC2150.021.51162727
CGCTCTC2150.021.51162729
TCGCCGT1900.021.42105325
GAGTCGC1900.021.42105322
Produced by FastQC (version 0.11.5)