##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501543
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76252484831809	33.0	33.0	33.0	27.0	33.0
2	31.27550578913473	33.0	33.0	33.0	27.0	33.0
3	31.708816591997095	33.0	33.0	33.0	27.0	33.0
4	31.829384519373214	33.0	33.0	33.0	27.0	33.0
5	31.955411599803007	33.0	33.0	33.0	33.0	33.0
6	34.80102204596615	37.0	37.0	37.0	27.0	37.0
7	34.93912386375645	37.0	37.0	37.0	27.0	37.0
8	35.15639536390698	37.0	37.0	37.0	33.0	37.0
9	35.196469694522705	37.0	37.0	37.0	33.0	37.0
10	35.25100739119078	37.0	37.0	37.0	33.0	37.0
11	35.31472077169854	37.0	37.0	37.0	33.0	37.0
12	35.181902648426956	37.0	37.0	37.0	33.0	37.0
13	35.262615568356054	37.0	37.0	37.0	33.0	37.0
14	35.211281983798	37.0	37.0	37.0	33.0	37.0
15	35.25512867291538	37.0	37.0	37.0	33.0	37.0
16	35.26864296780136	37.0	37.0	37.0	33.0	37.0
17	35.248469223974816	37.0	37.0	37.0	33.0	37.0
18	35.201623788987185	37.0	37.0	37.0	33.0	37.0
19	35.24761386361688	37.0	37.0	37.0	33.0	37.0
20	35.17718919414686	37.0	37.0	37.0	33.0	37.0
21	35.233094669848846	37.0	37.0	37.0	33.0	37.0
22	35.23283546974038	37.0	37.0	37.0	33.0	37.0
23	35.21772609726384	37.0	37.0	37.0	33.0	37.0
24	35.22017653521233	37.0	37.0	37.0	33.0	37.0
25	35.231473672247446	37.0	37.0	37.0	33.0	37.0
26	35.18908448527843	37.0	37.0	37.0	33.0	37.0
27	35.123498882448764	37.0	37.0	37.0	33.0	37.0
28	35.10983305519168	37.0	37.0	37.0	33.0	37.0
29	35.14001790474595	37.0	37.0	37.0	33.0	37.0
30	35.09752304388657	37.0	37.0	37.0	33.0	37.0
31	35.07969406411813	37.0	37.0	37.0	27.0	37.0
32	35.10186963032083	37.0	37.0	37.0	33.0	37.0
33	35.08200293893046	37.0	37.0	37.0	33.0	37.0
34	35.00566451929346	37.0	37.0	37.0	27.0	37.0
35	34.988405779763646	37.0	37.0	37.0	27.0	37.0
36	34.96185770711584	37.0	37.0	37.0	27.0	37.0
37	34.96527516085361	37.0	37.0	37.0	27.0	37.0
38	34.81300905405917	37.0	37.0	37.0	27.0	37.0
39	34.86482514958837	37.0	37.0	37.0	27.0	37.0
40	34.86790763703212	37.0	37.0	37.0	27.0	37.0
41	34.880305377604714	37.0	37.0	37.0	27.0	37.0
42	34.713270846168726	37.0	37.0	37.0	27.0	37.0
43	34.52664876192071	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	27.0
17	37.0
18	22.0
19	25.0
20	47.0
21	115.0
22	278.0
23	658.0
24	1405.0
25	2407.0
26	3971.0
27	6224.0
28	8811.0
29	12098.0
30	16041.0
31	20435.0
32	25716.0
33	33406.0
34	47469.0
35	85969.0
36	236372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33375802274182	20.36495375271911	14.103676055692135	23.197612168846938
2	16.138197522445733	23.795965650004085	38.7095423523008	21.356294475249378
3	17.351852184159682	28.61130551119246	30.947894796657515	23.088947507990344
4	11.738574758295899	18.349174447654537	41.18988800561467	28.72236278843489
5	13.401443146450054	37.12184199560157	35.05661528522978	14.42009957271859
6	26.987516524006917	42.49366455119501	16.662579280340868	13.856239644457204
7	25.508480828164288	33.52414449010354	23.172689081494507	17.794685600237667
8	24.227832907647002	34.71267667976624	20.445903940439802	20.613586472146956
9	27.062285786064205	14.43086634645484	20.28161094861258	38.22523691886837
10	15.721882271310736	27.487772733344894	33.38497397032757	23.405371025016798
11	33.51975802672951	24.620620764321306	23.481535979965827	18.378085228983355
12	23.72618100541728	27.22498370030087	29.035994919677876	20.01284037460397
13	28.66035414710204	23.160327230167702	25.714046452647132	22.46527217008312
14	22.660270405528536	21.655570908177367	28.832024372785586	26.852134313508515
15	24.57795243877394	28.217121961626425	26.32137224525116	20.88355335434848
16	21.63962013227181	29.013065679313637	25.81812526543088	23.529188922983675
17	21.996518743158614	26.578179737330597	26.306817162237333	25.118484357273452
18	23.64961728107062	25.80117756603123	28.619280899145238	21.92992425375292
19	25.956099477013932	24.972734142436444	28.707209551324613	20.36395682922501
20	25.230737942708803	25.16773237788186	28.717976325060864	20.88355335434848
21	22.830345553621523	27.200259997647258	28.56185810588524	21.40753634284598
22	22.40844753091958	26.90377495050275	28.774202810127946	21.913574708449723
23	22.22940007137972	26.59991266950192	29.769929996032246	21.400757263086117
24	22.64591470721354	27.1514107464365	27.782662702898854	22.42001184345111
25	22.617402695282358	26.833591536518302	29.446727399245926	21.10227836895341
26	22.38053367308486	27.11791411703483	28.89044408953968	21.611108120340628
27	22.450916471768124	26.832195843626565	28.50084638804649	22.21604129655882
28	21.640018901669446	26.69900686481518	29.536649898413497	22.124324335101875
29	23.538560003828184	26.603700978779486	28.083534213417394	21.774204803974932
30	23.153946919805477	26.28209345958372	29.056132774258636	21.507826846352156
31	22.53844635454986	26.571600042269555	28.896226245805444	21.99372735737514
32	21.057416811719033	26.295252849705808	29.368767982007526	23.278562356567633
33	21.07296881822695	26.394945199115526	30.164911084393566	22.367174898263958
34	22.218035143547013	25.986605335933312	29.648704099150024	22.146655421369655
35	22.048757534249305	27.159784903786914	28.722163403736072	22.06929415822771
36	22.886970808086247	27.015829151239274	28.265971212837183	21.831228827837293
37	22.32390841862014	26.431831368397123	28.602731969143225	22.641528243839513
38	22.35082535296076	26.730310262529834	28.350310940437808	22.568553444071597
39	21.652380752996255	26.65693669336428	28.946670574606763	22.744011979032706
40	21.697641079628266	27.441914252616428	29.548612980342664	21.311831687412646
41	22.349629044767845	25.955900092315115	28.82624221651982	22.868228646397217
42	21.207154720532436	27.546391834797813	28.88007608520107	22.366377359468682
43	22.43456692646493	25.96447363436435	28.690461236623776	22.91049820254694
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	50.0
2	64.0
3	276.5
4	489.0
5	489.0
6	618.0
7	747.0
8	721.0
9	695.0
10	954.0
11	1213.0
12	1213.0
13	1791.0
14	2369.0
15	4238.5
16	6108.0
17	6595.0
18	7082.0
19	7082.0
20	5755.5
21	4429.0
22	3948.5
23	3468.0
24	4543.0
25	5618.0
26	5618.0
27	6918.0
28	8218.0
29	10440.0
30	12662.0
31	15492.0
32	18322.0
33	18322.0
34	21728.5
35	25135.0
36	28034.5
37	30934.0
38	33370.5
39	35807.0
40	35807.0
41	38124.5
42	40442.0
43	41724.0
44	43006.0
45	41896.0
46	40786.0
47	40786.0
48	39912.0
49	39038.0
50	39433.5
51	39829.0
52	37312.0
53	34795.0
54	34795.0
55	32989.0
56	31183.0
57	26416.5
58	21650.0
59	19822.0
60	17994.0
61	17994.0
62	14884.5
63	11775.0
64	9029.5
65	6284.0
66	5402.0
67	4520.0
68	4520.0
69	3628.0
70	2736.0
71	2195.0
72	1654.0
73	1444.5
74	1235.0
75	1235.0
76	819.0
77	403.0
78	338.0
79	273.0
80	217.5
81	162.0
82	162.0
83	155.5
84	149.0
85	126.5
86	104.0
87	89.5
88	75.0
89	75.0
90	57.5
91	40.0
92	25.5
93	11.0
94	7.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	501543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.22730778940922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.69267454389164	40.5379625701697
2	5.51465124251997	5.098549606784373
3	1.6682829633431067	2.313606900788685
4	0.9484019511708406	1.753682756194028
5	0.6455926883319237	1.4922005955055988
6	0.45710911494213524	1.2678554249864713
7	0.33028985940089506	1.068788769317211
8	0.273132045731028	1.010092731612738
9	0.2286374328589088	0.9512363682854247
>10	1.8269977718279646	16.882835785497875
>50	0.2276086962973913	7.358765974365446
>100	0.17441279409739038	14.988839579961319
>500	0.010900799631086899	3.238558477868246
>1k	0.001308095955730428	2.03702445866288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4788	0.9546539379474941	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3508	0.6994415234586067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1810	0.3608863048631922	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	906	0.18064253713041553	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	849	0.16927760929770727	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	774	0.15432375688624902	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	773	0.15412437218742958	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	708	0.14116436676416577	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	699	0.1393699044747908	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	684	0.13637913399249915	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	679	0.13538221049840193	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	677	0.13498344110076305	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	664	0.13239144001611028	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	658	0.13119513182319362	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	642	0.12800497664208255	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	640	0.12760620724444363	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	640	0.12760620724444363	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	606	0.12082712748458257	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	601	0.11983020399048536	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	594	0.11843451109874926	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	583	0.1162412794117354	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	547	0.10906343025423543	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.10627204447076323	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	532	0.10607265977194379	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	528	0.105275120976666	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	526	0.10487635157902712	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	520	0.10368004338611046	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	504	0.10048988820499938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.993846988194432E-4	0.0	0.0
2	1.993846988194432E-4	0.0	1.993846988194432E-4	0.0	0.0
3	1.993846988194432E-4	0.0	7.975387952777728E-4	0.0	0.0
4	1.993846988194432E-4	0.0	0.001196308192916659	0.0	0.0
5	3.987693976388864E-4	0.0	0.0027913857834722046	0.0	0.0
6	3.987693976388864E-4	0.0	0.005782156265763853	0.0	0.0
7	3.987693976388864E-4	0.0	0.015352621809097126	0.0	0.0
8	3.987693976388864E-4	0.0	0.018542776990208218	0.0	0.0
9	3.987693976388864E-4	0.0	0.021932316870138753	1.993846988194432E-4	0.0
10	9.96923494097216E-4	0.0	0.024723702653610957	1.993846988194432E-4	0.0
11	9.96923494097216E-4	0.0	0.03927878566743031	3.987693976388864E-4	0.0
12	9.96923494097216E-4	0.0	0.043266479643819175	5.981540964583295E-4	0.0
13	9.96923494097216E-4	0.0	0.04545971133083305	5.981540964583295E-4	0.0
14	9.96923494097216E-4	0.0	0.04805171241548581	5.981540964583295E-4	0.0
15	9.96923494097216E-4	0.0	0.05343509928361078	5.981540964583295E-4	0.0
16	0.001196308192916659	0.0	0.05762217795881908	7.975387952777728E-4	0.0
17	0.001196308192916659	0.0	0.05921725554937463	7.975387952777728E-4	0.0
18	0.0013956928917361023	0.0	0.0600147943446524	7.975387952777728E-4	0.0
19	0.0013956928917361023	0.0	0.06180925663402739	9.96923494097216E-4	0.0
20	0.0013956928917361023	0.0	0.062407410730485724	0.001196308192916659	0.0
21	0.0013956928917361023	0.0	0.0642018730198607	0.0015950775905555455	0.0
22	0.0013956928917361023	0.0	0.06559756591159681	0.0021932316870138754	0.0
23	0.0013956928917361023	0.0	0.06579695061041625	0.0035889245787499775	0.0
24	0.0013956928917361023	0.0	0.06679387410451347	0.00498461747048608	0.0
25	0.0013956928917361023	0.0	0.06699325880333291	0.005582771566944409	0.0
26	0.0013956928917361023	0.0	0.06719264350215236	0.005981540964583296	0.0
27	0.0013956928917361023	0.0	0.06719264350215236	0.008573542049236058	0.0
28	0.0013956928917361023	0.0	0.06719264350215236	0.019140931086666545	0.0
29	0.0013956928917361023	0.0	0.06719264350215236	0.05303632988597189	0.0
30	0.0013956928917361023	0.0	0.06719264350215236	0.10647142916958267	0.0
31	0.0013956928917361023	0.0	0.0673920282009718	0.21812686050847085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCTCG	20	0.001840947	37.0	24
GGTATCA	830	0.0	27.415663	1
AACCACG	35	8.862398E-4	26.42857	35
GCCGGCA	175	0.0	25.371428	15
GTATCAA	2610	0.0	24.737547	1
CCGGCAG	180	0.0	24.666668	16
GCCGTCC	165	0.0	24.666666	27
GGCAGCT	185	0.0	24.0	18
TAACCAC	50	2.6991605E-4	22.199999	34
CGCCGTC	185	0.0	22.0	26
CCGCTCT	220	0.0	21.863636	28
TCCGCCG	170	0.0	21.764706	31
GTACTGG	170	0.0	21.764706	1
CGGCAGC	205	0.0	21.658537	17
GTCCGGC	205	0.0	21.658537	15
AGCTTCG	215	0.0	21.511627	21
GCCGCTC	215	0.0	21.511627	27
CGCTCTC	215	0.0	21.511627	29
TCGCCGT	190	0.0	21.421053	25
GAGTCGC	190	0.0	21.421053	22
>>END_MODULE