FastQCFastQC Report
Fri 10 Feb 2017
ERR1633080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318895
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8420.26403675190893555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.17842863638501702No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4270.13389987299894948No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3850.12072939368757742No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3730.11696639959861398No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3670.11508490255413223No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3620.11351698835039747No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3480.10912682857994012No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3430.10755891437620534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3320.10410950312798883No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3310.1037959202872419No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3260.10222800608350711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATG250.005490461429.62
GGACCGT250.005490461429.66
GGTATCA2350.029.1276571
CCGCAAC358.855287E-426.42857217
CGAGTTC400.001928144423.12500214
GCGGTGC400.001928144423.12500217
CCGAGTT400.001928144423.12500213
TTGCCGA400.001928144423.12500210
TGCCGAG400.001928144423.12500211
GACCGCA502.696172E-422.215
TATTAGA1201.0913936E-1121.5833322
GTATATA705.0828676E-621.1428591
GTATCAA6050.021.0991731
AGACCGC450.003819838420.55555514
TCCGTAG450.003819838420.55555534
TATACTG450.003819838420.5555555
CGCAACC450.003819838420.55555518
GTGTTAT555.1318994E-420.1818181
TCTGACG555.1318994E-420.18181836
ACTGTGT555.1318994E-420.1818188
Produced by FastQC (version 0.11.5)