FastQCFastQC Report
Fri 10 Feb 2017
ERR1633079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406011
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60631.4933092945757627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47721.1753376140055318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22020.542349837812276No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11850.2918640135365791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.2748694000901453No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10810.26624894399412824No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10750.2647711515205253No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10170.25048582427569693No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8240.20295016637480265No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7390.18201477299876112No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7330.18053698052515818No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6870.16920723822753572No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6700.1650201595523274No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6670.16428126331552592No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6530.1608330808771191No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.16009418464031763No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6300.15516820972830786No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6240.15369041725470492No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6000.1477792473602932No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5940.14630145488669025No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5930.14605515614108977No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5910.1455625586498888No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5860.14433106492188635No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5810.14309957119388392No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5760.14186807746588145No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5490.13521801133466826No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5460.1344791150978668No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5270.1297994389314575No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5270.1297994389314575No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5240.12906054269465606No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5170.12733645147545264No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4990.12290307405464385No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4950.12191787907224187No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4930.1214252815810409No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4820.11871599537943553No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4770.11748450165143308No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4710.11600670917783017No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4690.11551411168662917No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4620.11379002046742577No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4540.11181963050262186No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4530.11157333175702136No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4530.11157333175702136No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4450.10960294179221747No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4440.10935664304661696No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4380.10787885057301402No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4370.10763255182741353No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4330.10664735684501159No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4300.10590846060821013No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4300.10590846060821013No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4290.10566216186260963No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4280.10541586311700914No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4200.10344547315220523No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4170.10270657691540377No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4170.10270657691540377No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4150.10221397942420278No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4140.10196768067860229No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4140.10196768067860229No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4090.10073618695059987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCTA303.5954153E-430.83333226
CTCTGTG303.5954153E-430.83333223
AACTCTC303.5954153E-430.83333219
CTCTCTG303.5954153E-430.83333221
AGTAACC303.5954153E-430.83333232
GCTATAC250.005492208529.63
CCCTTAA250.005492208529.618
TAGTGCA502.7134593E-729.610
CTAGTGC453.9998667E-628.7777779
TAACCAC358.8594755E-426.42857234
GTGCTAG358.8594755E-426.42857227
CTTTCAT358.8594755E-426.42857220
CCACGTT358.8594755E-426.42857237
GTAACCA358.8594755E-426.42857233
CTGTGCT358.8594755E-426.42857225
CATTACG509.07219E-625.9000022
ACTAGTG951.0913936E-1125.315798
GCCGGCA3000.025.28333315
GTATCAA24250.025.022681
GCCGCTC2850.024.66666627
Produced by FastQC (version 0.11.5)