FastQCFastQC Report
Fri 10 Feb 2017
ERR1633078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397796
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8200.2061358083037537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.13172580920874014No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4630.1163913161519975No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4270.10734145139719856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT358.8591577E-426.42857223
GACGTAG358.8591577E-426.42857225
AGACGTA358.8591577E-426.42857224
ACGTAGA358.8591577E-426.42857226
GTATCAA5300.021.9905661
CGTGCAA705.0880917E-621.14285911
TATACAG705.0880917E-621.1428595
GGTATCA2550.021.0392151
CTAGATT450.003821477320.55555532
TTATGCG450.003821477320.5555554
ACGAGCA450.003821477320.55555536
CTCGCTT555.1349704E-420.18181824
TAACCGT759.240812E-619.7333347
ATTAACC701.2166551E-418.53
TATGCGG500.007026992318.55
AGCTAGT500.007026992318.521
CTCTAAT902.145025E-618.51
GTTAGGT1054.777794E-717.6190471
CTAACCG852.7158738E-517.4117646
ACCGTGC852.7158738E-517.4117649
Produced by FastQC (version 0.11.5)