FastQCFastQC Report
Fri 10 Feb 2017
ERR1633077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438633
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35400.8070528209231863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.6064295208066881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14810.3376398948551523No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8030.1830687613563047No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7370.16802201384756732No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7210.1643743174818128No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7090.16163854520749693No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7060.16095460213891796No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6980.1591307539560407No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6950.15844681088746174No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6620.15092343713309303No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6460.14727574076733851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.14294410133300503No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6150.14020832905868916No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6060.13815649985295222No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6050.13792851883009258No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6040.13770053780723293No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5940.13542072757863635No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5570.12698542973282903No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5470.12470561950423247No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5300.1208299421156183No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5120.11672628370414447No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5040.1149024355212672No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4970.11330656836124962No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4910.11193868222409166No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4700.10715108074403888No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4640.10578319460688092No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4630.10555521358402127No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4580.10441530846972298No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4450.10145155517254745No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4400.10031165005824916No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4390.10008366903538948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.028.842521
GTATCAA18700.023.8422471
GCCGTCC1400.022.46428527
CGCTCTC1600.020.812529
GGGGTCG450.00382209320.55555528
GGACCGT450.00382209320.5555556
CCGCTCT1650.020.18181828
GCCGCTC1650.020.18181827
CGGGAAT555.136128E-420.18181817
AGTCGCC1750.020.08571423
GGCAGCT1850.020.018
CCGGCAG1950.019.92307716
GCCGGCA1950.019.92307715
TCGGCGT656.889697E-519.92307711
CGCCGGC1950.019.92307714
CGCCGCC1500.019.73333433
TATACAG759.244362E-619.7333345
GTATAGA951.6702143E-719.4736841
CGGCAGC2000.019.42500117
ACTGCTC3850.019.220788
Produced by FastQC (version 0.11.5)