##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438633
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.442424532581907	33.0	33.0	33.0	27.0	33.0
2	31.039142061814776	33.0	33.0	33.0	27.0	33.0
3	31.456869410190297	33.0	33.0	33.0	27.0	33.0
4	31.60689916171378	33.0	33.0	33.0	27.0	33.0
5	31.729372847004214	33.0	33.0	33.0	27.0	33.0
6	34.5244110680227	37.0	33.0	37.0	27.0	37.0
7	34.735480458606624	37.0	37.0	37.0	27.0	37.0
8	34.910923254748276	37.0	37.0	37.0	27.0	37.0
9	35.00496998629834	37.0	37.0	37.0	27.0	37.0
10	35.01814044998894	37.0	37.0	37.0	27.0	37.0
11	35.03752795617293	37.0	37.0	37.0	27.0	37.0
12	34.98515159598115	37.0	37.0	37.0	27.0	37.0
13	35.02967856955587	37.0	37.0	37.0	27.0	37.0
14	34.99419104353753	37.0	37.0	37.0	27.0	37.0
15	35.01021127001388	37.0	37.0	37.0	27.0	37.0
16	35.00807508782969	37.0	37.0	37.0	27.0	37.0
17	34.98994375708166	37.0	37.0	37.0	27.0	37.0
18	34.97541680630504	37.0	37.0	37.0	27.0	37.0
19	34.975152348318524	37.0	37.0	37.0	27.0	37.0
20	34.97498364236161	37.0	37.0	37.0	27.0	37.0
21	34.97267647441027	37.0	37.0	37.0	27.0	37.0
22	34.9651371419843	37.0	37.0	37.0	27.0	37.0
23	34.95565085162311	37.0	37.0	37.0	27.0	37.0
24	34.93959186837288	37.0	37.0	37.0	27.0	37.0
25	34.93861838940526	37.0	37.0	37.0	27.0	37.0
26	34.91030542617632	37.0	37.0	37.0	27.0	37.0
27	34.840322091589094	37.0	37.0	37.0	27.0	37.0
28	34.81447816283772	37.0	37.0	37.0	27.0	37.0
29	34.84880754525993	37.0	37.0	37.0	27.0	37.0
30	34.799668515592764	37.0	37.0	37.0	27.0	37.0
31	34.76063588466896	37.0	37.0	37.0	27.0	37.0
32	34.78684914267736	37.0	37.0	37.0	27.0	37.0
33	34.76195589479132	37.0	37.0	37.0	27.0	37.0
34	34.69931810876063	37.0	37.0	37.0	27.0	37.0
35	34.68337539583205	37.0	37.0	37.0	27.0	37.0
36	34.66627681911758	37.0	37.0	37.0	27.0	37.0
37	34.67029840436082	37.0	37.0	37.0	27.0	37.0
38	34.50554335857083	37.0	37.0	37.0	27.0	37.0
39	34.565376978020346	37.0	37.0	37.0	27.0	37.0
40	34.58068590370537	37.0	37.0	37.0	27.0	37.0
41	34.604778938201186	37.0	37.0	37.0	27.0	37.0
42	34.47208942327641	37.0	37.0	37.0	27.0	37.0
43	34.21343583360121	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	19.0
16	38.0
17	29.0
18	18.0
19	28.0
20	51.0
21	129.0
22	386.0
23	855.0
24	1607.0
25	2915.0
26	4751.0
27	6804.0
28	9302.0
29	12467.0
30	15855.0
31	19702.0
32	24862.0
33	31990.0
34	44150.0
35	77213.0
36	185460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84432087873006	20.663287987907886	13.917557502513493	23.57483363084857
2	16.274425316836627	23.654854969872307	38.42551745992663	21.645202253364427
3	17.161043514737834	28.494892085182826	31.00747093811911	23.336593461960227
4	11.615177152653814	18.72020573007503	41.11022198512196	28.554395132149203
5	13.105489099087391	37.26304222436524	35.72485426313114	13.906614413416227
6	27.06408318571562	42.60395364689843	16.80288532782531	13.529077839560635
7	25.49010220389255	33.465334345569076	23.459247252258724	17.585316198279656
8	23.83906363634291	35.43828211739655	20.500053575540374	20.22260067072017
9	26.414793232611316	14.556816290611971	20.53539063408362	38.492999842693095
10	15.45483353965616	27.534863997920812	33.90351387150534	23.10678859091769
11	33.45553116158611	24.406052440194877	23.809654084394015	18.328762313825
12	23.316303150925716	27.439339949342617	29.07875148472641	20.165605415005256
13	28.072443249823888	23.1412137253695	25.835949415570646	22.950393609235967
14	22.09637669760369	21.65728524757599	28.995766392405496	27.250571662414817
15	24.49998062161306	28.172754899882136	26.358253938942124	20.96901053956269
16	21.670508146901852	29.102917473149535	25.907079494702863	23.319494885245753
17	21.494050835208476	26.980642131348986	26.48569533072067	25.039611702721864
18	22.943326197527316	26.523996142561096	28.684116334156347	21.848561325755245
19	25.60090098100234	25.370868128936948	28.71329790508238	20.31493298497833
20	24.794760084170594	25.52726311061867	28.74658313441989	20.931393670790843
21	22.584940029591937	27.547402954178096	28.556902923400656	21.310754092829313
22	22.14972425695285	27.055419906846957	28.79468713024328	22.000168705956916
23	21.917411594658862	26.904268488691002	29.59056888104634	21.587751035603798
24	22.40187126823563	27.721808436665736	27.373681414759037	22.5026388803396
25	22.381124995155403	27.249659738323384	29.237426276636736	21.13178898988448
26	22.326637530691944	27.427484936153913	28.833215923106557	21.41266161004758
27	21.99674899061402	27.181493412488344	28.353999813055562	22.467757783842075
28	21.44435097222507	26.914071672673966	29.634569218458257	22.007008136642707
29	23.57278180164283	26.68084708628854	28.010660392628917	21.735710719439712
30	23.145089402758114	26.5335713455212	28.96567289738802	21.355666354332666
31	22.637831626895377	26.946672958942898	28.548695606577706	21.866799807584016
32	20.685174166102414	26.458337607977512	29.6450563455098	23.211431880410274
33	20.902668061910525	26.561385030310074	30.136355449772363	22.399591458007034
34	22.284233060440048	26.237196015803644	29.746051938636626	21.73251898511968
35	21.850613154960982	27.44367158877695	28.63327656605864	22.072438690203427
36	23.050933240317075	27.093264756641656	28.19395713500808	21.661844868033185
37	22.108459691815252	26.632743090465148	28.522021826903128	22.73677539081647
38	22.153599934341464	27.06339924264704	28.24821661844868	22.534784204562815
39	21.557429559563463	26.886941930953668	29.009901215822797	22.545727293660075
40	21.471480713945372	27.793622458866523	29.550672202045902	21.184224625142203
41	22.075630424523464	26.26706152979826	28.74863496362563	22.90867308205265
42	21.230960734828432	27.887778621307564	28.737007931459786	22.14425271240422
43	22.587447820843394	25.97364995337788	28.63373252810436	22.805169697674366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	31.5
2	36.0
3	176.5
4	317.0
5	317.0
6	420.0
7	523.0
8	497.5
9	472.0
10	645.0
11	818.0
12	818.0
13	1321.0
14	1824.0
15	3224.0
16	4624.0
17	4961.5
18	5299.0
19	5299.0
20	4400.5
21	3502.0
22	3175.5
23	2849.0
24	3675.0
25	4501.0
26	4501.0
27	5943.5
28	7386.0
29	9283.0
30	11180.0
31	14076.5
32	16973.0
33	16973.0
34	20678.0
35	24383.0
36	26715.0
37	29047.0
38	31143.0
39	33239.0
40	33239.0
41	35392.5
42	37546.0
43	38111.5
44	38677.0
45	37564.5
46	36452.0
47	36452.0
48	35248.5
49	34045.0
50	33635.0
51	33225.0
52	31332.0
53	29439.0
54	29439.0
55	28062.5
56	26686.0
57	22145.5
58	17605.0
59	16456.5
60	15308.0
61	15308.0
62	12611.0
63	9914.0
64	7395.5
65	4877.0
66	4209.0
67	3541.0
68	3541.0
69	2740.5
70	1940.0
71	1532.5
72	1125.0
73	963.0
74	801.0
75	801.0
76	518.0
77	235.0
78	159.0
79	83.0
80	68.5
81	54.0
82	54.0
83	48.5
84	43.0
85	31.5
86	20.0
87	15.0
88	10.0
89	10.0
90	7.5
91	5.0
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	438633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.97235068478747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07802694508065	42.732823469183565
2	5.093008800955072	4.886472084802514
3	1.4655342742810624	2.1091537243915988
4	0.7864376791484877	1.5090905654336648
5	0.5395899436519427	1.2942699001427853
6	0.38263094784743484	1.1013423607793864
7	0.26623304277564824	0.8940277424337996
8	0.23795671481193717	0.9132274376606568
9	0.1861616250903564	0.8037549686596056
>10	1.5417100390573244	15.107014964583731
>50	0.22265611390209672	7.449021515068102
>100	0.1889932025130084	16.503011987995652
>500	0.009617974682595848	2.9247946597303263
>1k	0.0014426962023893772	1.771994619134614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3540	0.8070528209231863	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2660	0.6064295208066881	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.3376398948551523	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	803	0.1830687613563047	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	737	0.16802201384756732	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	721	0.1643743174818128	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	709	0.16163854520749693	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	706	0.16095460213891796	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	698	0.1591307539560407	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	695	0.15844681088746174	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	662	0.15092343713309303	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	646	0.14727574076733851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.14294410133300503	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	615	0.14020832905868916	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	606	0.13815649985295222	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	605	0.13792851883009258	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	604	0.13770053780723293	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	594	0.13542072757863635	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	557	0.12698542973282903	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	547	0.12470561950423247	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	530	0.1208299421156183	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	512	0.11672628370414447	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	504	0.1149024355212672	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	497	0.11330656836124962	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	491	0.11193868222409166	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	470	0.10715108074403888	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	464	0.10578319460688092	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	463	0.10555521358402127	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	458	0.10441530846972298	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	445	0.10145155517254745	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	440	0.10031165005824916	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	439	0.10008366903538948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2798102285965716E-4	0.0
2	4.559620457193143E-4	0.0	2.2798102285965716E-4	2.2798102285965716E-4	0.0
3	4.559620457193143E-4	0.0	0.0011399051142982858	4.559620457193143E-4	0.0
4	4.559620457193143E-4	0.0	0.0018238481828772573	0.0011399051142982858	0.0
5	4.559620457193143E-4	0.0	0.0022798102285965715	0.0011399051142982858	0.0
6	4.559620457193143E-4	0.0	0.006155487617210744	0.0011399051142982858	0.0
7	4.559620457193143E-4	0.0	0.011171070120123201	0.0011399051142982858	0.0
8	6.839430685789715E-4	0.0	0.013222899325860115	0.0011399051142982858	0.0
9	9.119240914386286E-4	0.0	0.017782519783053258	0.001367886137157943	0.0
10	0.001367886137157943	0.0	0.020290311034509487	0.0015958671600176	0.0
11	0.001367886137157943	0.0	0.03465311547466789	0.0015958671600176	0.0
12	0.001367886137157943	0.0	0.03852879286328206	0.0015958671600176	0.0
13	0.0015958671600176	0.0	0.042632451274755886	0.0015958671600176	0.0
14	0.0015958671600176	0.0	0.04491226150335246	0.0015958671600176	0.0
15	0.0015958671600176	0.0	0.05197967321200183	0.0015958671600176	0.0
16	0.0015958671600176	0.0	0.05881910389779155	0.0018238481828772573	0.0
17	0.0015958671600176	0.0	0.06041497105780915	0.0018238481828772573	0.0
18	0.0015958671600176	0.0	0.061554876172107434	0.0018238481828772573	0.0
19	0.0015958671600176	0.0	0.06246680026354606	0.0018238481828772573	0.0
20	0.0015958671600176	0.0	0.06360670537784435	0.0020518292057369143	0.0
21	0.0015958671600176	0.0	0.06543055356072161	0.0025077912514562288	0.0
22	0.0015958671600176	0.0	0.06634247765216024	0.0029637532971755432	0.0
23	0.0015958671600176	0.0	0.06657045867501989	0.0031917343200352	0.0
24	0.0015958671600176	0.0	0.06748238276645852	0.004331639434333486	0.0
25	0.0015958671600176	0.0	0.06748238276645852	0.004559620457193143	0.0
26	0.0015958671600176	0.0	0.06748238276645852	0.005243563525772115	0.0
27	0.0015958671600176	0.0	0.06771036378931818	0.006155487617210744	0.0
28	0.0015958671600176	0.0	0.06793834481217784	0.02006233001164983	0.0
29	0.0015958671600176	0.0	0.06793834481217784	0.049699862983405264	0.0
30	0.0015958671600176	0.0	0.06793834481217784	0.08686076970952938	0.0
31	0.0018238481828772573	0.0	0.06793834481217784	0.18580453363062058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	635	0.0	28.84252	1
GTATCAA	1870	0.0	23.842247	1
GCCGTCC	140	0.0	22.464285	27
CGCTCTC	160	0.0	20.8125	29
GGGGTCG	45	0.003822093	20.555555	28
GGACCGT	45	0.003822093	20.555555	6
CCGCTCT	165	0.0	20.181818	28
GCCGCTC	165	0.0	20.181818	27
CGGGAAT	55	5.136128E-4	20.181818	17
AGTCGCC	175	0.0	20.085714	23
GGCAGCT	185	0.0	20.0	18
CCGGCAG	195	0.0	19.923077	16
GCCGGCA	195	0.0	19.923077	15
TCGGCGT	65	6.889697E-5	19.923077	11
CGCCGGC	195	0.0	19.923077	14
CGCCGCC	150	0.0	19.733334	33
TATACAG	75	9.244362E-6	19.733334	5
GTATAGA	95	1.6702143E-7	19.473684	1
CGGCAGC	200	0.0	19.425001	17
ACTGCTC	385	0.0	19.22078	8
>>END_MODULE