FastQCFastQC Report
Fri 10 Feb 2017
ERR1633076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449032
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11530.25677457285894995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.1828377487573269No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5200.11580466425555418No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4650.10355609399775517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4540.10110637994619537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG250.005492820429.611
GGTATCA2550.025.3921571
CGCGATA451.3214583E-424.66666634
GTATCAA7250.023.7310351
CGCGCGA400.001929364723.12532
TGCGGTA400.001929364723.12536
GCGATAC400.001929364723.12535
CTTAGCT603.7197788E-521.58333432
TAACGGC1001.2845703E-820.3536
GCGCGCG555.136389E-420.18181831
TAAGAAC555.136389E-420.1818183
CGAACCG656.8901645E-519.92307731
GCGAACC656.8901645E-519.92307730
TGCTCGC656.8901645E-519.92307710
TCTATAC759.245163E-619.7333343
GTTTCGG609.2248677E-418.521
GGTTTCG609.2248677E-418.520
AACGGCC1103.8360668E-818.537
AACAGTG902.1462783E-618.57
TCAGTAG609.2248677E-418.53
Produced by FastQC (version 0.11.5)