FastQCFastQC Report
Fri 10 Feb 2017
ERR1633075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425895
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8850.20779769661536293No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7560.1775085408375304No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7160.16811655454983035No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6910.16224656312001784No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6750.15848976860493783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.1385317977435753No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5700.1338358045997253No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4830.11340823442397774No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4750.11152983716643775No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4660.10941664025170524No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4620.10847744162293524No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4420.10378144847908521No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT4340.10190305122154521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCA250.00549250829.59999823
GGTATCA2850.027.2631571
ATCTCGT358.860192E-426.4285737
GTCGCCA809.749783E-1025.437512
TCTATAC601.3341069E-624.6666663
ATAGAAC400.001929202223.1253
CGAGACA400.001929202223.12523
GTATCAA6700.021.2611941
ACCGACC450.003821913220.5555558
CCTACGT450.003821913220.55555534
ATACTAT555.135791E-420.1818186
GCCAATA656.8890986E-519.92307731
AATAGCG759.243329E-619.7333346
CTTAAGC609.223807E-418.53
TCGCCAT1103.8346116E-818.513
TTCTATA902.145749E-618.52
GCCTTAA609.223807E-418.51
CGAACTA701.2169118E-418.529
ATAGCGG801.6127793E-518.57
TCGGGAT801.6127793E-518.522
Produced by FastQC (version 0.11.5)