FastQCFastQC Report
Fri 10 Feb 2017
ERR1633074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474849
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11870.24997420232537082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.17879367967501247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG358.861697E-426.4285729
GTATCAA7000.024.8428551
GGTATCA2400.023.1250021
ACGGGAC652.6773432E-622.7692315
TTAACGG903.814421E-922.6111135
ACGTAGT603.7203587E-521.5833341
TAACGGC957.1177055E-921.42105336
TTTAACG1001.2849341E-820.3534
GCACCGT555.1369856E-420.1818186
GCCGAGT555.1369856E-420.18181812
TTCGCCG555.1369856E-420.18181824
TACCCTC1254.110916E-1019.245
CCAATAC1002.8661088E-718.53
AACGGCC1103.837522E-818.537
ATTACCC1451.546141E-1017.8620683
GCACCGC1156.3813786E-817.6956510
ATGCCCG1054.782614E-717.61904729
CTGCTCG1502.4920155E-1017.2666669
CGAGTTC650.001578088517.07692314
CAACCCA1301.3869794E-817.07692317
Produced by FastQC (version 0.11.5)