##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633074.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474849
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73358899355374	33.0	33.0	33.0	27.0	33.0
2	31.25100821524316	33.0	33.0	33.0	27.0	33.0
3	31.70105233453161	33.0	33.0	33.0	27.0	33.0
4	31.84282161276532	33.0	33.0	33.0	27.0	33.0
5	31.931371867688465	33.0	33.0	33.0	33.0	33.0
6	34.828728711653596	37.0	37.0	37.0	27.0	37.0
7	35.003706441416114	37.0	37.0	37.0	33.0	37.0
8	35.169102177744925	37.0	37.0	37.0	33.0	37.0
9	35.26621936657759	37.0	37.0	37.0	33.0	37.0
10	35.28063236944797	37.0	37.0	37.0	33.0	37.0
11	35.31506226189799	37.0	37.0	37.0	33.0	37.0
12	35.25665211467224	37.0	37.0	37.0	33.0	37.0
13	35.29378813054255	37.0	37.0	37.0	33.0	37.0
14	35.26220335306592	37.0	37.0	37.0	33.0	37.0
15	35.296746965877574	37.0	37.0	37.0	33.0	37.0
16	35.30389871306458	37.0	37.0	37.0	33.0	37.0
17	35.263351086345345	37.0	37.0	37.0	33.0	37.0
18	35.26647418442494	37.0	37.0	37.0	33.0	37.0
19	35.27870122923287	37.0	37.0	37.0	33.0	37.0
20	35.26098401807732	37.0	37.0	37.0	33.0	37.0
21	35.277789360407205	37.0	37.0	37.0	33.0	37.0
22	35.26535593420224	37.0	37.0	37.0	33.0	37.0
23	35.24737969333409	37.0	37.0	37.0	33.0	37.0
24	35.24138831502225	37.0	37.0	37.0	33.0	37.0
25	35.25634043664407	37.0	37.0	37.0	33.0	37.0
26	35.20519575696695	37.0	37.0	37.0	33.0	37.0
27	35.16264538832345	37.0	37.0	37.0	33.0	37.0
28	35.136967751853746	37.0	37.0	37.0	33.0	37.0
29	35.16492611335393	37.0	37.0	37.0	33.0	37.0
30	35.13115537781484	37.0	37.0	37.0	33.0	37.0
31	35.133263416370255	37.0	37.0	37.0	33.0	37.0
32	35.15483659015813	37.0	37.0	37.0	33.0	37.0
33	35.12937586474858	37.0	37.0	37.0	33.0	37.0
34	35.06283049980099	37.0	37.0	37.0	33.0	37.0
35	35.06717082693656	37.0	37.0	37.0	33.0	37.0
36	35.04558291162033	37.0	37.0	37.0	27.0	37.0
37	35.084814330450314	37.0	37.0	37.0	33.0	37.0
38	34.94554479423985	37.0	37.0	37.0	27.0	37.0
39	35.01620304559976	37.0	37.0	37.0	27.0	37.0
40	35.059467325402395	37.0	37.0	37.0	27.0	37.0
41	35.08361394885532	37.0	37.0	37.0	33.0	37.0
42	35.00623777242871	37.0	37.0	37.0	27.0	37.0
43	34.79033966587273	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	23.0
17	18.0
18	12.0
19	24.0
20	30.0
21	88.0
22	237.0
23	607.0
24	1257.0
25	2263.0
26	3700.0
27	5926.0
28	8084.0
29	10965.0
30	14291.0
31	18392.0
32	23565.0
33	30727.0
34	43386.0
35	80182.0
36	231059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15687302700437	20.232115893684096	13.175556861233781	25.435454218077748
2	16.061316334245202	22.641092220895484	37.669659196923654	23.62793224793566
3	18.00509214508191	26.317629393765174	30.096514892102544	25.580763569050376
4	12.866405952207966	17.700995474350794	38.593531838542354	30.839066734898886
5	14.263902840692516	36.61437635964275	35.302801522168096	13.818919277496637
6	30.180120417227368	39.37630699443402	17.225054701599877	13.21851788673873
7	26.949409180602675	31.861286430001957	23.34973854846488	17.839565840930486
8	24.645940077793153	35.93268596964509	20.40037991024515	19.020994042316612
9	26.000054754248193	15.248426341847617	20.033526447354845	38.71799245654935
10	15.05110045509204	28.093772967827668	33.861922421654036	22.99320415542625
11	34.77021116186409	23.182106311690664	23.815992031150955	18.231690495294295
12	23.13240630179278	25.24781562138701	30.2940513721204	21.32572670469981
13	28.165374677002585	22.03795311772795	25.87959540822451	23.917076797044956
14	22.037110744678834	20.531368919382793	27.28951729918353	30.142003036754843
15	24.134830230241615	28.351328527595086	24.035430210445845	23.47841103171745
16	23.397122032477693	27.986159810803013	24.859060459219666	23.757657697499628
17	22.79440411583472	27.173269818405434	26.206436151281775	23.825889914478076
18	23.4718826405868	27.08018759647804	27.141470235801275	22.30645952713389
19	24.934452847115608	26.234866241689463	26.65078793469082	22.17989297650411
20	24.516846408016022	26.15673614138389	26.790621860844183	22.535795589755903
21	23.515686039140864	26.702804470473772	26.817577798415915	22.963931691969446
22	23.144831304267253	26.628254455626948	26.661738784329337	23.56517545577647
23	23.197058433312485	26.44819721637826	27.338374936032295	23.01636941427696
24	23.61782377134626	26.902446883114422	25.822524634146856	23.657204711392463
25	23.646464455016226	26.49410654755512	26.979945203633154	22.8794837937955
26	23.55253986003972	26.796307878925724	26.74302778356909	22.90812447746547
27	23.52937460118901	26.634151066970762	26.520851891864574	23.315622439975655
28	23.153676221282975	26.482102731605206	26.97826045753492	23.385960589576896
29	24.158206082354603	26.559811645386215	26.28856752357065	22.993414748688533
30	23.817887370511468	26.231286156230716	26.84158543031574	23.10924104294207
31	23.749655153533016	26.217808187444852	26.76661422894436	23.265922430077772
32	22.724697746020315	26.24581709132798	27.201699908813115	23.827785253838588
33	22.9906770362789	26.406920936971545	27.377545282816225	23.224856743933334
34	23.44029365124492	26.209173863691404	27.053231658906306	23.297300826157368
35	23.474830946258702	26.682166330770414	26.723442610177127	23.119560112793753
36	24.07481115049205	26.43345568801872	26.363959911466594	23.12777325002264
37	23.359847025054282	26.09440053574926	26.74387015661821	23.80188228257825
38	23.671314459965167	26.35848448664733	26.51369172094708	23.45650933244042
39	23.19642665352565	26.08366027937302	26.99131723979623	23.728595827305103
40	23.292878367649507	26.732076933930575	27.144418541473186	22.830626156946735
41	23.472935606898194	25.736181396612395	27.111565992557633	23.67931700393178
42	22.88706515123755	26.722600237128013	26.960149436978913	23.43018517465552
43	23.49883857815853	25.702065288123173	27.084188868461347	23.714907265256954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	13.5
2	22.0
3	70.5
4	119.0
5	119.0
6	147.0
7	175.0
8	172.0
9	169.0
10	225.0
11	281.0
12	281.0
13	457.0
14	633.0
15	1097.0
16	1561.0
17	1855.0
18	2149.0
19	2149.0
20	2094.0
21	2039.0
22	2455.5
23	2872.0
24	3932.0
25	4992.0
26	4992.0
27	6688.0
28	8384.0
29	10396.5
30	12409.0
31	15027.0
32	17645.0
33	17645.0
34	20600.0
35	23555.0
36	25958.5
37	28362.0
38	31198.5
39	34035.0
40	34035.0
41	35647.5
42	37260.0
43	38210.5
44	39161.0
45	38363.5
46	37566.0
47	37566.0
48	37037.5
49	36509.0
50	36176.0
51	35843.0
52	34452.5
53	33062.0
54	33062.0
55	31389.0
56	29716.0
57	26442.5
58	23169.0
59	21367.0
60	19565.0
61	19565.0
62	17137.5
63	14710.0
64	12412.0
65	10114.0
66	8559.5
67	7005.0
68	7005.0
69	5858.5
70	4712.0
71	3826.5
72	2941.0
73	2345.0
74	1749.0
75	1749.0
76	1360.0
77	971.0
78	780.5
79	590.0
80	469.0
81	348.0
82	348.0
83	262.5
84	177.0
85	163.5
86	150.0
87	113.0
88	76.0
89	76.0
90	56.0
91	36.0
92	23.5
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	474849.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.57343910405025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.54445316473546	58.28135321659895
2	7.165480785809164	9.540613974906169
3	1.9490184306422218	3.8925857941509463
4	0.9108736373998197	2.425599625236866
5	0.5640893560593169	1.8776684197428926
6	0.3479133598975949	1.3897073327176832
7	0.23332701399617906	1.0873367230323154
8	0.17355993840511472	0.9243585592252492
9	0.1408700612148212	0.8440383997681856
>10	0.853320766893128	10.853467016261583
>50	0.07381980592910539	3.4276089172292696
>100	0.04263730138548661	5.024377692843344
>500	3.1818881630960147E-4	0.17984302294464344
>1k	3.1818881630960147E-4	0.251441305341922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1187	0.24997420232537082	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	849	0.17879367967501247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.211865245583333E-4	0.0	0.0
2	0.0	0.0	4.211865245583333E-4	0.0	0.0
3	0.0	0.0	6.317797868375E-4	0.0	0.0
4	0.0	0.0	6.317797868375E-4	0.0	0.0
5	0.0	0.0	8.423730491166666E-4	0.0	0.0
6	0.0	0.0	0.0010529663113958332	0.0	0.0
7	0.0	0.0	0.002316525885070833	0.0	0.0
8	0.0	0.0	0.0027377124096291663	0.0	0.0
9	0.0	0.0	0.0031588989341874998	2.1059326227916664E-4	0.0
10	0.0	0.0	0.004211865245583333	2.1059326227916664E-4	0.0
11	0.0	0.0	0.006107204606095833	2.1059326227916664E-4	0.0
12	0.0	0.0	0.0063177978683749995	2.1059326227916664E-4	0.0
13	0.0	0.0	0.0063177978683749995	2.1059326227916664E-4	0.0
14	0.0	0.0	0.0067389843929333325	2.1059326227916664E-4	0.0
15	0.0	0.0	0.006949577655212499	2.1059326227916664E-4	0.0
16	0.0	0.0	0.0082131372288875	2.1059326227916664E-4	0.0
17	0.0	0.0	0.0082131372288875	6.317797868375E-4	0.0
18	0.0	0.0	0.0082131372288875	6.317797868375E-4	0.0
19	0.0	0.0	0.008844917015724998	6.317797868375E-4	0.0
20	0.0	0.0	0.009055510278004166	6.317797868375E-4	0.0
21	0.0	0.0	0.009687290064841666	6.317797868375E-4	0.0
22	0.0	0.0	0.009897883327120832	8.423730491166666E-4	0.0
23	0.0	0.0	0.009897883327120832	8.423730491166666E-4	0.0
24	0.0	0.0	0.0101084765894	0.001263559573675	0.0
25	0.0	0.0	0.0101084765894	0.001263559573675	0.0
26	0.0	0.0	0.0101084765894	0.001263559573675	0.0
27	0.0	0.0	0.010319069851679165	0.0018953393605124999	0.0
28	0.0	0.0	0.010319069851679165	0.005686018081537499	0.0
29	0.0	0.0	0.010319069851679165	0.0202169531788	0.0
30	0.0	0.0	0.010319069851679165	0.03769619394797083	0.0
31	0.0	0.0	0.010319069851679165	0.09392459497650832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTCG	35	8.861697E-4	26.428572	9
GTATCAA	700	0.0	24.842855	1
GGTATCA	240	0.0	23.125002	1
ACGGGAC	65	2.6773432E-6	22.76923	15
TTAACGG	90	3.814421E-9	22.61111	35
ACGTAGT	60	3.7203587E-5	21.583334	1
TAACGGC	95	7.1177055E-9	21.421053	36
TTTAACG	100	1.2849341E-8	20.35	34
GCACCGT	55	5.1369856E-4	20.181818	6
GCCGAGT	55	5.1369856E-4	20.181818	12
TTCGCCG	55	5.1369856E-4	20.181818	24
TACCCTC	125	4.110916E-10	19.24	5
CCAATAC	100	2.8661088E-7	18.5	3
AACGGCC	110	3.837522E-8	18.5	37
ATTACCC	145	1.546141E-10	17.862068	3
GCACCGC	115	6.3813786E-8	17.69565	10
ATGCCCG	105	4.782614E-7	17.619047	29
CTGCTCG	150	2.4920155E-10	17.266666	9
CGAGTTC	65	0.0015780885	17.076923	14
CAACCCA	130	1.3869794E-8	17.076923	17
>>END_MODULE