FastQCFastQC Report
Fri 10 Feb 2017
ERR1633073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389480
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9750.25033377837116155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.1810105782068399No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4090.10501181061928724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3960.10167402690767176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6450.025.240311
GGTATCA2200.025.2272721
TTCGCCG450.003821335320.55555524
GTCAACC555.134706E-420.18181815
AGCCGGT759.2400005E-619.73333431
TACATGC1457.2759576E-1219.137932
CCTATAC1750.019.0285723
ATAATAG500.007026736618.53
GTAATAC801.6122023E-518.53
CCTCATA500.007026736618.51
CCCTTAG500.007026736618.51
ACCCATC500.007026736618.58
GTACATG1750.017.971431
CATGCTA1451.546141E-1017.8620684
GCCGGTG953.5940157E-617.52631632
TATACAG953.5940157E-617.5263165
CGGTGCT852.7156371E-517.41176434
CGAACTA1603.274181E-1117.3437529
ATGCTAA1603.274181E-1117.343755
TTAGCCG752.0628478E-417.26666629
Produced by FastQC (version 0.11.5)