##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389480
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564098284892676	33.0	33.0	33.0	27.0	33.0
2	31.123497997329775	33.0	33.0	33.0	27.0	33.0
3	31.552218342405258	33.0	33.0	33.0	27.0	33.0
4	31.701820375885795	33.0	33.0	33.0	27.0	33.0
5	31.821590325562287	33.0	33.0	33.0	33.0	33.0
6	34.653953989935296	37.0	37.0	37.0	27.0	37.0
7	34.85941255006676	37.0	37.0	37.0	27.0	37.0
8	35.02505135051864	37.0	37.0	37.0	33.0	37.0
9	35.13128273595564	37.0	37.0	37.0	33.0	37.0
10	35.15632124884461	37.0	37.0	37.0	33.0	37.0
11	35.17594484954298	37.0	37.0	37.0	33.0	37.0
12	35.125133511348466	37.0	37.0	37.0	33.0	37.0
13	35.15561004416145	37.0	37.0	37.0	33.0	37.0
14	35.121141008524184	37.0	37.0	37.0	33.0	37.0
15	35.12873318270515	37.0	37.0	37.0	33.0	37.0
16	35.13923436376707	37.0	37.0	37.0	33.0	37.0
17	35.09809489575845	37.0	37.0	37.0	33.0	37.0
18	35.11701499435144	37.0	37.0	37.0	33.0	37.0
19	35.121079387901815	37.0	37.0	37.0	33.0	37.0
20	35.11801632946493	37.0	37.0	37.0	33.0	37.0
21	35.10018999691897	37.0	37.0	37.0	33.0	37.0
22	35.116275546883024	37.0	37.0	37.0	33.0	37.0
23	35.10071890726096	37.0	37.0	37.0	33.0	37.0
24	35.089719626168225	37.0	37.0	37.0	33.0	37.0
25	35.08222245044675	37.0	37.0	37.0	33.0	37.0
26	35.047976789565574	37.0	37.0	37.0	33.0	37.0
27	34.99697288692616	37.0	37.0	37.0	27.0	37.0
28	34.97074560953066	37.0	37.0	37.0	27.0	37.0
29	34.99996148711102	37.0	37.0	37.0	27.0	37.0
30	34.9682628119544	37.0	37.0	37.0	27.0	37.0
31	34.949504467495125	37.0	37.0	37.0	27.0	37.0
32	34.97561107117182	37.0	37.0	37.0	27.0	37.0
33	34.95618516997022	37.0	37.0	37.0	27.0	37.0
34	34.90213104652357	37.0	37.0	37.0	27.0	37.0
35	34.89575074458252	37.0	37.0	37.0	27.0	37.0
36	34.874306767998355	37.0	37.0	37.0	27.0	37.0
37	34.89260552531581	37.0	37.0	37.0	27.0	37.0
38	34.747008832289204	37.0	37.0	37.0	27.0	37.0
39	34.817341070144806	37.0	37.0	37.0	27.0	37.0
40	34.851019307795006	37.0	37.0	37.0	27.0	37.0
41	34.87349286227791	37.0	37.0	37.0	27.0	37.0
42	34.795006162062236	37.0	37.0	37.0	27.0	37.0
43	34.57653281298141	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	17.0
16	21.0
17	29.0
18	19.0
19	15.0
20	40.0
21	90.0
22	263.0
23	631.0
24	1313.0
25	2244.0
26	3690.0
27	5414.0
28	7390.0
29	9938.0
30	12974.0
31	16245.0
32	20453.0
33	26531.0
34	37674.0
35	67446.0
36	177040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76255520180754	20.644962514121392	13.439200985929958	25.15328129814111
2	16.078360891445005	22.859967135668068	37.679983567834036	23.38168840505289
3	17.813751668891857	26.805484235390775	30.10295778987368	25.277806305843686
4	12.868696723836912	18.10131457327719	38.84384307281504	30.186145630070865
5	14.15502721577488	36.912293314162476	35.25264455170997	13.680034918352677
6	29.827975762555205	39.75454452089966	17.34723220704529	13.070247509499847
7	26.881996508164736	31.93822532607579	23.51956454760193	17.660213618157545
8	24.430522748279756	36.19133203245353	20.550477559823356	18.82766765944336
9	25.928417377015506	15.518640238266407	20.189226661189277	38.363715723528806
10	14.941460408750128	28.177826846051147	33.91290952038616	22.96780322481257
11	34.37917222963952	23.50415939200986	24.068244839272875	18.048423539077746
12	23.137003183732155	25.436736161035228	30.355602341583648	21.07065831364897
13	27.966776214439765	22.112046831673	26.021618568347538	23.89955838553969
14	21.899712437095616	20.821608298243813	27.40063674643114	29.878042518229435
15	23.861045496559516	28.619441306357196	24.17916195953579	23.340351237547498
16	23.296703296703296	28.341891753106708	24.89909623087193	23.462308719318063
17	22.718496456814215	27.49152716442436	26.216237033994044	23.573739344767382
18	23.29464927595769	27.26430111944131	27.349799732977303	22.091249871623702
19	24.760449830543287	26.419071582622983	26.789565574612308	22.030913012221422
20	24.189432063263837	26.369261579541952	26.97776522542878	22.46354113176543
21	23.2753928314676	26.97776522542878	27.093817397555714	22.653024545547908
22	22.943411728458457	26.81190305022081	27.02988600184862	23.214799219472116
23	23.191177980897606	26.591609325254183	27.464054636951836	22.753158056896375
24	23.246636541029066	27.1777754955325	26.008267433501082	23.56732052993735
25	23.433809181472736	26.756957995275755	27.111533326486597	22.697699496764915
26	23.341635000513506	27.058899044880352	26.9823867721064	22.617079182499744
27	23.216596487624525	26.996508164732465	26.509962000616206	23.276933347026805
28	22.96087090479614	26.697904898839482	27.33696210331724	23.00426209304714
29	24.09006880969498	26.68506726917942	26.449881893807124	22.774982027318476
30	23.65975146348978	26.491989319092124	26.97571120468317	22.872548012734928
31	23.436633459997946	26.480692204991268	26.93077950087296	23.151894834137828
32	22.545701961589813	26.516637568039435	27.284841326897403	23.65281914347335
33	22.677416041902024	26.61369004826949	27.483054328848723	23.225839580979766
34	23.39709356064496	26.2280476532813	27.091763376810107	23.28309540926363
35	23.082571633973505	27.03168327000103	26.883537023723942	23.00220807230153
36	23.944746841943104	26.6160008216083	26.565163808154463	22.874088528294138
37	23.237906952860225	26.289411523056383	26.916144603060495	23.556536921022904
38	23.21813700318373	26.704580466262712	26.804970730204374	23.272311800349183
39	22.87511553866694	26.551555920714797	27.227842251206734	23.345486289411525
40	23.018640238266403	26.930522748279756	27.34107014480846	22.709766868645374
41	23.226609838759373	26.124319605628017	27.20499127041183	23.44407928520078
42	22.847899763787613	27.005494505494504	27.037588579644655	23.109017151073225
43	23.474889596384923	25.93355242887953	27.235544829002773	23.35601314573277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	10.0
2	12.0
3	58.5
4	105.0
5	105.0
6	127.5
7	150.0
8	137.0
9	124.0
10	172.0
11	220.0
12	220.0
13	344.5
14	469.0
15	886.5
16	1304.0
17	1514.5
18	1725.0
19	1725.0
20	1725.5
21	1726.0
22	2116.0
23	2506.0
24	3404.0
25	4302.0
26	4302.0
27	5663.0
28	7024.0
29	8902.5
30	10781.0
31	13153.0
32	15525.0
33	15525.0
34	17992.0
35	20459.0
36	22575.0
37	24691.0
38	26607.0
39	28523.0
40	28523.0
41	30080.5
42	31638.0
43	32020.5
44	32403.0
45	31867.0
46	31331.0
47	31331.0
48	30473.0
49	29615.0
50	29155.0
51	28695.0
52	27222.0
53	25749.0
54	25749.0
55	24674.5
56	23600.0
57	20823.0
58	18046.0
59	16523.0
60	15000.0
61	15000.0
62	13220.0
63	11440.0
64	9634.0
65	7828.0
66	6663.5
67	5499.0
68	5499.0
69	4477.0
70	3455.0
71	2848.0
72	2241.0
73	1830.5
74	1420.0
75	1420.0
76	1093.5
77	767.0
78	617.5
79	468.0
80	372.5
81	277.0
82	277.0
83	212.5
84	148.0
85	132.5
86	117.0
87	87.5
88	58.0
89	58.0
90	41.0
91	24.0
92	15.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	389480.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.49776441397908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.117349766624	61.93456579279009
2	6.247735144074429	8.684072503276449
3	1.7510046846641143	3.6507273318768103
4	0.7835487780186116	2.178195535263946
5	0.4604259813484395	1.5999288190914487
6	0.30174901349625605	1.258252911126804
7	0.20769813419458596	1.0104189199634843
8	0.14404420757223127	0.8008600322442584
9	0.1158450796626818	0.7245876649424129
>10	0.763295928277833	10.141364846339444
>50	0.07388703505854534	3.5908760775549284
>100	0.03267366374141521	3.9926433992564028
>500	7.425832668503456E-4	0.4335061662735576
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	975	0.25033377837116155	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	705	0.1810105782068399	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	409	0.10501181061928724	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	396	0.10167402690767176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	2.5675259320119136E-4	0.0	0.0
6	0.0	0.0	0.0010270103728047654	2.5675259320119136E-4	0.0
7	0.0	0.0	0.002054020745609531	2.5675259320119136E-4	0.0
8	0.0	0.0	0.003081031118414296	2.5675259320119136E-4	0.0
9	0.0	0.0	0.004621546677621444	5.135051864023827E-4	0.0
10	0.0	0.0	0.004878299270822635	5.135051864023827E-4	0.0
11	0.0	0.0	0.007189072609633357	7.70257779603574E-4	0.0
12	0.0	0.0	0.008216082982438123	0.0010270103728047654	0.0
13	0.0	0.0	0.00949984594844408	0.0010270103728047654	0.0
14	0.0	0.0	0.011040361507651227	0.0010270103728047654	0.0
15	0.0	0.0	0.01155386669405361	0.0010270103728047654	0.0
16	2.5675259320119136E-4	0.0	0.012580877066858375	0.0010270103728047654	0.0
17	2.5675259320119136E-4	0.0	0.013094382253260758	0.0010270103728047654	0.0
18	2.5675259320119136E-4	0.0	0.01360788743966314	0.0010270103728047654	0.0
19	2.5675259320119136E-4	0.0	0.013864640032864332	0.0010270103728047654	0.0
20	2.5675259320119136E-4	0.0	0.014378145219266714	0.0010270103728047654	0.0
21	2.5675259320119136E-4	0.0	0.01566190818527267	0.0010270103728047654	0.0
22	2.5675259320119136E-4	0.0	0.015918660778473863	0.0010270103728047654	0.0
23	2.5675259320119136E-4	0.0	0.015918660778473863	0.0012837629660059566	0.0
24	2.5675259320119136E-4	0.0	0.015918660778473863	0.001540515559207148	0.0
25	2.5675259320119136E-4	0.0	0.015918660778473863	0.001540515559207148	0.0
26	2.5675259320119136E-4	0.0	0.015918660778473863	0.0017972681524083393	0.0
27	2.5675259320119136E-4	0.0	0.015918660778473863	0.002567525932011913	0.0
28	2.5675259320119136E-4	0.0	0.015918660778473863	0.006418814830029783	0.0
29	2.5675259320119136E-4	0.0	0.015918660778473863	0.01566190818527267	0.0
30	2.5675259320119136E-4	0.0	0.015918660778473863	0.029783300811338193	0.0
31	2.5675259320119136E-4	0.0	0.015918660778473863	0.07471500462154668	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	645	0.0	25.24031	1
GGTATCA	220	0.0	25.227272	1
TTCGCCG	45	0.0038213353	20.555555	24
GTCAACC	55	5.134706E-4	20.181818	15
AGCCGGT	75	9.2400005E-6	19.733334	31
TACATGC	145	7.2759576E-12	19.13793	2
CCTATAC	175	0.0	19.028572	3
ATAATAG	50	0.0070267366	18.5	3
GTAATAC	80	1.6122023E-5	18.5	3
CCTCATA	50	0.0070267366	18.5	1
CCCTTAG	50	0.0070267366	18.5	1
ACCCATC	50	0.0070267366	18.5	8
GTACATG	175	0.0	17.97143	1
CATGCTA	145	1.546141E-10	17.862068	4
GCCGGTG	95	3.5940157E-6	17.526316	32
TATACAG	95	3.5940157E-6	17.526316	5
CGGTGCT	85	2.7156371E-5	17.411764	34
CGAACTA	160	3.274181E-11	17.34375	29
ATGCTAA	160	3.274181E-11	17.34375	5
TTAGCCG	75	2.0628478E-4	17.266666	29
>>END_MODULE