FastQCFastQC Report
Fri 10 Feb 2017
ERR1633072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528014
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20440.3871109478157776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.28275765415310955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7590.14374618854803092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.026.5641041
TAGACCG358.8630203E-426.428575
GACCTAT358.8630203E-426.428576
GTATCAA10900.023.082571
TTAACGG957.1231625E-921.42105335
CTTAACG450.003823109720.55555717
GGCTAGG450.003823109720.55555737
AACGGCC1001.2858436E-820.3537
TAAGCAG951.6718332E-719.4736845
TTTAACG1052.2524546E-819.38095334
TTACTCA500.00702996918.54
CGCGATA1002.8676186E-718.534
AAGCGGA701.2176139E-418.523
TACGCCC902.1477354E-618.54
TTGACTA500.00702996918.55
CGGGATT500.00702996918.516
CATACCC609.2278013E-418.524
GCTACCG500.00702996918.57
GATCTAC500.00702996918.511
GTATTAG1750.017.971431
Produced by FastQC (version 0.11.5)