FastQCFastQC Report
Fri 10 Feb 2017
ERR1633071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543074
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18450.3397327067766087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.26663033030489397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6370.11729524889794024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA303.597E-430.83333417
GGTATCA3650.028.8904111
GTATCAA10000.022.0151
CGGGATA450.003823248720.55555517
CTACACT951.6720514E-719.4736844
GCCGCTC1355.638867E-1119.18518627
CGAACTA902.1479646E-618.524
GACCGTG500.007030220218.57
TGTTATA500.007030220218.52
TTAACGA500.007030220218.520
GCGTCAA609.228264E-418.526
AGCTTCG1451.546141E-1017.86206821
GCCGGCA1451.546141E-1017.86206815
CGAGTCG953.5993035E-617.52631621
AACGGCC852.7188353E-517.41176437
CTGCTCG2050.017.1463419
GCTCGGA650.001578483717.07692311
GTATTAG1851.8189894E-1217.01
TTCGCCG1553.9835868E-1016.70967724
CCGTCCG1005.86631E-616.65000228
Produced by FastQC (version 0.11.5)