FastQCFastQC Report
Fri 10 Feb 2017
ERR1633070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485463
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12680.2611939529892082No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9060.1866259632556961No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8480.17467860578458091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.17323668333117045No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7260.14954795731085582No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6900.14213235612188777No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6560.13512873277675128No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6540.1347167549329197No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6480.13348082140142503No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6260.1289490651192779No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5830.12009154147689938No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5550.11432385166325755No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5470.11267594028793132No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5170.10649627263045794No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5160.10629028370854215No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4980.10258248311405813No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4920.10134654958256345No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4890.10072858281681611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT250.00549325529.610
GGTATCA3900.027.987181
TAAGCTG451.3216745E-424.6666665
ATACTGG1750.021.1428596
CCGTTAA1400.021.14285716
GTATCAA9750.020.682051
CTAGTCC450.003822672620.5555553
GTATAAC450.003822672620.5555551
CGTTAAA1450.020.41379417
GCCGTTA1550.020.29032315
CTGTACG555.137214E-420.18181826
GTATACG555.137214E-420.1818181
TGTACGA555.137214E-420.18181827
GTACGAA555.137214E-420.18181828
TTTAAGC759.247702E-619.7333343
CGGCCGT1551.8189894E-1219.09677513
GGCCGTT1551.8189894E-1219.09677514
GTGTCAC1650.019.06060627
TCTAATA1750.019.0285722
TTAACGG1850.019.035
Produced by FastQC (version 0.11.5)