##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485463
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7674508664924	33.0	33.0	33.0	27.0	33.0
2	31.296055518134235	33.0	33.0	33.0	27.0	33.0
3	31.717817835756794	33.0	33.0	33.0	27.0	33.0
4	31.835824769343905	33.0	33.0	33.0	27.0	33.0
5	31.95966942897811	33.0	33.0	33.0	33.0	33.0
6	34.860044946782764	37.0	37.0	37.0	27.0	37.0
7	34.98040633374737	37.0	37.0	37.0	27.0	37.0
8	35.18126201172901	37.0	37.0	37.0	33.0	37.0
9	35.218471850583875	37.0	37.0	37.0	33.0	37.0
10	35.25443957623959	37.0	37.0	37.0	33.0	37.0
11	35.297666351503615	37.0	37.0	37.0	33.0	37.0
12	35.231997083196866	37.0	37.0	37.0	33.0	37.0
13	35.273670701989644	37.0	37.0	37.0	33.0	37.0
14	35.205690237979	37.0	37.0	37.0	33.0	37.0
15	35.26894943589934	37.0	37.0	37.0	33.0	37.0
16	35.246053767228396	37.0	37.0	37.0	33.0	37.0
17	35.24735767710413	37.0	37.0	37.0	33.0	37.0
18	35.22195512325347	37.0	37.0	37.0	33.0	37.0
19	35.26576484716652	37.0	37.0	37.0	33.0	37.0
20	35.17030958075075	37.0	37.0	37.0	33.0	37.0
21	35.243126664647974	37.0	37.0	37.0	33.0	37.0
22	35.23688108053549	37.0	37.0	37.0	33.0	37.0
23	35.25712979155981	37.0	37.0	37.0	33.0	37.0
24	35.24227593040046	37.0	37.0	37.0	33.0	37.0
25	35.216535554717865	37.0	37.0	37.0	33.0	37.0
26	35.1970016252526	37.0	37.0	37.0	33.0	37.0
27	35.117870980898644	37.0	37.0	37.0	33.0	37.0
28	35.127021008810146	37.0	37.0	37.0	33.0	37.0
29	35.16839800355537	37.0	37.0	37.0	33.0	37.0
30	35.130815324751836	37.0	37.0	37.0	33.0	37.0
31	35.108605599190874	37.0	37.0	37.0	33.0	37.0
32	35.14729237861588	37.0	37.0	37.0	33.0	37.0
33	35.14402745420351	37.0	37.0	37.0	33.0	37.0
34	35.0981784399635	37.0	37.0	37.0	33.0	37.0
35	35.079703293556875	37.0	37.0	37.0	33.0	37.0
36	35.05988715926858	37.0	37.0	37.0	27.0	37.0
37	35.1015978560673	37.0	37.0	37.0	33.0	37.0
38	34.945993824452124	37.0	37.0	37.0	27.0	37.0
39	35.015173143988314	37.0	37.0	37.0	27.0	37.0
40	35.066464385545345	37.0	37.0	37.0	27.0	37.0
41	35.08600449467828	37.0	37.0	37.0	33.0	37.0
42	34.96675544789201	37.0	37.0	37.0	27.0	37.0
43	34.79476293764921	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	15.0
16	24.0
17	29.0
18	17.0
19	22.0
20	40.0
21	81.0
22	263.0
23	606.0
24	1227.0
25	2317.0
26	3909.0
27	5735.0
28	8328.0
29	11108.0
30	14710.0
31	19120.0
32	24114.0
33	31788.0
34	45426.0
35	83541.0
36	233040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40513901162395	20.88233294813405	13.72607181185796	24.98645622838404
2	15.792552676517058	22.709866663370846	38.460603588739	23.0369770713731
3	17.1833898772924	27.547722483484836	29.92874843190933	25.340139207313428
4	12.309074017999313	18.06914224153025	39.944135804376444	29.677647936093997
5	13.474559338198791	37.117555817848114	36.32635236876961	13.081532475183485
6	29.14001684989381	40.77427115969704	17.190393500637533	12.89531848977162
7	26.670827642889368	31.907684004754223	24.222649305920328	17.19883904643608
8	23.706441067599386	36.69033479379479	20.675726059452522	18.927498079153303
9	25.628317709073606	15.639502907533634	20.459437691440954	38.27274169195181
10	14.875489996148005	28.092563181952073	34.60140113664687	22.430545685253048
11	33.6763048883231	23.96681106490093	24.206582170010897	18.150301876765067
12	22.83840375064629	26.24484255236753	30.00949608930032	20.907257607685857
13	27.193215548867784	22.655485587985076	26.273063034669995	23.878235828477145
14	21.784976403968994	20.808382925166285	27.728580756926892	29.678059913937826
15	23.638258734445262	28.99026290366104	24.78726494089148	22.584213421002218
16	23.048512451000384	28.648115304358935	25.347966786346234	22.955405458294454
17	22.323019468013012	27.83981477476141	26.375027551018306	23.46213820620727
18	22.60028055691165	27.911292930666185	27.78687562182906	21.701550890593104
19	24.523393131917366	26.454127297033963	27.384373268405625	21.638106302643042
20	23.758556264844078	26.81048813194826	27.593246035228226	21.837709567979434
21	22.475039292386857	27.56626148645726	27.69253269559163	22.266166525564255
22	22.480806982200498	27.201867083588244	27.426806986320273	22.890518947890985
23	22.520562844130243	27.049641270292486	28.031796449986917	22.397999435590354
24	22.79885387763846	27.948988903376776	26.032879951716197	23.219277267268566
25	23.011022467211713	27.149545897421635	27.596953835822706	22.24247779954394
26	22.884545269155424	27.58521246727351	27.28755847510521	22.242683788465854
27	22.751270436675917	27.382931345952215	26.561035547508254	23.304762669863617
28	22.399441358043763	27.128123049542396	28.001310089543384	22.471125502870457
29	23.810465473166854	27.078067741516858	27.0302783116324	22.081188473683884
30	23.187554973293537	26.857865583988893	27.408061994425942	22.54651744829163
31	22.902054327518265	27.066326372967666	27.369748054949603	22.661871244564466
32	21.98581560283688	26.843652348376708	27.62476234028134	23.545769708505077
33	22.148752840072262	26.853333827706745	28.23304762669864	22.764865705522357
34	23.033681248622447	26.531373142752386	28.06331275504003	22.371632853585137
35	22.68864980441352	27.55163627300124	27.140894362701175	22.61881955988407
36	23.406727186211924	27.266547605069803	26.84159245915755	22.48513274956073
37	22.541779703087567	26.63889935999242	27.322370602908975	23.49695033401104
38	22.80812337912467	27.230293554812622	27.159021387829764	22.802561678232944
39	22.210137538803163	27.25686612573976	27.73084663506797	22.802149700389112
40	22.509233453424876	27.61446289418555	27.800676879597415	22.07562677279216
41	22.553727060558685	26.600791409438003	27.610137126825318	23.235344403177997
42	22.149576795759923	27.663694246523423	27.40188232676847	22.784846630948188
43	23.331747218634582	26.03720571907643	27.41073985041084	23.220307211878144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	10.5
2	16.0
3	76.0
4	136.0
5	136.0
6	166.5
7	197.0
8	180.0
9	163.0
10	229.5
11	296.0
12	296.0
13	477.0
14	658.0
15	1175.5
16	1693.0
17	2028.5
18	2364.0
19	2364.0
20	2310.0
21	2256.0
22	2717.5
23	3179.0
24	4368.5
25	5558.0
26	5558.0
27	7293.0
28	9028.0
29	11345.0
30	13662.0
31	16817.5
32	19973.0
33	19973.0
34	23680.0
35	27387.0
36	30533.5
37	33680.0
38	36818.5
39	39957.0
40	39957.0
41	41537.0
42	43117.0
43	43633.0
44	44149.0
45	42114.0
46	40079.0
47	40079.0
48	37687.0
49	35295.0
50	34906.5
51	34518.0
52	31750.5
53	28983.0
54	28983.0
55	28432.0
56	27881.0
57	23611.0
58	19341.0
59	17941.5
60	16542.0
61	16542.0
62	14537.5
63	12533.0
64	10344.0
65	8155.0
66	6768.5
67	5382.0
68	5382.0
69	4502.5
70	3623.0
71	2983.0
72	2343.0
73	1888.5
74	1434.0
75	1434.0
76	1112.0
77	790.0
78	628.5
79	467.0
80	373.0
81	279.0
82	279.0
83	217.5
84	156.0
85	127.0
86	98.0
87	78.5
88	59.0
89	59.0
90	41.5
91	24.0
92	15.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	485463.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.26054036679308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.22138863932385	55.54971868959957
2	6.039929190413565	7.520985103446312
3	1.6117493284474018	3.010451523748533
4	0.7401618432135129	1.8433150526941955
5	0.44327496601010397	1.3799269457430456
6	0.29011771492175575	1.0837731420604644
7	0.2240823736998971	0.9766042771260481
8	0.16244590784204874	0.809117600209618
9	0.13787126567690244	0.7725545551887875
>10	0.931512103721092	11.231677775280746
>50	0.1166848486041974	5.147082089561387
>100	0.07579528614257061	8.484778949093998
>500	0.004654096517526265	1.9275685796192714
>1k	3.3243546553759037E-4	0.26244571662807215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.2611939529892082	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	906	0.1866259632556961	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	848	0.17467860578458091	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.17323668333117045	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	726	0.14954795731085582	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	690	0.14213235612188777	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	656	0.13512873277675128	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	654	0.1347167549329197	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	648	0.13348082140142503	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	626	0.1289490651192779	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	583	0.12009154147689938	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	555	0.11432385166325755	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	547	0.11267594028793132	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	517	0.10649627263045794	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	516	0.10629028370854215	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	498	0.10258248311405813	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	492	0.10134654958256345	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	489	0.10072858281681611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.119778438315587E-4	0.0	0.0
2	0.0	0.0	4.119778438315587E-4	0.0	0.0
3	0.0	0.0	4.119778438315587E-4	0.0	0.0
4	0.0	0.0	6.179667657473381E-4	2.0598892191577936E-4	0.0
5	0.0	0.0	6.179667657473381E-4	2.0598892191577936E-4	0.0
6	0.0	0.0	8.239556876631174E-4	2.0598892191577936E-4	0.0
7	0.0	0.0	0.002265878141073573	2.0598892191577936E-4	0.0
8	0.0	0.0	0.0028838449068209113	2.0598892191577936E-4	0.0
9	0.0	0.0	0.0030898338287366905	4.119778438315587E-4	0.0
10	0.0	0.0	0.0032958227506524698	4.119778438315587E-4	0.0
11	0.0	0.0	0.006385656579389161	4.119778438315587E-4	0.0
12	0.0	0.0	0.006797634423220719	4.119778438315587E-4	0.0
13	0.0	0.0	0.007003623345136499	4.119778438315587E-4	0.0
14	0.0	0.0	0.007415601188968057	6.179667657473381E-4	0.0
15	0.0	0.0	0.007621590110883837	6.179667657473381E-4	0.0
16	0.0	0.0	0.008239556876631174	6.179667657473381E-4	0.0
17	0.0	0.0	0.008651534720462734	6.179667657473381E-4	0.0
18	0.0	0.0	0.009063512564294293	6.179667657473381E-4	0.0
19	0.0	2.0598892191577936E-4	0.00947549040812585	8.239556876631174E-4	0.0
20	0.0	2.0598892191577936E-4	0.00947549040812585	8.239556876631174E-4	0.0
21	0.0	2.0598892191577936E-4	0.00988746825195741	0.0012359335314946762	0.0
22	0.0	2.0598892191577936E-4	0.00988746825195741	0.0012359335314946762	0.0
23	0.0	2.0598892191577936E-4	0.010093457173873189	0.0012359335314946762	0.0
24	0.0	2.0598892191577936E-4	0.010093457173873189	0.0018539002972420143	0.0
25	0.0	2.0598892191577936E-4	0.010093457173873189	0.0020598892191577936	0.0
26	0.0	2.0598892191577936E-4	0.010093457173873189	0.002265878141073573	0.0
27	0.0	2.0598892191577936E-4	0.010093457173873189	0.0035018116725682494	0.0
28	0.0	2.0598892191577936E-4	0.010093457173873189	0.007209612267052278	0.0
29	0.0	2.0598892191577936E-4	0.010093457173873189	0.019362958660083262	0.0
30	0.0	2.0598892191577936E-4	0.010093457173873189	0.03934388408591386	0.0
31	0.0	2.0598892191577936E-4	0.010093457173873189	0.09537287084700585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGT	25	0.005493255	29.6	10
GGTATCA	390	0.0	27.98718	1
TAAGCTG	45	1.3216745E-4	24.666666	5
ATACTGG	175	0.0	21.142859	6
CCGTTAA	140	0.0	21.142857	16
GTATCAA	975	0.0	20.68205	1
CTAGTCC	45	0.0038226726	20.555555	3
GTATAAC	45	0.0038226726	20.555555	1
CGTTAAA	145	0.0	20.413794	17
GCCGTTA	155	0.0	20.290323	15
CTGTACG	55	5.137214E-4	20.181818	26
GTATACG	55	5.137214E-4	20.181818	1
TGTACGA	55	5.137214E-4	20.181818	27
GTACGAA	55	5.137214E-4	20.181818	28
TTTAAGC	75	9.247702E-6	19.733334	3
CGGCCGT	155	1.8189894E-12	19.096775	13
GGCCGTT	155	1.8189894E-12	19.096775	14
GTGTCAC	165	0.0	19.060606	27
TCTAATA	175	0.0	19.028572	2
TTAACGG	185	0.0	19.0	35
>>END_MODULE