FastQCFastQC Report
Fri 10 Feb 2017
ERR1633069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400553
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18250.4556201052045547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.3400299086512896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7060.17625632562981677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC352.3844526E-531.71428727
CGTGCGT250.005492120529.614
TACCGGC250.005492120529.67
GGTATCA2450.028.6938761
TCTTCGA358.859267E-426.42857211
TTCGCCG451.3211105E-424.66666624
GTATCAA8100.023.9814821
ATCTCGC551.8986493E-523.54545411
GTCCGAC400.001929002923.12500211
TGTACCG400.001929002923.1250025
CTGTACC502.6978445E-422.24
TCTCGCC502.6978445E-422.212
CGGTTCG603.7184884E-521.58333422
AGCTTCG603.7184884E-521.58333421
GCTTCGC603.7184884E-521.58333422
CCCATAG705.088239E-621.1428591
TAATGGA450.003821522520.5555554
CGGCCTT555.135058E-420.18181824
GCCGGCA555.135058E-420.18181815
CCGCTCT555.135058E-420.18181828
Produced by FastQC (version 0.11.5)