##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400553
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73740054374827	33.0	33.0	33.0	27.0	33.0
2	31.237661433068784	33.0	33.0	33.0	27.0	33.0
3	31.69277473892344	33.0	33.0	33.0	27.0	33.0
4	31.806589889477795	33.0	33.0	33.0	27.0	33.0
5	31.921838058883594	33.0	33.0	33.0	33.0	33.0
6	34.82304963388116	37.0	37.0	37.0	27.0	37.0
7	34.99071034295087	37.0	37.0	37.0	33.0	37.0
8	35.155080601069024	37.0	37.0	37.0	33.0	37.0
9	35.22790991454315	37.0	37.0	37.0	33.0	37.0
10	35.254755300796646	37.0	37.0	37.0	33.0	37.0
11	35.289117794648895	37.0	37.0	37.0	33.0	37.0
12	35.264793922402276	37.0	37.0	37.0	33.0	37.0
13	35.27857986333893	37.0	37.0	37.0	33.0	37.0
14	35.23191937146895	37.0	37.0	37.0	33.0	37.0
15	35.25744907665153	37.0	37.0	37.0	33.0	37.0
16	35.277838388428	37.0	37.0	37.0	33.0	37.0
17	35.229794808677006	37.0	37.0	37.0	33.0	37.0
18	35.23631080031856	37.0	37.0	37.0	33.0	37.0
19	35.23561925637806	37.0	37.0	37.0	33.0	37.0
20	35.222896845111634	37.0	37.0	37.0	33.0	37.0
21	35.24264704046656	37.0	37.0	37.0	33.0	37.0
22	35.23728695079053	37.0	37.0	37.0	33.0	37.0
23	35.224931532156795	37.0	37.0	37.0	33.0	37.0
24	35.22531849717765	37.0	37.0	37.0	33.0	37.0
25	35.20588536348498	37.0	37.0	37.0	33.0	37.0
26	35.17747214475987	37.0	37.0	37.0	33.0	37.0
27	35.12621301051296	37.0	37.0	37.0	33.0	37.0
28	35.102470834071895	37.0	37.0	37.0	33.0	37.0
29	35.133972283318315	37.0	37.0	37.0	33.0	37.0
30	35.09226244716679	37.0	37.0	37.0	33.0	37.0
31	35.091401137926816	37.0	37.0	37.0	33.0	37.0
32	35.11432194990426	37.0	37.0	37.0	33.0	37.0
33	35.08228873582272	37.0	37.0	37.0	33.0	37.0
34	35.0055648066548	37.0	37.0	37.0	27.0	37.0
35	35.006720708620335	37.0	37.0	37.0	27.0	37.0
36	34.97533909370296	37.0	37.0	37.0	27.0	37.0
37	35.01721370205691	37.0	37.0	37.0	27.0	37.0
38	34.861411598465125	37.0	37.0	37.0	27.0	37.0
39	34.91953374459809	37.0	37.0	37.0	27.0	37.0
40	34.96051708512981	37.0	37.0	37.0	27.0	37.0
41	34.97151438136776	37.0	37.0	37.0	27.0	37.0
42	34.88350605288189	37.0	37.0	37.0	27.0	37.0
43	34.67826729546402	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	10.0
16	23.0
17	17.0
18	22.0
19	20.0
20	31.0
21	80.0
22	205.0
23	567.0
24	1112.0
25	2020.0
26	3225.0
27	4969.0
28	7101.0
29	9569.0
30	12524.0
31	15846.0
32	20155.0
33	26445.0
34	37187.0
35	67635.0
36	191786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.410632300844085	20.956277945740016	14.194376274800089	24.438713478615814
2	15.404453343252952	24.045756741305148	38.64532284117208	21.904467074269824
3	17.44088797237819	27.414100006740682	31.437787259114273	23.707224761766856
4	12.425321992345582	18.387579171794993	40.08682995758364	29.10026887827578
5	13.22621475809693	37.62772966373988	35.75232241426228	13.393733163900906
6	28.136351494059465	40.90345097902151	17.802887508020163	13.15731001889887
7	25.158218762560757	33.27649524532334	23.984091992819927	17.581193999295973
8	23.794603960025267	36.347499581828124	21.433867677935254	18.42402878021136
9	25.850761322471683	15.632887532985649	20.67991002439128	37.83644112015139
10	14.224334857060114	28.760238969624496	34.80588086969764	22.209545303617748
11	32.698044952852676	24.65266768692283	24.646675970470824	18.002611389753664
12	22.041277933257273	26.35406550444011	31.35639977730787	20.248256784994744
13	27.72442098798411	22.90807957998068	26.930768212945605	22.43673121908961
14	21.813842362933244	21.767905870134538	28.63990533088006	27.77834643605216
15	23.178455784877407	29.308481024982964	25.19741457435096	22.31564861578867
16	22.037782765326934	29.445042229118247	25.71469942804073	22.802475577514087
17	21.65905635458978	28.176046615553997	27.16769066765197	22.997206362204253
18	22.321640332240676	27.617069401552353	28.498351029701436	21.56293923650553
19	23.872995583605665	27.023390163099513	28.646396357036398	20.457217896258424
20	23.97410579873325	26.68785404178723	28.29413336062893	21.04390679885059
21	22.331127216623017	27.867473218275734	27.782590568538996	22.018808996562253
22	21.786879638899222	27.729164430175285	28.15807146619798	22.325884464727512
23	22.02055658052742	27.488247497834244	28.49984895881444	21.991346962823897
24	22.225023904452097	27.702701015845594	27.946863461264805	22.12541161843751
25	22.44946361655012	27.427831023609862	28.44068075885089	21.682024600989134
26	22.29767346643266	27.617319056404522	28.042231614792552	22.042775862370274
27	22.399532646116743	27.510716434529265	27.77584988753049	22.313901031823505
28	21.682024600989134	27.611327339952517	28.641153605140897	22.06549445391746
29	22.511627674739675	27.602589420126677	27.94811173552564	21.937671169608016
30	22.226771488417263	27.900926968466095	28.196767968283847	21.675533574832794
31	22.488160118635985	27.50347644381642	28.130359777607456	21.87800365994013
32	21.50701654962015	27.46602821599139	28.408724937773528	22.61823029661493
33	21.680526671876134	27.427581368757693	28.932251162767475	21.959640796598702
34	21.997837988980233	27.040366693046863	28.329834004488795	22.63196131348411
35	22.147381245428196	27.80705674405135	28.130609432459625	21.91495257806083
36	22.25373421245129	27.601840455570176	28.15807146619798	21.986353865780558
37	22.540088327886696	27.09529076052358	27.940871744812796	22.42374916677693
38	22.504886993731166	27.601091491013673	27.986059273055	21.907962242200156
39	22.12940609607218	27.258814688692883	28.33382848212346	22.277950733111474
40	22.010820041292913	27.34494561269046	28.509585498048946	22.134648847967682
41	22.06923927669996	27.203391311511837	28.47962691578892	22.247742495999283
42	22.000833847206238	27.30050704900475	28.31560367791528	22.383055425873728
43	21.950653221920692	27.029132224699353	28.52606271829196	22.49415183508799
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	23.0
2	30.0
3	127.0
4	224.0
5	224.0
6	265.5
7	307.0
8	287.5
9	268.0
10	368.5
11	469.0
12	469.0
13	734.5
14	1000.0
15	1870.5
16	2741.0
17	3144.0
18	3547.0
19	3547.0
20	3538.0
21	3529.0
22	4154.5
23	4780.0
24	6218.5
25	7657.0
26	7657.0
27	9313.5
28	10970.0
29	13651.0
30	16332.0
31	18101.0
32	19870.0
33	19870.0
34	21574.5
35	23279.0
36	24764.5
37	26250.0
38	27533.0
39	28816.0
40	28816.0
41	29725.0
42	30634.0
43	29925.5
44	29217.0
45	28900.0
46	28583.0
47	28583.0
48	27971.0
49	27359.0
50	27402.0
51	27445.0
52	27371.5
53	27298.0
54	27298.0
55	24650.5
56	22003.0
57	19716.5
58	17430.0
59	15211.0
60	12992.0
61	12992.0
62	11373.5
63	9755.0
64	8043.0
65	6331.0
66	5291.0
67	4251.0
68	4251.0
69	3488.5
70	2726.0
71	2318.5
72	1911.0
73	1521.0
74	1131.0
75	1131.0
76	858.5
77	586.0
78	451.0
79	316.0
80	242.5
81	169.0
82	169.0
83	144.0
84	119.0
85	118.0
86	117.0
87	86.5
88	56.0
89	56.0
90	45.0
91	34.0
92	19.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	400553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.6918849371811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58279458812738	62.22302903135958
2	5.232845493643266	7.189080410867797
3	1.3470924918768759	2.776029673552408
4	0.6451706114456913	1.7727194162511284
5	0.3945621998789117	1.3551610617321628
6	0.26957581943783043	1.1110602702401855
7	0.21179131459197886	1.0183841228852577
8	0.1435087430039382	0.7886308849524813
9	0.11866817682199829	0.733638667316559
>10	0.9369856075099217	12.69181908427294
>50	0.08592551284850379	4.173363255198529
>100	0.02998251937325548	3.1892974608624303
>500	3.656404801616522E-4	0.17732272856897743
>1k	7.312809603233044E-4	0.8004639319395626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1825	0.4556201052045547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1362	0.3400299086512896	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.17625632562981677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	2.496548521668793E-4	0.0	0.0
6	0.0	0.0	2.496548521668793E-4	0.0	0.0
7	0.0	0.0	0.002246893669501914	2.496548521668793E-4	0.0
8	0.0	0.0	0.0024965485216687927	2.496548521668793E-4	0.0
9	0.0	0.0	0.0029958582260025514	0.0012482742608343964	0.0
10	7.489645565006379E-4	0.0	0.00349516793033631	0.0014979291130012757	0.0
11	7.489645565006379E-4	0.0	0.005242751895504465	0.0014979291130012757	0.0
12	7.489645565006379E-4	0.0	0.006241371304171983	0.001747583965168155	0.0
13	7.489645565006379E-4	0.0	0.0067406810085057405	0.001747583965168155	0.0
14	7.489645565006379E-4	0.0	0.008238610121507017	0.001747583965168155	0.0
15	7.489645565006379E-4	0.0	0.008987574678007655	0.001747583965168155	0.0
16	7.489645565006379E-4	0.0	0.01073515864317581	0.001747583965168155	0.0
17	7.489645565006379E-4	0.0	0.011234468347509567	0.001747583965168155	0.0
18	7.489645565006379E-4	0.0	0.011983432904010206	0.001747583965168155	0.0
19	7.489645565006379E-4	0.0	0.011983432904010206	0.001747583965168155	0.0
20	7.489645565006379E-4	0.0	0.011983432904010206	0.0019972388173350344	0.0
21	7.489645565006379E-4	0.0	0.012982052312677723	0.002246893669501914	0.0
22	7.489645565006379E-4	0.0	0.013231707164844602	0.0024965485216687927	0.0
23	7.489645565006379E-4	0.0	0.013231707164844602	0.002746203373835672	0.0
24	7.489645565006379E-4	0.0	0.013481362017011481	0.00349516793033631	0.0
25	7.489645565006379E-4	0.0	0.013481362017011481	0.00349516793033631	0.0
26	7.489645565006379E-4	0.0	0.013481362017011481	0.0037448227825031895	0.0
27	7.489645565006379E-4	0.0	0.013481362017011481	0.004244132486836948	0.0
28	7.489645565006379E-4	0.0	0.013481362017011481	0.012233087756177085	0.0
29	7.489645565006379E-4	0.0	0.013481362017011481	0.024216520660187292	0.0
30	7.489645565006379E-4	0.0	0.013481362017011481	0.04368959912920387	0.0
31	7.489645565006379E-4	0.0	0.013481362017011481	0.09911297631025108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	35	2.3844526E-5	31.714287	27
CGTGCGT	25	0.0054921205	29.6	14
TACCGGC	25	0.0054921205	29.6	7
GGTATCA	245	0.0	28.693876	1
TCTTCGA	35	8.859267E-4	26.428572	11
TTCGCCG	45	1.3211105E-4	24.666666	24
GTATCAA	810	0.0	23.981482	1
ATCTCGC	55	1.8986493E-5	23.545454	11
GTCCGAC	40	0.0019290029	23.125002	11
TGTACCG	40	0.0019290029	23.125002	5
CTGTACC	50	2.6978445E-4	22.2	4
TCTCGCC	50	2.6978445E-4	22.2	12
CGGTTCG	60	3.7184884E-5	21.583334	22
AGCTTCG	60	3.7184884E-5	21.583334	21
GCTTCGC	60	3.7184884E-5	21.583334	22
CCCATAG	70	5.088239E-6	21.142859	1
TAATGGA	45	0.0038215225	20.555555	4
CGGCCTT	55	5.135058E-4	20.181818	24
GCCGGCA	55	5.135058E-4	20.181818	15
CCGCTCT	55	5.135058E-4	20.181818	28
>>END_MODULE