FastQCFastQC Report
Fri 10 Feb 2017
ERR1633068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658119
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32500.4938316626628315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.35525490070944615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10990.16699107608198516No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6960.10575594991179406No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6820.10362867505724649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG358.86535E-426.4285725
GGTATCA4650.025.0645161
GTATCAA13300.024.75941
TCTAAGC701.9203253E-723.7857153
ACGATCG400.001930315323.12516
GCACCGC1450.021.68965510
TTAACGG1450.021.68965535
AACGATT705.0964554E-621.14285922
CGCCTAT450.003824097320.55555534
AACGGCC1450.020.41379437
TAACGGC1500.019.73333236
GGACCGT759.255909E-619.7333326
ACTAGTG609.231096E-418.58
CGCCGTT609.231096E-418.525
TCTAATA2100.017.619052
GTTTAAC1900.017.52631433
CTCTAAT1800.017.4722211
GTGACAC2050.017.14634324
TAATACT2700.017.129634
CCGATGT650.001578963717.07692314
Produced by FastQC (version 0.11.5)