##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633068.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658119
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.737438062113387	33.0	33.0	33.0	27.0	33.0
2	31.25791080336535	33.0	33.0	33.0	27.0	33.0
3	31.703155508350314	33.0	33.0	33.0	27.0	33.0
4	31.832176247760664	33.0	33.0	33.0	27.0	33.0
5	31.93895480908468	33.0	33.0	33.0	33.0	33.0
6	34.799891812878826	37.0	37.0	37.0	27.0	37.0
7	34.95393994095293	37.0	37.0	37.0	27.0	37.0
8	35.121877654345184	37.0	37.0	37.0	33.0	37.0
9	35.20549019250318	37.0	37.0	37.0	33.0	37.0
10	35.22741783780745	37.0	37.0	37.0	33.0	37.0
11	35.26095280640735	37.0	37.0	37.0	33.0	37.0
12	35.20551906266192	37.0	37.0	37.0	33.0	37.0
13	35.24021947398571	37.0	37.0	37.0	33.0	37.0
14	35.19767093793068	37.0	37.0	37.0	33.0	37.0
15	35.24577925876627	37.0	37.0	37.0	33.0	37.0
16	35.25109288745652	37.0	37.0	37.0	33.0	37.0
17	35.205848790264376	37.0	37.0	37.0	33.0	37.0
18	35.21502190333359	37.0	37.0	37.0	33.0	37.0
19	35.21164561424302	37.0	37.0	37.0	33.0	37.0
20	35.2143396558981	37.0	37.0	37.0	33.0	37.0
21	35.20896828689037	37.0	37.0	37.0	33.0	37.0
22	35.22483927678733	37.0	37.0	37.0	33.0	37.0
23	35.192446958680726	37.0	37.0	37.0	33.0	37.0
24	35.207149466889724	37.0	37.0	37.0	33.0	37.0
25	35.194396454136715	37.0	37.0	37.0	33.0	37.0
26	35.16584386714257	37.0	37.0	37.0	33.0	37.0
27	35.10446590966072	37.0	37.0	37.0	33.0	37.0
28	35.076539349266625	37.0	37.0	37.0	33.0	37.0
29	35.11624797339083	37.0	37.0	37.0	33.0	37.0
30	35.07339402144597	37.0	37.0	37.0	33.0	37.0
31	35.06395044057382	37.0	37.0	37.0	27.0	37.0
32	35.093714054753015	37.0	37.0	37.0	33.0	37.0
33	35.07859216950126	37.0	37.0	37.0	33.0	37.0
34	34.99694128265557	37.0	37.0	37.0	27.0	37.0
35	34.999718895822795	37.0	37.0	37.0	27.0	37.0
36	34.968976735210504	37.0	37.0	37.0	27.0	37.0
37	34.9934327986276	37.0	37.0	37.0	27.0	37.0
38	34.855782920717985	37.0	37.0	37.0	27.0	37.0
39	34.91068788471386	37.0	37.0	37.0	27.0	37.0
40	34.94482760716527	37.0	37.0	37.0	27.0	37.0
41	34.96052537610979	37.0	37.0	37.0	27.0	37.0
42	34.867116737246604	37.0	37.0	37.0	27.0	37.0
43	34.64886289561614	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	21.0
16	28.0
17	32.0
18	25.0
19	24.0
20	54.0
21	159.0
22	400.0
23	892.0
24	1912.0
25	3311.0
26	5562.0
27	8460.0
28	11953.0
29	15752.0
30	20877.0
31	26325.0
32	33440.0
33	43292.0
34	60730.0
35	110330.0
36	314540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39099463774789	20.316082653744992	13.482212183510885	24.810710524996242
2	15.897580832645769	23.281351852780425	37.96805744857693	22.853009865996878
3	17.742839820761898	26.81460343798006	30.843813960697076	24.59874278056096
4	12.54529955828657	18.010268659619307	39.97377374000751	29.47065804208661
5	14.147289471964797	36.760828968621176	35.14546761300008	13.946413946413946
6	28.76273136013396	40.68489133424198	17.284108193199103	13.268269112424957
7	26.204835295744388	32.806528910425016	23.302472653122003	17.686163140708594
8	24.62199085575709	35.4245964635575	20.794719496018196	19.15869318466721
9	26.359974411922465	15.04241634111764	20.3604515292827	38.2371577176772
10	15.319873761432204	27.795581042334288	33.79130521987665	23.093239976356863
11	34.0813743411146	23.672618477813284	24.336024336024337	17.909982845047782
12	22.96666712251128	25.85687390882196	29.99168843324688	21.184770535419887
13	28.313420521212727	22.04327788743373	26.29433278783928	23.348968803514257
14	22.231845608468987	21.22959525556928	27.896018805109712	28.642540330852018
15	24.191521594119	28.24185291717759	24.655419460614265	22.911206028089147
16	22.72917207982143	28.47387782452718	25.38826564800591	23.408684447645488
17	22.27712617323007	27.36830269297802	26.567231762036958	23.787339371754957
18	23.440289674055908	26.698211113795526	27.77446024199271	22.087038970155852
19	24.885924885924886	26.079174131122183	27.546385987944426	21.4885149950085
20	24.547536235847925	25.976609093492208	27.361920868414373	22.11393380224549
21	23.274514183605095	26.95667500862306	27.404770261913118	22.364040545858728
22	22.921386557750196	26.603091538156477	27.628134121640613	22.847387782452717
23	22.98170999469701	26.539729137131733	28.17134895056973	22.307211917601528
24	23.09612699223089	26.576044757862938	27.059240046253034	23.268588203653138
25	23.400175348227297	26.584098012669443	27.84344472656161	22.172281912541653
26	23.06406592120878	26.971110087993207	27.374988413949453	22.589835576848564
27	22.96058919435543	27.127920634414142	27.22896619000515	22.68252398122528
28	22.672495399768128	26.84013073623463	27.826882372336918	22.660491491660324
29	23.542702763481984	26.773577422928074	27.141899869172597	22.541819944417345
30	23.534041715859896	26.64746041369418	27.61141981921203	22.207078051233893
31	23.452901374979295	26.376232869739365	27.17593626684536	22.994929488435982
32	22.32498985745739	26.47408751304855	27.95163184773574	23.249290781758315
33	22.390783429744467	26.64746041369418	28.19231780270741	22.76943835385394
34	22.960437246151532	26.451599178871906	27.61339514586268	22.974568429113884
35	22.84723583424882	27.16849080485444	27.43941445240147	22.54485890849527
36	23.217989451755685	26.869304791382714	27.134758303589475	22.77794745327213
37	23.268284307245345	26.06033255383905	27.39139881997025	23.279984318945356
38	23.154019257915362	26.47044075615504	27.27515844398961	23.10038154193998
39	22.59272259272259	26.623148701070782	27.791934285440778	22.99219442076585
40	22.78964746497214	26.777528076229373	28.049486491044934	22.38333796775355
41	22.847387782452717	25.993323395920797	27.855600582873308	23.303688238753175
42	22.320279463136604	27.021708839890657	27.740271896116052	22.917739800856683
43	22.914396940370967	26.091481935637777	27.734801760775785	23.259319363215468
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	28.0
2	45.0
3	189.0
4	333.0
5	333.0
6	428.0
7	523.0
8	494.5
9	466.0
10	628.5
11	791.0
12	791.0
13	1228.5
14	1666.0
15	2918.5
16	4171.0
17	4719.0
18	5267.0
19	5267.0
20	4805.0
21	4343.0
22	5004.0
23	5665.0
24	7433.5
25	9202.0
26	9202.0
27	11063.5
28	12925.0
29	15394.0
30	17863.0
31	21756.5
32	25650.0
33	25650.0
34	29619.5
35	33589.0
36	37008.0
37	40427.0
38	42630.0
39	44833.0
40	44833.0
41	47387.0
42	49941.0
43	51505.0
44	53069.0
45	52717.0
46	52365.0
47	52365.0
48	51343.0
49	50321.0
50	50155.0
51	49989.0
52	47340.5
53	44692.0
54	44692.0
55	42039.0
56	39386.0
57	34675.0
58	29964.0
59	27817.5
60	25671.0
61	25671.0
62	22193.0
63	18715.0
64	15464.5
65	12214.0
66	10573.5
67	8933.0
68	8933.0
69	7405.5
70	5878.0
71	4838.5
72	3799.0
73	3102.0
74	2405.0
75	2405.0
76	1857.5
77	1310.0
78	1029.0
79	748.0
80	583.5
81	419.0
82	419.0
83	332.0
84	245.0
85	205.5
86	166.0
87	122.5
88	79.0
89	79.0
90	55.5
91	32.0
92	20.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	658119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.45383756299769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.33420267581657	51.92353499581917
2	7.315579317463274	8.69878528839374
3	1.8820109437104018	3.3567831881742665
4	0.858867416270072	2.042518554202895
5	0.47893789068558434	1.4237347777792737
6	0.3281261221334579	1.1705014299299366
7	0.2321882557383821	0.9663137988493874
8	0.17895658741815626	0.8511724703349967
9	0.1422546193081668	0.7611824726040441
>10	1.0270188263710152	12.157459559375699
>50	0.13316585246262524	5.489476310192416
>100	0.08612072472023033	9.483235803121556
>500	0.0017995375314657815	0.653246781783188
>1k	7.71230370628192E-4	1.0220545694394427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3250	0.4938316626628315	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2338	0.35525490070944615	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1099	0.16699107608198516	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	696	0.10575594991179406	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	682	0.10362867505724649	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.038964077925117E-4	0.0	0.0
2	0.0	0.0	3.038964077925117E-4	0.0	0.0
3	0.0	0.0	0.0010636374272737909	0.0	0.0
4	0.0	0.0	0.0010636374272737909	1.5194820389625584E-4	0.0
5	0.0	0.0	0.0016714302428588142	3.038964077925117E-4	0.0
6	0.0	0.0	0.003950653301302652	3.038964077925117E-4	0.0
7	0.0	0.0	0.011396115292219188	3.038964077925117E-4	0.0
8	0.0	0.0	0.013067545535078003	3.038964077925117E-4	0.0
9	0.0	0.0	0.01641040602079563	9.11689223377535E-4	0.0
10	7.597410194812792E-4	0.0	0.01868962907923947	9.11689223377535E-4	0.0
11	7.597410194812792E-4	0.0	0.025831194662363492	9.11689223377535E-4	0.0
12	9.11689223377535E-4	0.0	0.028414314128599842	0.0010636374272737909	0.0
13	9.11689223377535E-4	0.0	0.02962989975976989	0.0010636374272737909	0.0
14	9.11689223377535E-4	0.0	0.03145327820652496	0.0010636374272737909	0.0
15	9.11689223377535E-4	0.0	0.03494808689613885	0.0012155856311700467	0.0
16	9.11689223377535E-4	0.0	0.0378351027701677	0.0012155856311700467	0.0
17	9.11689223377535E-4	0.0	0.03844289558575273	0.0013675338350663026	0.0
18	9.11689223377535E-4	0.0	0.03996237762471529	0.0013675338350663026	0.0
19	9.11689223377535E-4	0.0	0.04102601505198908	0.0013675338350663026	0.0
20	9.11689223377535E-4	0.0	0.041481859663677845	0.0013675338350663026	0.0
21	9.11689223377535E-4	0.0	0.04391303092601794	0.0015194820389625584	0.0
22	0.0010636374272737909	0.0	0.04436887553770671	0.0016714302428588142	0.0
23	0.0010636374272737909	0.0	0.04436887553770671	0.0021272748545475817	0.0
24	0.0010636374272737909	0.0	0.04436887553770671	0.002279223058443838	0.0
25	0.0010636374272737909	0.0	0.04436887553770671	0.002279223058443838	0.0
26	0.0010636374272737909	0.0	0.04452082374160296	0.002887015874028861	0.0
27	0.0010636374272737909	0.0	0.04467277194549922	0.0042545497090951635	0.0
28	0.0012155856311700467	0.0	0.04467277194549922	0.013979234758455538	0.0
29	0.0012155856311700467	0.0	0.04467277194549922	0.036315620731205145	0.0
30	0.0012155856311700467	0.0	0.04467277194549922	0.06594552049097503	0.0
31	0.0012155856311700467	0.0	0.04467277194549922	0.15194820389625585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATCG	35	8.86535E-4	26.428572	5
GGTATCA	465	0.0	25.064516	1
GTATCAA	1330	0.0	24.7594	1
TCTAAGC	70	1.9203253E-7	23.785715	3
ACGATCG	40	0.0019303153	23.125	16
GCACCGC	145	0.0	21.689655	10
TTAACGG	145	0.0	21.689655	35
AACGATT	70	5.0964554E-6	21.142859	22
CGCCTAT	45	0.0038240973	20.555555	34
AACGGCC	145	0.0	20.413794	37
TAACGGC	150	0.0	19.733332	36
GGACCGT	75	9.255909E-6	19.733332	6
ACTAGTG	60	9.231096E-4	18.5	8
CGCCGTT	60	9.231096E-4	18.5	25
TCTAATA	210	0.0	17.61905	2
GTTTAAC	190	0.0	17.526314	33
CTCTAAT	180	0.0	17.472221	1
GTGACAC	205	0.0	17.146343	24
TAATACT	270	0.0	17.12963	4
CCGATGT	65	0.0015789637	17.076923	14
>>END_MODULE