FastQCFastQC Report
Fri 10 Feb 2017
ERR1633067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410689
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11170.27198196201992264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.2227963251998471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4580.11151990922571582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA352.3845982E-531.7142872
ACGGTAC451.3211898E-424.6666663
GTATCAA7350.024.4149671
CCTTAGG551.8988005E-523.5454541
GCCGGCA551.8988005E-523.54545415
TGCTCGC502.6980054E-422.210
TCACGGT450.003821684320.5555551
GAATCGT450.003821684320.5555556
AGGACCG450.003821684320.5555555
GTCTTAT450.003821684320.5555551
CTACGGT555.135361E-420.18181827
CCGATGA500.007027371318.59
TACGGTG609.223042E-418.528
GCCGATG500.007027371318.58
TAGGACC1103.833702E-818.54
GTGACCG500.007027371318.517
AATCGTG500.007027371318.57
GGTATCA2550.018.1372551
CTGCTCG1800.017.4722219
CCGGCAG752.0631925E-417.26666616
Produced by FastQC (version 0.11.5)