FastQCFastQC Report
Fri 10 Feb 2017
ERR1633066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455324
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12070.2650859607663993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.17130658607936328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACC556.247792E-726.9090915
AGATCGT358.8611373E-426.4285719
GTATCAA8500.024.376471
GGTATCA3050.022.4426211
CCGTACA450.003822313420.55555517
CCGTTAA450.003822313420.55555516
TTAACGG555.13654E-420.18181835
CTAGACA1254.0927262E-1019.2400024
ACGGGTA801.6131784E-518.516
TCTAGAC1205.149559E-918.53
TACCCTC1002.865454E-718.4999985
CTATAAC500.00702851918.4999983
TCGCTCG500.00702851918.49999827
GTTCTAG1351.1459633E-917.8148141
TATTAAG752.0638134E-417.2666662
CGATACA650.00157795417.07692336
TCAACCC1201.0370968E-716.95833416
TATACTG1107.782164E-716.8181825
ATCACGT1005.8621245E-616.6524
ATCAACG12600.016.5912693
Produced by FastQC (version 0.11.5)