##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455324
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.736963129551704	33.0	33.0	33.0	27.0	33.0
2	31.247039470794423	33.0	33.0	33.0	27.0	33.0
3	31.687598281663167	33.0	33.0	33.0	27.0	33.0
4	31.821650077746835	33.0	33.0	33.0	27.0	33.0
5	31.92701680561534	33.0	33.0	33.0	33.0	33.0
6	34.83864017710466	37.0	37.0	37.0	27.0	37.0
7	35.0094943380977	37.0	37.0	37.0	33.0	37.0
8	35.16681527879049	37.0	37.0	37.0	33.0	37.0
9	35.24357600302202	37.0	37.0	37.0	33.0	37.0
10	35.284865282743716	37.0	37.0	37.0	33.0	37.0
11	35.30959492581107	37.0	37.0	37.0	33.0	37.0
12	35.26959044548497	37.0	37.0	37.0	33.0	37.0
13	35.281858632534195	37.0	37.0	37.0	33.0	37.0
14	35.25861145030791	37.0	37.0	37.0	33.0	37.0
15	35.29952078080663	37.0	37.0	37.0	33.0	37.0
16	35.30455016647486	37.0	37.0	37.0	33.0	37.0
17	35.25163180504432	37.0	37.0	37.0	33.0	37.0
18	35.2495717335348	37.0	37.0	37.0	33.0	37.0
19	35.254153086593284	37.0	37.0	37.0	33.0	37.0
20	35.25924835941	37.0	37.0	37.0	33.0	37.0
21	35.27100482293927	37.0	37.0	37.0	33.0	37.0
22	35.261574175751775	37.0	37.0	37.0	33.0	37.0
23	35.254150890355	37.0	37.0	37.0	33.0	37.0
24	35.246639755426905	37.0	37.0	37.0	33.0	37.0
25	35.25370725022182	37.0	37.0	37.0	33.0	37.0
26	35.21460762006835	37.0	37.0	37.0	33.0	37.0
27	35.153831118060985	37.0	37.0	37.0	33.0	37.0
28	35.11710342525323	37.0	37.0	37.0	33.0	37.0
29	35.16899175092901	37.0	37.0	37.0	33.0	37.0
30	35.13502692588135	37.0	37.0	37.0	33.0	37.0
31	35.114210979434425	37.0	37.0	37.0	33.0	37.0
32	35.141284008749814	37.0	37.0	37.0	33.0	37.0
33	35.13466893904121	37.0	37.0	37.0	33.0	37.0
34	35.07135358557862	37.0	37.0	37.0	33.0	37.0
35	35.077347119853115	37.0	37.0	37.0	33.0	37.0
36	35.048398503043984	37.0	37.0	37.0	27.0	37.0
37	35.0805668051761	37.0	37.0	37.0	33.0	37.0
38	34.9322109091548	37.0	37.0	37.0	27.0	37.0
39	35.00517214115663	37.0	37.0	37.0	27.0	37.0
40	35.03715156679639	37.0	37.0	37.0	27.0	37.0
41	35.06973715420228	37.0	37.0	37.0	33.0	37.0
42	34.980488619093215	37.0	37.0	37.0	27.0	37.0
43	34.77576846377524	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	17.0
17	16.0
18	17.0
19	16.0
20	30.0
21	102.0
22	254.0
23	581.0
24	1241.0
25	2302.0
26	3671.0
27	5486.0
28	7875.0
29	10664.0
30	13759.0
31	17479.0
32	22459.0
33	29269.0
34	41897.0
35	76814.0
36	221369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.079099718003	20.28621377304952	13.35664274231097	25.278043766636504
2	16.201210566541626	22.701417012940237	37.834377278597216	23.262995141920918
3	18.04627034814769	26.217814127961624	30.038829492844656	25.697086031046023
4	12.8400874981332	17.67378833533923	38.92327221934271	30.56285194718486
5	14.18133021760329	36.61722202212051	35.21887710729063	13.982570652985565
6	30.162916955838043	39.50220063075964	17.127803498168337	13.20707891523399
7	27.035473640748126	31.834034665425058	23.216654514148168	17.913837179678644
8	24.70548444624048	35.72379228856814	20.502543243931793	19.068180021259586
9	26.013344343807926	15.286038073986873	20.200560480009838	38.50005710219536
10	14.753450290342702	28.161485008477477	33.83370962215916	23.25135507902065
11	34.29250379949223	23.46746492607462	24.148957665310856	18.091073609122297
12	23.318999218139172	25.31516019362037	30.287224042659734	21.07861654558073
13	28.15797102722457	22.079222707346858	26.025423654364804	23.73738261106377
14	22.25184703639606	20.804086760197134	27.199971888149975	29.74409431525683
15	23.928894589347365	28.59370470258541	24.04946807108784	23.42793263697938
16	23.50172624329049	28.1206349764124	24.67385861496429	23.70378016533282
17	22.755224850875422	27.453198162187803	26.101852746615595	23.689724240321176
18	23.58254781210742	26.949600723880142	27.179986119774053	22.28786534423839
19	24.953439748398942	26.20705256037459	26.670898085758715	22.168609605467754
20	24.483884003478842	26.285677890908453	26.87426975077088	22.356168354841827
21	23.608243800019327	26.6034735704685	26.896232133601565	22.892050495910603
22	23.151426237141028	26.733930124482786	26.799817272974845	23.314826365401338
23	23.130342349623565	26.460059210584113	27.20809796979733	23.201500469994993
24	23.23620103486748	26.939937275434634	26.36320510230078	23.460656587397104
25	23.809858474405036	26.697252945155537	26.891839657035433	22.601048923403994
26	23.844339415449216	26.581511187637812	26.61994535759152	22.95420403932145
27	23.385325614287847	26.732173133856328	26.574702848960303	23.30779840289552
28	23.02733877414764	26.441610809006335	27.030641916525376	23.500408500320653
29	23.790531577514034	26.56591789582803	26.36320510230078	23.28034542435716
30	23.52368862612118	26.45456861487644	26.925442102766382	23.096300656236
31	23.647776089114565	26.342340838611626	26.792350062812414	23.217533009461395
32	22.71173933287066	26.449956514481993	27.084889002117173	23.75341515053017
33	23.094543665609542	26.47147964965607	27.331746185134104	23.102230499600285
34	23.353260535355044	26.088236069260574	27.029983045040456	23.528520350343932
35	23.24806072159605	26.87558749374072	26.845279405434376	23.031072379228856
36	24.034314026934666	26.342340838611626	26.5907353884267	23.032609746027006
37	23.54543138512356	26.106904094666657	26.765555955758973	23.58210856445081
38	23.503702857745253	26.4341435988439	26.791471567499187	23.270681975911657
39	23.29154623960081	26.458521843785967	27.107071008776167	23.142860907837058
40	23.318999218139172	26.77719601865924	26.95289508130474	22.950909681896846
41	23.405091758835468	25.902214686684648	27.157364865458444	23.535328689021444
42	23.11606680078362	26.58700178334549	27.013730881745744	23.28320053412515
43	23.3870826049143	26.065614814944958	27.07764141578305	23.46966116435769
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	70.5
4	126.0
5	126.0
6	161.5
7	197.0
8	186.0
9	175.0
10	225.5
11	276.0
12	276.0
13	433.5
14	591.0
15	1035.0
16	1479.0
17	1833.0
18	2187.0
19	2187.0
20	2176.0
21	2165.0
22	2709.5
23	3254.0
24	4294.0
25	5334.0
26	5334.0
27	7192.0
28	9050.0
29	10851.0
30	12652.0
31	14999.0
32	17346.0
33	17346.0
34	20161.0
35	22976.0
36	24941.5
37	26907.0
38	29130.0
39	31353.0
40	31353.0
41	32627.0
42	33901.0
43	35015.5
44	36130.0
45	36261.5
46	36393.0
47	36393.0
48	35578.0
49	34763.0
50	34616.0
51	34469.0
52	33324.5
53	32180.0
54	32180.0
55	30613.0
56	29046.0
57	25487.5
58	21929.0
59	20369.0
60	18809.0
61	18809.0
62	16585.5
63	14362.0
64	12036.0
65	9710.0
66	8045.5
67	6381.0
68	6381.0
69	5474.0
70	4567.0
71	3718.0
72	2869.0
73	2222.5
74	1576.0
75	1576.0
76	1226.5
77	877.0
78	659.0
79	441.0
80	385.0
81	329.0
82	329.0
83	251.5
84	174.0
85	181.0
86	188.0
87	135.0
88	82.0
89	82.0
90	61.5
91	41.0
92	27.5
93	14.0
94	9.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.35000735695485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.90563770951486	56.792840636776475
2	7.063350659599403	9.231800351391458
3	2.0659256270430517	4.050247647785549
4	1.043242844233164	2.7270371018291106
5	0.6288375495199559	2.0547269243729285
6	0.4111194580579998	1.6119995765206545
7	0.2973924016940343	1.360421694702533
8	0.22638933656668955	1.183563584813543
9	0.16505104006680085	0.9707478014374635
>10	1.085023691949725	13.113560593338402
>50	0.07788973926152128	3.4059375009050292
>100	0.029462640414354962	3.057376638232021
>500	3.3865103924545935E-4	0.1726206136678231
>1k	3.3865103924545935E-4	0.26711933422700324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.2650859607663993	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	780	0.17130658607936328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	4.3924765661375195E-4	0.0	0.0
6	0.0	0.0	0.00109811914153438	0.0	0.0
7	0.0	0.0	0.00329435742460314	0.0	0.0
8	0.0	0.0	0.0039532289095237675	0.0	0.0
9	0.0	0.0	0.00439247656613752	0.0	0.0
10	2.1962382830687598E-4	0.0	0.005051348051058148	0.0	0.0
11	2.1962382830687598E-4	0.0	0.007027962505820031	2.1962382830687598E-4	0.0
12	2.1962382830687598E-4	0.0	0.007906457819047535	4.3924765661375195E-4	0.0
13	2.1962382830687598E-4	0.0	0.008345705475661287	4.3924765661375195E-4	0.0
14	2.1962382830687598E-4	0.0	0.008565329303968163	4.3924765661375195E-4	0.0
15	2.1962382830687598E-4	0.0	0.00988307227380942	4.3924765661375195E-4	0.0
16	2.1962382830687598E-4	0.0	0.011640062900264427	4.3924765661375195E-4	0.0
17	2.1962382830687598E-4	0.0	0.012079310556878178	4.3924765661375195E-4	0.0
18	2.1962382830687598E-4	0.0	0.012079310556878178	4.3924765661375195E-4	0.0
19	2.1962382830687598E-4	0.0	0.012298934385185055	4.3924765661375195E-4	0.0
20	2.1962382830687598E-4	0.0	0.012957805870105683	4.3924765661375195E-4	0.0
21	2.1962382830687598E-4	0.0	0.013616677355026311	6.58871484920628E-4	0.0
22	2.1962382830687598E-4	0.0	0.014055925011640063	8.784953132275039E-4	0.0
23	2.1962382830687598E-4	0.0	0.014055925011640063	8.784953132275039E-4	0.0
24	2.1962382830687598E-4	0.0	0.014055925011640063	0.0015373667981481319	0.0
25	2.1962382830687598E-4	0.0	0.014055925011640063	0.0015373667981481319	0.0
26	2.1962382830687598E-4	0.0	0.014055925011640063	0.0017569906264550078	0.0
27	2.1962382830687598E-4	0.0	0.014055925011640063	0.0030747335962962637	0.0
28	2.1962382830687598E-4	0.0	0.014055925011640063	0.008345705475661287	0.0
29	2.1962382830687598E-4	0.0	0.014055925011640063	0.019107273062698212	0.0
30	2.1962382830687598E-4	0.0	0.014055925011640063	0.03557906018571391	0.0
31	2.1962382830687598E-4	0.0	0.014055925011640063	0.08938689812089852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACC	55	6.247792E-7	26.90909	15
AGATCGT	35	8.8611373E-4	26.42857	19
GTATCAA	850	0.0	24.37647	1
GGTATCA	305	0.0	22.442621	1
CCGTACA	45	0.0038223134	20.555555	17
CCGTTAA	45	0.0038223134	20.555555	16
TTAACGG	55	5.13654E-4	20.181818	35
CTAGACA	125	4.0927262E-10	19.240002	4
ACGGGTA	80	1.6131784E-5	18.5	16
TCTAGAC	120	5.149559E-9	18.5	3
TACCCTC	100	2.865454E-7	18.499998	5
CTATAAC	50	0.007028519	18.499998	3
TCGCTCG	50	0.007028519	18.499998	27
GTTCTAG	135	1.1459633E-9	17.814814	1
TATTAAG	75	2.0638134E-4	17.266666	2
CGATACA	65	0.001577954	17.076923	36
TCAACCC	120	1.0370968E-7	16.958334	16
TATACTG	110	7.782164E-7	16.818182	5
ATCACGT	100	5.8621245E-6	16.65	24
ATCAACG	1260	0.0	16.591269	3
>>END_MODULE