FastQCFastQC Report
Fri 10 Feb 2017
ERR1633065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423851
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94362.2262540373857793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74701.7624117909359658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36330.8571408348688572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.4680890218496594No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18020.4251494039178862No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16600.3916470646524368No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16440.38787215318590734No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14020.33077661725464846No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12500.29491495832261805No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12380.29208377472272096No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.24914415679094778No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9810.2314492592915907No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9720.2293258715916678No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9620.2269665519250869No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9520.22460723225850593No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9480.22366350439187357No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8810.2078560626257812No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8770.20691233475914886No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8400.19818285199279936No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8350.1970031921595089No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8050.18992523315976606No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7890.18615032169323653No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7860.18544252579326226No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7810.18426286595997177No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7550.17812863482686134No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7340.17317406352704134No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7200.16987101599382803No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7160.16892728812719562No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.1670398323939309No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6940.16373678486071758No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6880.162321193060769No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6810.16066966929416232No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6760.15949000946087186No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6760.15949000946087186No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6750.15925407749421377No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6710.1583103496275814No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.1545354381610519No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6430.15170425456115474No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6410.15123239062783855No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6370.1502886627612062No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6090.14368256769477955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.14273883982814714No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5880.13872799639495953No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5790.13660460869503668No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5790.13660460869503668No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5760.1358968127950624No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5740.13542494886174622No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5690.13424528902845576No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5500.12976258166195195No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5230.1233924185621834No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5170.12197682676223486No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5000.11796598332904724No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4950.11678632349575677No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4950.11678632349575677No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4940.11655039152909867No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4900.1156066636624663No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4870.114898867762492No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4750.11206768416259488No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4720.1113598882626206No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4720.1113598882626206No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4690.1106520923626463No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4600.10852870466272345No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4570.10782090876274918No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4480.10569752106282633No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4450.10498972516285204No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4390.10357413336290347No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4370.10310226942958729No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4310.10168667762963872No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4290.10121481369632253No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4280.10097888172966443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGG303.5956793E-430.8333349
CATGTTT303.5956793E-430.83333430
CACATGT358.860122E-426.4285728
ACATGTT358.860122E-426.4285729
GTGACAC358.860122E-426.4285724
GGTATCA14150.025.1024721
ACTAGTG601.3340814E-624.6666688
CATACTA1051.8189894E-1224.6666665
CCTAGCT701.9169784E-723.7857134
GTTTAAC400.001929187223.12533
CGACAAA400.001929187223.12517
CGTTACG400.001929187223.12516
TGCGTCT400.001929187223.12510
GTATCAA38250.022.9254911
TCCGCCA903.810783E-922.6111112
CGCCACT903.810783E-922.6111114
TCATACT1155.456968E-1222.5217384
GAATCAA502.6982045E-422.21
TCGGGTA603.7191465E-521.58333425
AAGTTCC603.7191465E-521.58333431
Produced by FastQC (version 0.11.5)