FastQCFastQC Report
Fri 10 Feb 2017
ERR1633064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619401
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28470.4596376176338107No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC24500.3955434363199285No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC23760.38359641008006123No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG22130.3572806630922456No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG20930.337907107027596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.32822032899527126No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA20210.3262829733888063No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG19810.31982512136725644No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG19320.31191425264085787No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT18310.2956081762864445No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT17710.2859213982541197No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC17290.2791406536314924No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC17140.27671895912341116No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA16700.26961532189970633No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG16120.26025143646845905No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA15100.24378391381350692No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT14860.23990920260057702No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG14320.2311911023714847No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC13960.22537903555208985No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC13920.22473325034993485No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT13720.22150432433915992No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG13400.21633804272192003No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13080.21117176110468017No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG12940.20891151289713772No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG12790.20648981838905656No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT12580.20309944607774283No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC12540.20245366087558786No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA12360.19954762746589044No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC11690.18873072532979443No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG11610.18743915492548446No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC11440.1846945678163258No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT10980.17726803799154345No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT10810.17452345088238477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10220.16499811915059873No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA10180.16435233394844373No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10170.16419088764790502No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC9940.16047762273551383No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9870.1593474986317426No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9820.15854026712904887No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA9680.15628001892150642No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC9230.14901493539726285No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG9160.14788481129349162No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT9120.14723902609133663No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT9070.1464317945886429No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG8940.1443329926816392No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC8850.14287997597679047No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC8840.14271852967625173No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT8810.1422341907746355No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8690.14029683516817054No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA8230.13287030534338823No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG8180.13206307384069446No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA8160.13174018123961698No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8100.1307715034363845No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG8090.13061005713584575No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG8070.13028716453476827No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT8060.13012571823422953No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7940.12818836262776456No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT7920.12786547002668708No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7890.12738113112507082No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA7890.12738113112507082No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT7830.12641245332183837No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG7770.1254437755186059No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7720.12463654401591215No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG7600.12269918840944719No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT7470.1206003865024435No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT7310.11801724569382355No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7310.11801724569382355No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA7250.11704856789059107No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC7200.11624133638789734No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7170.1157569974862811No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA6910.11155939367227369No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT6780.10946059176527No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC6610.1067160046561113No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA6510.10510154165072384No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA6470.10445575644856886No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG6270.10122683043779393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG200.001841280637.08
CTAGTGC200.001841280637.09
GAATCAA401.5984897E-632.3751
TAGCGAG303.5975792E-430.83333228
CGAGGCT303.5975792E-430.83333231
TCTTCAG250.00549441129.616
TTACTTC250.00549441129.629
CGGTCAA250.00549441129.612
ACGGTCA250.00549441129.611
ACGTTTA358.8647567E-426.42857226
TAGAACA358.8647567E-426.4285724
CGTTAGA358.8647567E-426.4285721
AATTATC509.082252E-625.90000230
GGTATCA6000.024.9751
CGGGTAT451.3222497E-424.66666817
GAACTTA451.3222497E-424.66666825
AGGACGC451.3222497E-424.66666812
CGCCGCC1002.0008883E-1124.05000133
CCGGCAG1400.023.78571516
GTATCAA18950.023.3324551
Produced by FastQC (version 0.11.5)