FastQCFastQC Report
Fri 10 Feb 2017
ERR1633063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646200
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42350.6553698545341999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.4797276385020118No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17780.27514701330857316No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17370.26880222841225626No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16930.2619931909625503No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16610.2570411637264005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16160.25007737542556485No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15500.2398638192510059No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG13830.2140204271123491No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13830.2140204271123491No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13540.20953265242958838No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13440.20798514391829154No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13270.205354379449087No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13120.20303311668214175No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12900.19962859795728877No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12780.1977715877437326No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT12460.19281956050758278No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG12420.19220055710306405No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12050.18647477561126585No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11790.18245125348189414No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11660.1804394924172083No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11480.17765397709687403No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10930.16914268028474158No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10740.16620241411327763No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10730.16604766326214795No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10730.16604766326214795No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10370.16047663262147943No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9760.15103683070256887No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC9680.1497988238935314No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9640.14917982048901268No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC9620.14887031878675333No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9440.14608480346641906No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9430.14593005261528938No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG9240.14298978644382543No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9090.14066852367688024No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9050.14004952027236148No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8940.13834726090993502No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG8670.13416898792943363No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8590.13293098112039614No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8570.13262147941813682No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8550.13231197771587744No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8470.13107397090684No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8440.13060971835345095No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8370.1295264623955432No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8260.12782420303311667No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT8210.1270504487774683No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8070.12488393686165274No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7850.12147941813679976No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7810.12086041473228103No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT7770.12024141132776231No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7730.11962240792324358No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7670.1186939028164655No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7640.11822965026307644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.11683689260290932No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7480.11575363664500156No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7460.1154441349427422No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7450.1152893840916125No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7330.11343237387805633No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7330.11343237387805633No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7330.11343237387805633No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7250.11219436706901888No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7220.11173011451562985No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7010.10848034664190653No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6980.10801609408851748No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6920.1070875889817394No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6780.10492107706592385No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6740.10430207366140515No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6730.10414732281027546No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6730.10414732281027546No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6720.10399257195914577No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG6650.10290931600123801No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6610.10229031259671928No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6590.10198081089445991No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6540.10120705663881151No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6510.10074280408542247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGG251.2330277E-437.09
TCTGTCC250.005494585329.636
CGTTTAT250.005494585329.626
ATCTAAC405.936168E-527.7520
TCTAACT405.936168E-527.7521
CGGAGCC405.936168E-527.7532
GGTATCA9350.027.3048111
GGCACTA358.865175E-426.42857226
TACTCGC509.083049E-625.90000220
TTAGCTA951.0913936E-1125.315794
TTACTCG601.3358931E-624.66666819
CCCACAA451.3223362E-424.66666833
CTAACTT451.3223362E-424.66666822
ACTCGCA601.3358931E-624.66666821
ATGCACT451.3223362E-424.66666811
GTATCAA28050.024.3368971
ACCGTTT851.9717845E-923.94117710
GCCGCTC1650.023.54545427
CTCGCAT551.9009885E-523.54545422
TATACGA551.9009885E-523.54545436
Produced by FastQC (version 0.11.5)