FastQCFastQC Report
Fri 10 Feb 2017
ERR1633062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662779
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37430.5647433005571992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.42593383314800254No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19770.2982894750738934No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC19240.2902928427122766No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG18830.28410676862121464No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17180.25921159240108693No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG16420.24774472335424028No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15260.2302426600722111No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15250.23009178021633153No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG15190.22918650108105418No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC14730.22224602771059432No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14710.22194426799883518No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT14650.22103898886355783No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT14430.21771963203420747No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA14360.21666347304305053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13880.2094212399608316No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG13780.20791244140203596No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT13000.19614381264343017No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12750.19237181624644112No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC12170.18362078460542652No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12170.18362078460542652No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG11440.17260655512621853No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11280.17019247743214555No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG11120.16777839973807257No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10880.1641572831969631No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC10590.1597817673764558No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG10420.15721680982650324No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10380.15661329040298502No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10330.1558588911235872No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10210.15404833285303246No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT10190.15374657314127332No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA10170.15344481342951422No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT10080.15208689472659817No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9950.15012545660016385No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG9860.14876753789724778No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC9760.14725873933845218No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9710.14650434005905436No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC9270.13986562640035366No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9260.1397147465444741No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC9240.13941298683271497No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA8970.1353392307239668No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8950.1350374710122077No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8810.13292515302989383No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT8810.13292515302989383No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG8770.13232163360637558No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG8640.1303601954799413No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT8550.12900227677702522No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC8420.12704083865059093No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT7990.12055300484776978No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7990.12055300484776978No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT7890.11904420628897415No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7840.11828980700957635No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7830.11813892715369677No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.11678100845078072No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC7730.11663012859490117No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7670.11572484945962379No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7660.11557396960374423No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7620.11497045018022597No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA7560.11406517104494862No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG7440.11225461277439387No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC7420.11195285306263476No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT7370.11119845378323695No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC7330.11059493435971869No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA7140.10772821709800703No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG7130.10757733724212748No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7080.10682293796272965No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT7050.10637029839509098No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT6970.10516325954805447No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA6950.10486149983629535No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC6940.1047106199804158No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6890.10395622070101798No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6800.10259830199810192No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC6710.10124038329518588No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA6690.10093862358342676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAGT200.001841373737.016
TGTATAT251.2330618E-437.03
CGGATCG200.001841373737.037
TATTAAG200.001841373737.015
ACTCGCA750.032.06666621
GGTATCA7500.030.0933321
GTAATTG250.005494685429.613
GCACAGG250.005494685429.620
AAGTTGT250.005494685429.619
GTGTATA250.005494685429.62
CTTTATG250.005494685429.61
TACTCGC851.8189894E-1228.29411720
TTACTCG1000.027.75000219
GGCAGCT1950.027.51282118
CTCGCAT905.456968E-1226.72222122
GGTACGA358.865417E-426.42857231
CGCCGGC2100.026.4285714
GCAGCTT2050.026.1707319
CGGCAGC2100.025.54761917
CCGCTCT2050.025.26829128
Produced by FastQC (version 0.11.5)