FastQCFastQC Report
Fri 10 Feb 2017
ERR1633061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599694
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41440.6910190864007311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.5124280049491907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15080.25146157873848995No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12150.20260332769712555No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11990.19993530033650497No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11460.19109745970444925No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10240.17075375107971733No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9510.1585808762468859No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9160.15274456639552839No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8330.13890417446230913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.12823206501982679No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7240.1207282380680814No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6860.1143916730866075No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6670.11122339059587057No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6610.11022288033563785No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6610.11022288033563785No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6240.10405306706420274No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6130.1022187982537761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCA250.005494273729.59999810
GGTATCA6800.027.4779431
GTATCAA23100.025.4675331
CGTTATT400.001930116223.12510
AGAGCGA400.001930116223.1256
CGCCGTC2550.022.49019626
GAGTCGC2450.021.8979622
CGAGTCG2650.021.6415121
CACATGT1059.804353E-1021.14285928
TGTTACG450.003823705720.55555716
GGACCGT1001.2867531E-820.3499986
TAACTAG1001.2867531E-820.34999826
GCCGTCC2550.020.31372527
GCCGGCA2850.020.12280815
TTCGCCG2950.020.06779724
AGTCGCC2700.019.8703723
TGTCCGG3300.019.62121214
GTGACAC1052.2540917E-819.38095324
CGCTCTC3150.019.3809529
GGCGAGT3150.019.3809519
Produced by FastQC (version 0.11.5)