##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599694
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719270161115503	33.0	33.0	33.0	27.0	33.0
2	31.22175642911218	33.0	33.0	33.0	27.0	33.0
3	31.66973489813138	33.0	33.0	33.0	27.0	33.0
4	31.791156823313223	33.0	33.0	33.0	27.0	33.0
5	31.90632555936861	33.0	33.0	33.0	33.0	33.0
6	34.74101625162166	37.0	37.0	37.0	27.0	37.0
7	34.91560028948097	37.0	37.0	37.0	27.0	37.0
8	35.09873368751396	37.0	37.0	37.0	33.0	37.0
9	35.196335130916765	37.0	37.0	37.0	33.0	37.0
10	35.20171954363392	37.0	37.0	37.0	33.0	37.0
11	35.230682648150555	37.0	37.0	37.0	33.0	37.0
12	35.18975510843864	37.0	37.0	37.0	33.0	37.0
13	35.20974196840388	37.0	37.0	37.0	33.0	37.0
14	35.17161919245482	37.0	37.0	37.0	33.0	37.0
15	35.22065753534302	37.0	37.0	37.0	33.0	37.0
16	35.22487301857281	37.0	37.0	37.0	33.0	37.0
17	35.178162529556744	37.0	37.0	37.0	33.0	37.0
18	35.18566135395719	37.0	37.0	37.0	33.0	37.0
19	35.19293839858328	37.0	37.0	37.0	33.0	37.0
20	35.18334350518764	37.0	37.0	37.0	33.0	37.0
21	35.17402541963068	37.0	37.0	37.0	33.0	37.0
22	35.176561713140366	37.0	37.0	37.0	33.0	37.0
23	35.16910791170164	37.0	37.0	37.0	33.0	37.0
24	35.161380637458436	37.0	37.0	37.0	33.0	37.0
25	35.166356508486	37.0	37.0	37.0	33.0	37.0
26	35.126372783452894	37.0	37.0	37.0	33.0	37.0
27	35.07516166578288	37.0	37.0	37.0	33.0	37.0
28	35.042571711572904	37.0	37.0	37.0	27.0	37.0
29	35.08016421708405	37.0	37.0	37.0	33.0	37.0
30	35.03853298515576	37.0	37.0	37.0	27.0	37.0
31	35.01427227886222	37.0	37.0	37.0	27.0	37.0
32	35.054159287903495	37.0	37.0	37.0	27.0	37.0
33	35.034792744299594	37.0	37.0	37.0	27.0	37.0
34	34.96533231948294	37.0	37.0	37.0	27.0	37.0
35	34.942712450016174	37.0	37.0	37.0	27.0	37.0
36	34.935168602654024	37.0	37.0	37.0	27.0	37.0
37	34.957573362414834	37.0	37.0	37.0	27.0	37.0
38	34.78940759787491	37.0	37.0	37.0	27.0	37.0
39	34.84935483763386	37.0	37.0	37.0	27.0	37.0
40	34.869616837920674	37.0	37.0	37.0	27.0	37.0
41	34.88364232425204	37.0	37.0	37.0	27.0	37.0
42	34.783906458960736	37.0	37.0	37.0	27.0	37.0
43	34.547087347880755	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	35.0
17	36.0
18	21.0
19	26.0
20	40.0
21	129.0
22	355.0
23	834.0
24	1812.0
25	3183.0
26	5116.0
27	7716.0
28	11077.0
29	14815.0
30	19427.0
31	24689.0
32	31006.0
33	40608.0
34	56705.0
35	101333.0
36	280712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.929060487515294	18.76857197170557	13.639956377752654	24.662411163026476
2	17.006506651725715	22.84715204754425	37.01737886322024	23.1289624375098
3	18.707207342411298	26.62591254873319	29.466694680953953	25.200185427901562
4	12.866228443172684	18.00051359526692	38.387577664608955	30.745680296951445
5	15.148892601893632	35.69387054064239	33.525431303298014	15.63180555416596
6	29.234409548869923	39.36057389268527	16.89044746153872	14.514569096906088
7	27.526705286362713	31.768702038039397	21.748425030098684	18.956167645499207
8	25.548029495042474	33.63632119047381	19.72072423602704	21.09492507845668
9	27.433491080451027	14.427191200845765	18.94999783222777	39.18931988647544
10	16.6513255093431	26.609904384569464	31.77353783763052	24.965232268456912
11	35.608326913392496	23.339403095578746	22.46512387984539	18.58714611118337
12	24.507332072690406	25.160665272622374	28.223727434324843	22.108275220362387
13	30.179891744789845	21.477953756415772	24.569030205404758	23.77312429338963
14	23.51749392190017	20.455265518747893	27.02094734981507	29.006293209536864
15	25.879031639469463	26.682107875016257	24.074444633429714	23.364415852084562
16	23.17915470223147	26.861866218438067	24.4724809652923	25.48649811403816
17	23.746944274913538	25.48232932128719	24.788141952395723	25.98258445140355
18	25.309407797976966	24.41311735651849	26.55520982367674	23.7222650218278
19	27.322100938145123	24.337412080160885	26.298412190217014	22.04207479147699
20	26.706787128102	23.913862736662363	26.16934636664699	23.210003768588646
21	24.7966462896077	25.359099807568526	26.3766187422252	23.467635160598572
22	24.272045409825676	25.048774875186346	26.394461175199353	24.284718539788624
23	24.115632305809296	25.002084396375484	27.32643648260613	23.55584681520909
24	24.095121845474527	25.369771917011008	26.01243300750049	24.522673230013975
25	24.492157667076874	25.24787641697265	27.4406614039827	22.81930451196777
26	24.617554953026044	25.2992359436646	26.424309731296297	23.65889937201306
27	24.508666086370713	25.213692316414704	26.49801398713344	23.779627610081143
28	23.828986116252622	25.337922340393597	26.52386050218945	24.309231041164328
29	24.90103286009198	25.282227269240646	25.68409889043412	24.13264098023325
30	24.734447901763232	25.035101234963165	26.809672933195934	23.420777930077673
31	24.771300029681804	24.61538718079554	26.361611088321713	24.251701701200943
32	23.09677935747231	24.770966526261727	27.026950411376472	25.105303704889494
33	23.425947233088877	24.86951678689465	27.502026033276973	24.202509946739504
34	23.75928390145641	24.75562536893816	26.780324632229103	24.70476609737633
35	24.07377762658956	25.549196757012744	26.448822232671997	23.928203383725698
36	24.606049085033366	24.922043575556867	26.541536183453562	23.930371155956205
37	24.648404019383218	24.834832431206582	26.41280386330362	24.10395968610658
38	24.15765373673907	25.17567292652586	26.189023068431567	24.477650268303503
39	23.684078880228917	24.648570771093258	26.895883567285985	24.771466781391844
40	23.74511000610311	25.5283527932579	27.206208499668165	23.52032870097083
41	24.326072963878246	24.537347380497387	26.729465360667277	24.407114294957093
42	23.512824874019085	25.796989798130383	26.91005746263928	23.78012786521126
43	24.362925091796818	24.775302070722734	26.689278198547928	24.172494638932523
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	29.0
2	51.0
3	200.5
4	350.0
5	350.0
6	470.0
7	590.0
8	593.5
9	597.0
10	768.0
11	939.0
12	939.0
13	1467.0
14	1995.0
15	3585.0
16	5175.0
17	5532.0
18	5889.0
19	5889.0
20	4566.0
21	3243.0
22	2573.5
23	1904.0
24	2402.0
25	2900.0
26	2900.0
27	3955.0
28	5010.0
29	6577.0
30	8144.0
31	10528.5
32	12913.0
33	12913.0
34	16567.5
35	20222.0
36	22458.0
37	24694.0
38	27936.5
39	31179.0
40	31179.0
41	35767.5
42	40356.0
43	43872.5
44	47389.0
45	49002.5
46	50616.0
47	50616.0
48	52564.5
49	54513.0
50	54888.5
51	55264.0
52	54304.5
53	53345.0
54	53345.0
55	49826.0
56	46307.0
57	41363.0
58	36419.0
59	33170.0
60	29921.0
61	29921.0
62	25744.5
63	21568.0
64	17286.0
65	13004.0
66	11418.0
67	9832.0
68	9832.0
69	8130.0
70	6428.0
71	5231.5
72	4035.0
73	3223.5
74	2412.0
75	2412.0
76	1715.0
77	1018.0
78	783.0
79	548.0
80	446.0
81	344.0
82	344.0
83	266.0
84	188.0
85	195.5
86	203.0
87	156.5
88	110.0
89	110.0
90	86.5
91	63.0
92	34.5
93	6.0
94	4.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	599694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.26961209465305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81652642598536	30.933717216330958
2	10.818848355055612	8.713416531379297
3	3.935078611709611	4.753922677665357
4	2.0242016617616145	3.260552628819693
5	1.282829986906476	2.5829532978056324
6	0.8523344042888998	2.0593905501384713
7	0.6035454576273459	1.7013179022100156
8	0.46189364115942816	1.4880222206781637
9	0.3535601802443921	1.281395817950156
>10	2.4185935735641486	18.907337351282376
>50	0.26617557227728256	7.62384278516676
>100	0.15467525379324654	12.185150870909846
>500	0.008802656719940861	2.2624236652417284
>1k	0.002934218906646954	2.2465564844215598
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4144	0.6910190864007311	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3073	0.5124280049491907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.25146157873848995	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1215	0.20260332769712555	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1199	0.19993530033650497	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1146	0.19109745970444925	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1024	0.17075375107971733	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	951	0.1585808762468859	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	916	0.15274456639552839	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	833	0.13890417446230913	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.12823206501982679	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	724	0.1207282380680814	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	686	0.1143916730866075	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	667	0.11122339059587057	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	661	0.11022288033563785	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	661	0.11022288033563785	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	624	0.10405306706420274	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	613	0.1022187982537761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0010005102602327186	0.0	0.0
2	0.0	0.0	0.0011672619702715052	0.0	0.0
3	0.0	0.0	0.001500765390349078	0.0	0.0
4	0.0	0.0	0.0023345239405430105	0.0	0.0
5	0.0	0.0	0.0031682824907369425	0.0	0.0
6	0.0	0.0	0.00750382695174539	0.0	0.0
7	0.0	0.0	0.014340647063335635	0.0	0.0
8	0.0	0.0	0.01700867442395622	0.0	0.0
9	0.0	0.0	0.020343708624731945	5.002551301163593E-4	0.0
10	8.337585501939323E-4	0.0	0.024012246245585248	5.002551301163593E-4	0.0
11	8.337585501939323E-4	0.0	0.03635187278845545	5.002551301163593E-4	0.0
12	8.337585501939323E-4	0.0	0.03985365869926996	5.002551301163593E-4	0.0
13	8.337585501939323E-4	0.0	0.043355444610084476	5.002551301163593E-4	0.0
14	8.337585501939323E-4	0.0	0.04652372710082142	6.670068401551458E-4	0.0
15	8.337585501939323E-4	0.0	0.051026023271868656	6.670068401551458E-4	0.0
16	8.337585501939323E-4	0.0	0.05502806431279953	6.670068401551458E-4	0.0
17	8.337585501939323E-4	0.0	0.055695071152954675	6.670068401551458E-4	0.0
18	8.337585501939323E-4	0.0	0.05736258825334254	6.670068401551458E-4	0.0
19	8.337585501939323E-4	0.0	0.05869660193365283	6.670068401551458E-4	0.0
20	8.337585501939323E-4	0.0	0.060364119034040695	6.670068401551458E-4	0.0
21	8.337585501939323E-4	0.0	0.06303214639466127	0.0010005102602327186	0.0
22	8.337585501939323E-4	0.0	0.06386590494485521	0.001500765390349078	0.0
23	8.337585501939323E-4	0.0	0.06469966349504915	0.0025012756505817966	0.0
24	8.337585501939323E-4	0.0	0.06536667033520428	0.003001530780698156	0.0
25	8.337585501939323E-4	0.0	0.06570017375528187	0.003335034200775729	0.0
26	8.337585501939323E-4	0.0	0.06586692546532065	0.0035017859108145153	0.0
27	8.337585501939323E-4	0.0	0.06603367717535943	0.00516930301120238	0.0
28	8.337585501939323E-4	0.0	0.06603367717535943	0.016675171003878646	0.0
29	8.337585501939323E-4	0.0	0.06603367717535943	0.04835799591124807	0.0
30	8.337585501939323E-4	0.0	0.06603367717535943	0.09338095762172041	0.0
31	8.337585501939323E-4	0.0	0.06603367717535943	0.19643351442569043	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCA	25	0.0054942737	29.599998	10
GGTATCA	680	0.0	27.477943	1
GTATCAA	2310	0.0	25.467533	1
CGTTATT	40	0.0019301162	23.125	10
AGAGCGA	40	0.0019301162	23.125	6
CGCCGTC	255	0.0	22.490196	26
GAGTCGC	245	0.0	21.89796	22
CGAGTCG	265	0.0	21.64151	21
CACATGT	105	9.804353E-10	21.142859	28
TGTTACG	45	0.0038237057	20.555557	16
GGACCGT	100	1.2867531E-8	20.349998	6
TAACTAG	100	1.2867531E-8	20.349998	26
GCCGTCC	255	0.0	20.313725	27
GCCGGCA	285	0.0	20.122808	15
TTCGCCG	295	0.0	20.067797	24
AGTCGCC	270	0.0	19.87037	23
TGTCCGG	330	0.0	19.621212	14
GTGACAC	105	2.2540917E-8	19.380953	24
CGCTCTC	315	0.0	19.38095	29
GGCGAGT	315	0.0	19.38095	19
>>END_MODULE