FastQCFastQC Report
Fri 10 Feb 2017
ERR1633060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644119
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27680.42973425717918584No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC23360.3626659049026655No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC22650.351643097005367No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG20570.3193509273907461No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA20480.3179536700516519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.3129856439570949No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG19660.3052231031843495No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG19660.3052231031843495No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC18850.29264778713250195No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT18350.2848852463597565No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG17520.2719994286769991No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA17110.2656341452433479No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC17070.26501314198152826No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT16990.263771135457889No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG16650.2584926077324221No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT14580.2263556889332561No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC14540.22573468567143648No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA14520.22542418404052667No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG14430.2240269267014325No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC13700.2126936171732242No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT13510.20974385167958093No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG13220.20524157803138862No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG12870.19980779949046681No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12650.19639228155045882No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC12640.19623703073500393No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC12310.19111375382499196No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA12300.19095850300953704No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG12170.18894024240862325No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT11890.18459321957588581No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT11550.17931469185041893No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA11410.17714118043405022No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC10870.16875763639948518No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT10740.16673937579857137No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG10430.16192660051946922No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10210.15851108257946125No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC10130.15726907605582197No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC10030.1557165679012729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9980.15494031382399837No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9610.14919603365216677No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG9450.14671202060488822No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA9170.1423649977721508No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA8930.138638978201233No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG8900.1381732257548683No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG8880.13786272412395847No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8830.13708647004668392No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT8730.13553396189213482No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8610.13367095210667593No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC8590.1333604504757661No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA8570.1330499488448563No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT8360.12978968172030325No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC8320.12916867845848362No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG8140.12637416378029526No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8060.125132157256656No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT8030.12466640481029127No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT8010.12435590317938146No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG7870.12218239176301274No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7870.12218239176301274No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7850.12187189013210292No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT7730.12000888034664403No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG7590.11783536893027531No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT7450.11566185751390658No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT7390.11473035262117713No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA7350.1141093493593575No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA7300.11333309528208298No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7250.11255684120480842No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7240.11240159038935352No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7170.11131483468116915No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT6890.10696781184843174No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC6890.10696781184843174No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG6570.10199978575387468No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT6500.10091303004569031No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC6460.10029202678387067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTC200.00184133537.014
CGCAAAG401.5986207E-632.37512
TGTCGAC250.005494571329.636
TCCGGGT250.005494571329.613
TCGAGCC652.9849616E-928.46153818
TTGTACT405.936136E-527.7500024
CTCGGGT405.936136E-527.75000222
CAAAGGC405.936136E-527.75000214
GGTATCA5600.027.751
TGGGCCA754.620233E-1027.13333329
CTTAATG358.8651455E-426.428572
TCGTTCG358.8651455E-426.4285723
CGTTCGG358.8651455E-426.4285724
CGGGGAC358.8651455E-426.4285716
GCCGAGC706.5811037E-926.4285722
TGACATT358.8651455E-426.428577
TTCGGTA358.8651455E-426.4285726
TAGGCAA358.8651455E-426.4285737
GTAAGCA358.8651455E-426.4285730
TTCGAGC706.5811037E-926.4285717
Produced by FastQC (version 0.11.5)