FastQCFastQC Report
Fri 10 Feb 2017
ERR1633059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423373
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9470.22367982842552547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.14880495449638972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450.026.4285741
GTAAGAC400.001929183523.1253
TACTTAG502.6981978E-422.1999997
ACCGCCT1301.8189894E-1221.34615312
GTATTAG1700.020.6764721
GTATCAA6000.020.6583331
ACCCGTC450.003821876620.55555524
TAACGGC1202.3646862E-1020.04166636
TTAACGG1202.3646862E-1020.04166635
TAGGACC1202.3646862E-1020.0416664
TATTAGA1850.020.02
TTAGGAC1303.274181E-1119.9230773
CGCCTGT759.243118E-619.73333410
ATTAGAG1457.2759576E-1219.137933
GGCACCG1306.9303496E-1018.59
GCACCGC1306.9303496E-1018.510
AACGGCC1306.9303496E-1018.537
GTCTTTA609.223685E-418.51
ATACTTA500.007027724318.4999986
CGCCGTT500.007027724318.49999825
Produced by FastQC (version 0.11.5)