##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423373
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731125981108857	33.0	33.0	33.0	27.0	33.0
2	31.251617368136372	33.0	33.0	33.0	27.0	33.0
3	31.676590146277633	33.0	33.0	33.0	27.0	33.0
4	31.81328048789129	33.0	33.0	33.0	27.0	33.0
5	31.924340947580504	33.0	33.0	33.0	33.0	33.0
6	34.81336079532705	37.0	37.0	37.0	27.0	37.0
7	34.98404244011782	37.0	37.0	37.0	33.0	37.0
8	35.15809227324369	37.0	37.0	37.0	33.0	37.0
9	35.22887855389928	37.0	37.0	37.0	33.0	37.0
10	35.24483138981466	37.0	37.0	37.0	33.0	37.0
11	35.26762925363686	37.0	37.0	37.0	33.0	37.0
12	35.23595741816318	37.0	37.0	37.0	33.0	37.0
13	35.26499091817381	37.0	37.0	37.0	33.0	37.0
14	35.23937757013319	37.0	37.0	37.0	33.0	37.0
15	35.265959331369736	37.0	37.0	37.0	33.0	37.0
16	35.27677249139648	37.0	37.0	37.0	33.0	37.0
17	35.23396626615301	37.0	37.0	37.0	33.0	37.0
18	35.23728012886981	37.0	37.0	37.0	33.0	37.0
19	35.23835246933555	37.0	37.0	37.0	33.0	37.0
20	35.23168459018407	37.0	37.0	37.0	33.0	37.0
21	35.24183403287409	37.0	37.0	37.0	33.0	37.0
22	35.24658161951754	37.0	37.0	37.0	33.0	37.0
23	35.25043873841742	37.0	37.0	37.0	33.0	37.0
24	35.225619016800785	37.0	37.0	37.0	33.0	37.0
25	35.22162017889662	37.0	37.0	37.0	33.0	37.0
26	35.18927281616919	37.0	37.0	37.0	33.0	37.0
27	35.13438740779407	37.0	37.0	37.0	33.0	37.0
28	35.09836243690552	37.0	37.0	37.0	33.0	37.0
29	35.12625273694827	37.0	37.0	37.0	33.0	37.0
30	35.0814837034955	37.0	37.0	37.0	33.0	37.0
31	35.09280941392106	37.0	37.0	37.0	33.0	37.0
32	35.10760960193494	37.0	37.0	37.0	33.0	37.0
33	35.10832764488997	37.0	37.0	37.0	33.0	37.0
34	35.03952306831092	37.0	37.0	37.0	27.0	37.0
35	35.040694612079655	37.0	37.0	37.0	27.0	37.0
36	35.01854156972693	37.0	37.0	37.0	27.0	37.0
37	35.049200114319994	37.0	37.0	37.0	27.0	37.0
38	34.90009518793121	37.0	37.0	37.0	27.0	37.0
39	34.98012627163281	37.0	37.0	37.0	27.0	37.0
40	35.005560108934674	37.0	37.0	37.0	27.0	37.0
41	35.02924844050046	37.0	37.0	37.0	27.0	37.0
42	34.961329607698175	37.0	37.0	37.0	27.0	37.0
43	34.753642768905905	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	15.0
17	19.0
18	13.0
19	12.0
20	28.0
21	80.0
22	250.0
23	580.0
24	1214.0
25	2152.0
26	3451.0
27	5185.0
28	7425.0
29	10013.0
30	13005.0
31	16508.0
32	21127.0
33	27485.0
34	39621.0
35	71696.0
36	203485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62257158581204	20.333370337740007	13.590380113989319	25.453677962458638
2	15.826233604882692	22.507103665089648	38.15122834946962	23.51543438055804
3	17.708970576772728	26.62687511957541	29.871531722618116	25.792622581033747
4	12.87658872908759	17.93501238860296	38.90399246054897	30.28440642176048
5	14.123479768431146	36.79379648678589	35.355820990001725	13.726902754781245
6	30.023407255540626	39.47795442789219	17.42742215493194	13.071216161635249
7	26.98589659709051	31.756630677912856	23.604481154915405	17.652991570081227
8	24.351340307483	36.45910343833924	20.44202157435642	18.74753467982134
9	25.827107538742432	15.731045673673098	20.224719101123593	38.21712768646087
10	14.94733957999211	28.189799538468442	33.920207476622274	22.942653404917177
11	34.43511985884788	23.436780333181378	24.007671722098483	18.12042808587227
12	23.109645631629792	25.427696145006884	30.321489561214342	21.141168662148978
13	27.942972272676812	21.979200374138173	26.03141910324938	24.046408249935634
14	22.08478103232847	20.578544215148344	27.539309308812797	29.797365443710394
15	23.8739834613922	28.67778530988041	23.958070070599685	23.490161158127705
16	23.39591802027999	28.299631766787208	24.814997649826513	23.48945256310629
17	22.714958204703652	27.564582531243136	26.35359363965109	23.366865624402124
18	23.063350756897584	27.40680203980887	27.24358898654378	22.28625821674977
19	24.735162610747498	26.342492317648976	26.741195116363116	22.181149955240414
20	24.282370392065626	26.33517016909439	26.951175441041354	22.43128399779863
21	23.4254428128388	26.9003927978402	26.85527891481034	22.818885474510655
22	23.04067571621242	26.69867941507843	26.991092960580858	23.269551908128292
23	23.04232910459571	26.542316113686987	27.380584024016645	23.034770757700656
24	23.228925793567374	27.121946841201495	26.12920521620415	23.519922149026982
25	23.55606049511896	26.65403792872951	26.90865973975667	22.881241836394857
26	23.379620334787525	26.95164783772229	26.927319408653833	22.741412418836347
27	23.197275215944334	26.997942712454503	26.420910166685168	23.383871904915996
28	23.017055882165373	26.715449497251832	27.16776931925276	23.099725301330032
29	24.115850562033952	26.603255285528366	26.509720742702065	22.771173409735624
30	23.67368726867325	26.510429337723473	26.756784206834162	23.059099186769114
31	23.503152066853577	26.589791980121547	26.777805859136034	23.12925009388884
32	22.736452253686465	26.530033799982522	27.046363372250948	23.687150574080064
33	22.873211092818863	26.54727627883686	27.426642700408387	23.15286992793589
34	23.478351241104182	26.23336868435162	27.14580287358901	23.142477200955184
35	23.28773918034452	26.928500400356185	26.8172509819946	22.966509437304694
36	23.842096685428686	26.74898966159864	26.378394465400483	23.03051918757219
37	23.352929922314367	26.23053430426598	26.665139250731624	23.751396522688033
38	23.444574878416905	26.537592146877575	26.808275445056722	23.209557529648798
39	23.109645631629792	26.54987446058204	27.046599570591418	23.29388033719675
40	23.120274556950964	26.818904370377894	27.211938408920737	22.848882663750405
41	23.25183703259301	26.12826042284227	27.09289444532363	23.527008099241094
42	22.756765310966927	26.788907181138143	27.04305659548436	23.41127091241057
43	23.377494549723295	25.868914645005702	27.08722568515234	23.666365120118666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	11.0
2	19.0
3	66.0
4	113.0
5	113.0
6	121.0
7	129.0
8	133.5
9	138.0
10	188.5
11	239.0
12	239.0
13	345.0
14	451.0
15	825.0
16	1199.0
17	1479.5
18	1760.0
19	1760.0
20	1798.5
21	1837.0
22	2254.0
23	2671.0
24	3749.5
25	4828.0
26	4828.0
27	6435.0
28	8042.0
29	10044.5
30	12047.0
31	14621.0
32	17195.0
33	17195.0
34	19903.0
35	22611.0
36	24799.0
37	26987.0
38	29086.5
39	31186.0
40	31186.0
41	32408.0
42	33630.0
43	33988.5
44	34347.0
45	33828.5
46	33310.0
47	33310.0
48	32530.0
49	31750.0
50	31129.5
51	30509.0
52	28978.5
53	27448.0
54	27448.0
55	26431.5
56	25415.0
57	22546.0
58	19677.0
59	18210.0
60	16743.0
61	16743.0
62	14796.0
63	12849.0
64	10883.0
65	8917.0
66	7668.0
67	6419.0
68	6419.0
69	5308.0
70	4197.0
71	3446.0
72	2695.0
73	2187.0
74	1679.0
75	1679.0
76	1321.0
77	963.0
78	772.0
79	581.0
80	448.0
81	315.0
82	315.0
83	256.5
84	198.0
85	173.5
86	149.0
87	114.5
88	80.0
89	80.0
90	58.0
91	36.0
92	21.5
93	7.0
94	5.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423373.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.87980407403836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79813942838685	64.54672668325625
2	6.011607664168395	8.642263621408231
3	1.5359061830911067	3.3120190655007846
4	0.7679255075985193	2.2079334011855205
5	0.39955036640184116	1.435980102733729
6	0.27606475257463914	1.190608819608776
7	0.19233955361598376	0.9677730600723435
8	0.14784383157172262	0.8501588517544437
9	0.11305009937636944	0.7313417094351601
>10	0.6680979184647488	9.244251000647003
>50	0.06009001644469291	2.9276029765406544
>100	0.02872434845556493	3.569084069900308
>500	6.603298495532168E-4	0.3742566379568313
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.22367982842552547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	630	0.14880495449638972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	2.3619834047045987E-4	0.0	0.0
7	0.0	0.0	0.001653388383293219	0.0	0.0
8	0.0	0.0	0.0021257850642341387	0.0	0.0
9	0.0	0.0	0.0023619834047045986	0.0	0.0
10	0.0	0.0	0.0025981817451750586	2.3619834047045987E-4	0.0
11	0.0	0.0	0.003306776766586438	2.3619834047045987E-4	0.0
12	0.0	0.0	0.003306776766586438	2.3619834047045987E-4	0.0
13	0.0	0.0	0.003779173447527358	2.3619834047045987E-4	0.0
14	0.0	0.0	0.005196363490350117	2.3619834047045987E-4	0.0
15	0.0	0.0	0.005904958511761496	2.3619834047045987E-4	0.0
16	0.0	0.0	0.006613553533172876	2.3619834047045987E-4	0.0
17	0.0	0.0	0.006613553533172876	2.3619834047045987E-4	0.0
18	0.0	0.0	0.006613553533172876	2.3619834047045987E-4	0.0
19	0.0	0.0	0.007085950214113796	2.3619834047045987E-4	0.0
20	0.0	0.0	0.007322148554584256	4.7239668094091973E-4	0.0
21	0.0	0.0	0.007794545235525175	4.7239668094091973E-4	0.0
22	0.0	0.0	0.007794545235525175	9.447933618818395E-4	0.0
23	0.0	0.0	0.007794545235525175	9.447933618818395E-4	0.0
24	0.0	0.0	0.007794545235525175	9.447933618818395E-4	0.0
25	0.0	0.0	0.007794545235525175	0.0011809917023522993	0.0
26	0.0	0.0	0.007794545235525175	0.001417190042822759	0.0
27	0.0	0.0	0.007794545235525175	0.003070578426115978	0.0
28	0.0	0.0	0.007794545235525175	0.008266941916466096	0.0
29	0.0	0.0	0.007794545235525175	0.01606148715199127	0.0
30	0.0	0.0	0.007794545235525175	0.029288594218337023	0.0
31	0.0	0.0	0.007794545235525175	0.08077983244089727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	245	0.0	26.428574	1
GTAAGAC	40	0.0019291835	23.125	3
TACTTAG	50	2.6981978E-4	22.199999	7
ACCGCCT	130	1.8189894E-12	21.346153	12
GTATTAG	170	0.0	20.676472	1
GTATCAA	600	0.0	20.658333	1
ACCCGTC	45	0.0038218766	20.555555	24
TAACGGC	120	2.3646862E-10	20.041666	36
TTAACGG	120	2.3646862E-10	20.041666	35
TAGGACC	120	2.3646862E-10	20.041666	4
TATTAGA	185	0.0	20.0	2
TTAGGAC	130	3.274181E-11	19.923077	3
CGCCTGT	75	9.243118E-6	19.733334	10
ATTAGAG	145	7.2759576E-12	19.13793	3
GGCACCG	130	6.9303496E-10	18.5	9
GCACCGC	130	6.9303496E-10	18.5	10
AACGGCC	130	6.9303496E-10	18.5	37
GTCTTTA	60	9.223685E-4	18.5	1
ATACTTA	50	0.0070277243	18.499998	6
CGCCGTT	50	0.0070277243	18.499998	25
>>END_MODULE