FastQCFastQC Report
Fri 10 Feb 2017
ERR1633058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483140
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13860.2868733700376702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.20987705426998385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5090.10535248582191498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT250.00549322929.66
GGTATCA2350.025.191491
GTATCAA9000.025.0777781
TATCGCG601.3347271E-624.6666667
ATCGCGC652.6775033E-622.769238
ACGTTTA450.003822646620.5555551
CGGGAAC450.003822646620.55555517
CGGGTAA609.226255E-418.523
TCCTATA500.00702912418.52
CCGCGAT500.00702912418.532
TCGCGCG609.226255E-418.59
ACGCTTC1054.783051E-717.61904725
TGTATCG852.7178288E-517.4117645
GTATCGC852.7178288E-517.4117646
TATCAAC13050.017.1532572
ACGGGTA650.001578142217.07692316
CTTATAT650.001578142217.0769231
CGTTTAC650.001578142217.0769232
TCAACGC13450.016.780674
ATCAACG13500.016.7185173
Produced by FastQC (version 0.11.5)