FastQCFastQC Report
Fri 10 Feb 2017
ERR1633057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606278
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40470.6675155621678505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.4849260570233457No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17580.2899659891996741No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16760.2764408406704515No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG16000.2639053371555623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15440.25466865035511765No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC15110.24922560277628414No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT15030.24790607609050633No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT14860.2451020818832285No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA14320.23619527675422824No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13970.23042234750395033No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13930.22976258416106143No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13840.2282781166395614No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG13800.2276183532966725No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13090.21590755396039438No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12990.21425814560317213No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT11930.19677441701661616No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11490.18951702024483816No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT11370.18753773021617146No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10890.1796205701015046No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10700.1764866942227823No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10480.17285799583689332No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC10460.17252811416544883No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10420.17186835082255997No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA10410.17170340998683772No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10400.17153846915111548No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG10360.1708787058082266No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT10270.16939423828672656No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9950.1641161315436153No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9700.15999261065055964No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9640.1590029656362263No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG9630.15883802480050407No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9360.15438462223600394No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC9130.15059098301439272No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9120.1504260421786705No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9040.14910651549289272No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT8960.14778698880711488No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8900.14679734379278153No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7940.13096302356344777No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC7900.13030326022055888No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7860.12964349687767No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA7840.12931361520622553No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7740.12766420684900326No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC7590.12519009431316985No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7470.12321080428450315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.1215613959272809No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7360.12139645509155865No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG7330.12090163258439199No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7330.12090163258439199No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG7220.1190872833914475No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT7150.11793269754139189No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7090.11694305252705854No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT6580.108531069905225No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6560.10820118823378054No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG6320.1042426081764471No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA6310.10407766734072488No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6240.1029230814906693No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT6220.10259319981922484No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6220.10259319981922484No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT6200.10226331814778039No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6180.10193343647633595No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6180.10193343647633595No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6150.10143861396916926No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6150.10143861396916926No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6150.10143861396916926No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA6070.10011908728339144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGGG200.001841249637.011
CGGCTGG303.5974896E-430.83333422
GCTGGGG250.005494319429.633
TAGGTTG250.005494319429.69
TCGTAGG250.005494319429.62
GCTAGAA250.005494319429.62
TCCTAAG454.0041486E-628.7777792
TAAGGTA454.0041486E-628.7777795
ATAGATG652.9849616E-928.4615386
GGTATCA7800.028.224361
ACTAGTG358.864544E-426.428578
TAGATGG509.081836E-625.9000027
TCGGGTA451.3222043E-424.66666825
CCCCAGA451.3222043E-424.66666836
AGGACCG751.3709723E-824.6666645
GTATCAA24200.023.9276851
CGTTATT551.9007362E-523.54545410
GGACCGT1103.6379788E-1223.5454546
TAGCGAA400.001930139723.12500210
GCGTTAG400.001930139723.12500221
Produced by FastQC (version 0.11.5)