FastQCFastQC Report
Fri 10 Feb 2017
ERR1633056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357993
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101542.8363683088775478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77922.176578871653915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42191.1785146636945414No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG20970.5857656434623023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.5528041051082004No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15910.4444220976387806No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG15420.43073467916970437No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14580.4072705332227166No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14330.4002871564527798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.3047545622400438No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10460.2921844840541575No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10230.2857597774258156No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9250.2583849404876632No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9170.2561502599212834No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8780.2452561921601819No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8160.22793741777073856No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7810.21816069029282695No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7620.21285332394767492No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7510.20978063816890274No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7490.2092219680273078No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7460.2083839628149154No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7150.1997245756201937No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6980.19497587941663663No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6940.19385853913344675No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6900.19274119885025684No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6880.1921825287086619No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6690.1868751623635099No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6670.18631649222191496No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6660.18603715715111746No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.18324380644314275No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6470.1807297908059655No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6440.17989178559357305No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6410.17905378038118064No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6330.17681909981480085No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG6260.17486375431921855No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6200.1731877438944337No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6180.17262907375283876No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6170.1723497386820413No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6080.16983572304486402No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5960.16648370219529432No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5770.16117633585014232No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5690.15894165528376253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.15251694865542062No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5420.15139960837223074No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5420.15139960837223074No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5380.15028226808904085No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5280.1474889173810661No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5220.14581290695628127No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5160.14413689653149642No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5110.14274022117750906No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4950.13827086004474948No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4930.1377121899031545No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4920.13743285483235707No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4830.1349188391951798No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4830.1349188391951798No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4820.13463950412438233No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.13044947806242022No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4630.1293321377792303No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4580.12793546242524295No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4570.12765612735444548No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4570.12765612735444548No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4530.1265387870712556No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4520.12625945200045813No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4510.12598011692966063No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4470.12486277664647075No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4380.12234876100929348No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG4360.12179009086769853No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4320.12067275058450865No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4290.11983474537211622No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.1189967401597238No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4230.11815873494733138No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4210.11760006480573643No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4190.11704139466414147No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG4190.11704139466414147No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4080.11396870888536928No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4070.11368937381457178No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4070.11368937381457178No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4060.11341003874377432No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3860.10782333732782484No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3850.10754400225702737No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3850.10754400225702737No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3770.10530932169064758No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3760.10502998661985011No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3750.10475065154905262No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3690.10307464112426779No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3690.10307464112426779No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.10111929562868548No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3620.10111929562868548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGA200.001840243737.020
TTGTACT200.001840243737.04
GGATATA200.001840243737.02
GAATCAA1000.031.451
CACGGCG250.005491351229.619
TTTGTAC250.005491351229.63
CTCCCAT250.005491351229.61
TAAGTTC250.005491351229.630
CACACGG250.005491351229.617
TGCACTA250.005491351229.65
CTAGTGC604.305366E-827.7500029
TTGCACT405.928883E-527.754
ACTAGTG1100.026.909098
CCGATGG358.857419E-426.4285737
CTAGTGG358.857419E-426.428579
GGTATCA15250.025.8393441
TAGTGCA659.354153E-825.61538510
CTGCTCG5350.023.5140199
GTATCAA41300.023.0690081
ACTGCTC5500.022.8727268
Produced by FastQC (version 0.11.5)