FastQCFastQC Report
Fri 10 Feb 2017
ERR1633055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521591
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24900.4773855377105817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.33915462498394333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9350.17925922801582084No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG8100.15529409058055066No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7940.15222655298883608No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7520.1441742668105853No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG6720.1288365788520124No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC5720.10966446890379626No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5370.10295423042192063No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC5270.10103701942709901No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA5250.1006535772281347No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT5240.10046185612865252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.026.7222231
CCGTCTA358.862873E-426.4285722
GTATCAA15000.025.5299991
CGAGTCG451.3218586E-424.66666621
GAGTCGC451.3218586E-424.66666622
GTCCGGC701.9187428E-723.78571515
TTAACGG1201.0913936E-1121.58333235
GGCGAGT603.7212656E-521.58333219
CCCGACA450.003823048220.55555532
GGCGATA555.1379186E-420.1818186
TAAAGCA656.892892E-519.9230774
CCGCTCT851.2426408E-619.58823628
GCCGCTC851.2426408E-619.58823627
CGCTCTC851.2426408E-619.58823629
GGTCGCC1052.2522727E-819.3809536
AACGGCC1355.638867E-1119.18518437
ATTACGC500.00702985518.53
TAACGGC1409.276846E-1118.536
ACGATTA801.6139133E-518.523
GCGATAG609.227599E-418.57
Produced by FastQC (version 0.11.5)