##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521591
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.721034296987487	33.0	33.0	33.0	27.0	33.0
2	31.258047013848014	33.0	33.0	33.0	27.0	33.0
3	31.673982104752575	33.0	33.0	33.0	27.0	33.0
4	31.800989664315527	33.0	33.0	33.0	27.0	33.0
5	31.912657618708913	33.0	33.0	33.0	33.0	33.0
6	34.77084727305494	37.0	37.0	37.0	27.0	37.0
7	34.89668533391105	37.0	37.0	37.0	27.0	37.0
8	35.10437488376908	37.0	37.0	37.0	33.0	37.0
9	35.159941410032	37.0	37.0	37.0	33.0	37.0
10	35.20498819956633	37.0	37.0	37.0	33.0	37.0
11	35.22704379485076	37.0	37.0	37.0	33.0	37.0
12	35.14978210897044	37.0	37.0	37.0	33.0	37.0
13	35.2030729057825	37.0	37.0	37.0	33.0	37.0
14	35.160397706248766	37.0	37.0	37.0	33.0	37.0
15	35.22139377404901	37.0	37.0	37.0	33.0	37.0
16	35.22211847980506	37.0	37.0	37.0	33.0	37.0
17	35.186648159189865	37.0	37.0	37.0	33.0	37.0
18	35.15722663926333	37.0	37.0	37.0	33.0	37.0
19	35.196815129095405	37.0	37.0	37.0	33.0	37.0
20	35.10837418590428	37.0	37.0	37.0	33.0	37.0
21	35.17320275848318	37.0	37.0	37.0	33.0	37.0
22	35.196715434123675	37.0	37.0	37.0	33.0	37.0
23	35.165108293663046	37.0	37.0	37.0	33.0	37.0
24	35.161089819417896	37.0	37.0	37.0	33.0	37.0
25	35.17940301884043	37.0	37.0	37.0	33.0	37.0
26	35.13633862547475	37.0	37.0	37.0	33.0	37.0
27	35.06279441171339	37.0	37.0	37.0	33.0	37.0
28	35.06610160067946	37.0	37.0	37.0	27.0	37.0
29	35.085106913271126	37.0	37.0	37.0	33.0	37.0
30	35.052675372082724	37.0	37.0	37.0	27.0	37.0
31	35.033278948448114	37.0	37.0	37.0	27.0	37.0
32	35.06692983582922	37.0	37.0	37.0	27.0	37.0
33	35.04533437118355	37.0	37.0	37.0	27.0	37.0
34	34.98693804149228	37.0	37.0	37.0	27.0	37.0
35	34.96657534351628	37.0	37.0	37.0	27.0	37.0
36	34.94876445337439	37.0	37.0	37.0	27.0	37.0
37	34.96390850302248	37.0	37.0	37.0	27.0	37.0
38	34.82601885385292	37.0	37.0	37.0	27.0	37.0
39	34.8875229825668	37.0	37.0	37.0	27.0	37.0
40	34.92643853133969	37.0	37.0	37.0	27.0	37.0
41	34.946975695516215	37.0	37.0	37.0	27.0	37.0
42	34.803230883968475	37.0	37.0	37.0	27.0	37.0
43	34.63422298314197	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	13.0
16	19.0
17	23.0
18	26.0
19	17.0
20	41.0
21	119.0
22	289.0
23	788.0
24	1558.0
25	2672.0
26	4551.0
27	6908.0
28	9434.0
29	13018.0
30	16706.0
31	21158.0
32	26614.0
33	34895.0
34	49182.0
35	88414.0
36	245143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16637173570863	20.15832328395237	13.508668669513085	24.166636310825915
2	16.257565793888315	23.589172359185646	38.143104463075474	22.010157383850565
3	18.053225611638236	27.062775239603443	30.2112191352995	24.67278001345882
4	12.291047966701878	18.131064378027993	39.59232425406113	29.985563401208992
5	14.249095555713193	36.50868208999005	34.911261889104686	14.330960465192074
6	29.131254181916482	40.4180670295308	17.172267159517705	13.278411629035011
7	27.135245815207703	32.174251472897346	23.002697515869713	17.687805196025238
8	24.490836690050248	35.53205480922792	20.72255847972837	19.25455002099346
9	26.50275790801605	15.065443997308236	20.178645720497478	38.253152374178235
10	15.243936340926128	28.019655247118912	33.799471233207626	22.936937178747332
11	34.36140577578984	24.147655921977183	23.42908524111804	18.061853061114935
12	23.53280635593789	25.72130270652676	29.937633126338454	20.808257811196896
13	28.336378503463443	22.436928551297854	26.180474739786536	23.046218205452167
14	22.67236206146195	21.189782799166395	27.656727205799182	28.481127933572477
15	23.783577554060557	28.799768400911823	25.185058791275157	22.231595253752463
16	23.420266070541864	28.498957995824313	24.896326815455023	23.184449118178804
17	23.085137588647044	26.847664165984458	25.748910544852194	24.318287700516304
18	23.488327060858026	26.280553153716223	27.931463541357115	22.29965624406863
19	25.94906737271157	25.885607688782976	27.07159441017962	21.093730528325832
20	25.20077992143269	26.08576451664235	27.120866732746542	21.592588829178418
21	23.223943664672127	27.180683715784976	27.03037437379096	22.56499824575194
22	23.577669093216716	26.089215496433027	27.402313306786354	22.930802103563906
23	22.626732439785197	26.59555092016542	28.16325435063105	22.614462289418338
24	23.06002212461488	27.356683685109594	26.45137665335483	23.13191753692069
25	23.19211796215809	27.084439723844927	27.82007358255798	21.903368731439002
26	24.032239820088922	26.70272301477595	26.591333055976808	22.673704109158326
27	23.724527455420052	26.46307164042324	26.51790387487514	23.294497029281562
28	22.899359843248828	26.367019369582678	27.840587740202572	22.89303304696592
29	24.003098212967632	26.944099879023987	26.99893211347588	22.0538697945325
30	23.94979974731159	26.254862526385615	26.974391812742166	22.820945913560625
31	23.027621258802395	26.209999789106792	27.417075831446482	23.34530312064434
32	22.31767802741995	26.5226969023622	27.37029588317283	23.789329187045023
33	22.792571190837265	26.143664288685965	28.031541955286805	23.032222565189965
34	23.463978481223794	26.076178461668242	27.77214330768744	22.687699749420524
35	23.337250834466087	26.950810117505863	26.680099925037048	23.031839122991002
36	23.150897925769424	26.74125895577186	26.606670743935386	23.501172374523332
37	23.299865220067066	26.331934408377442	27.423402627729388	22.9447977438261
38	23.143804245088585	26.017128363027737	26.96825673755874	23.870810654324938
39	22.714540703348028	26.245659913610474	27.807803432191125	23.23199595085038
40	23.24273233242138	26.75563803823302	27.455036609143946	22.54659302020165
41	23.325747568497157	26.016361478629808	27.38908455092208	23.268806401950954
42	22.588579940988247	27.460213078829966	27.039960428765063	22.911246551416724
43	23.812527440082363	25.498906231127457	27.09517610541593	23.593390223374254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	28.5
2	44.0
3	159.5
4	275.0
5	275.0
6	347.5
7	420.0
8	387.0
9	354.0
10	473.5
11	593.0
12	593.0
13	914.5
14	1236.0
15	2227.5
16	3219.0
17	3565.5
18	3912.0
19	3912.0
20	3507.0
21	3102.0
22	3425.0
23	3748.0
24	4947.0
25	6146.0
26	6146.0
27	7787.0
28	9428.0
29	11595.0
30	13762.0
31	16494.0
32	19226.0
33	19226.0
34	23859.5
35	28493.0
36	30869.5
37	33246.0
38	35136.0
39	37026.0
40	37026.0
41	37799.5
42	38573.0
43	39977.0
44	41381.0
45	40280.5
46	39180.0
47	39180.0
48	37917.5
49	36655.0
50	37337.0
51	38019.0
52	37957.5
53	37896.0
54	37896.0
55	36053.0
56	34210.0
57	29319.5
58	24429.0
59	22323.5
60	20218.0
61	20218.0
62	18214.5
63	16211.0
64	12555.0
65	8899.0
66	7746.5
67	6594.0
68	6594.0
69	5707.5
70	4821.0
71	4707.0
72	4593.0
73	4118.5
74	3644.0
75	3644.0
76	2200.0
77	756.0
78	614.0
79	472.0
80	377.5
81	283.0
82	283.0
83	230.5
84	178.0
85	178.0
86	178.0
87	141.0
88	104.0
89	104.0
90	72.0
91	40.0
92	25.5
93	11.0
94	6.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	521591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.75498238074388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57887834950228	53.52784297461308
2	5.755714543258621	6.878652422420204
3	1.468819275417943	2.6330780976928856
4	0.6984053867816828	1.669328063270243
5	0.4633561626966062	1.38439196689724
6	0.2911880247890357	1.0439961174451464
7	0.22559528562798503	0.9436309622515391
8	0.16439916406705474	0.7858935321788703
9	0.13463125086000405	0.7240399220733347
>10	0.9816685764163884	11.71064851291191
>50	0.13239596222327124	5.489490161800223
>100	0.10075009762471393	11.006959338249155
>500	0.0035520867752302987	1.3802356142996721
>1k	6.458339591327817E-4	0.8218123138965894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2490	0.4773855377105817	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1769	0.33915462498394333	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.17925922801582084	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	810	0.15529409058055066	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	794	0.15222655298883608	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	752	0.1441742668105853	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	672	0.1288365788520124	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	572	0.10966446890379626	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	537	0.10295423042192063	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	527	0.10103701942709901	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	525	0.1006535772281347	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	524	0.10046185612865252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.917210994821613E-4	0.0	0.0
2	0.0	0.0	1.917210994821613E-4	1.917210994821613E-4	0.0
3	0.0	0.0	3.834421989643226E-4	3.834421989643226E-4	0.0
4	0.0	0.0	5.75163298446484E-4	3.834421989643226E-4	0.0
5	0.0	0.0	0.001150326596892968	3.834421989643226E-4	0.0
6	0.0	0.0	0.002300653193785936	3.834421989643226E-4	0.0
7	0.0	0.0	0.006901959581357807	5.75163298446484E-4	0.0
8	0.0	0.0	0.00728540178032213	5.75163298446484E-4	0.0
9	1.917210994821613E-4	0.0	0.009010891675661582	0.001150326596892968	0.0
10	3.834421989643226E-4	0.0	0.009969497173072389	0.001150326596892968	0.0
11	3.834421989643226E-4	0.0	0.01763834115235884	0.001342047696375129	0.0
12	3.834421989643226E-4	0.0	0.019555552147180452	0.0015337687958572905	0.0
13	3.834421989643226E-4	0.0	0.02070587874407342	0.0015337687958572905	0.0
14	3.834421989643226E-4	0.0	0.022239647539930713	0.0015337687958572905	0.0
15	3.834421989643226E-4	0.0	0.02530718513164529	0.0015337687958572905	0.0
16	3.834421989643226E-4	0.0	0.02760783832543123	0.0015337687958572905	0.0
17	3.834421989643226E-4	0.0	0.02779955942491339	0.0015337687958572905	0.0
18	3.834421989643226E-4	0.0	0.028183001623877713	0.0015337687958572905	0.0
19	3.834421989643226E-4	0.0	0.028949886021806356	0.0015337687958572905	0.0
20	3.834421989643226E-4	0.0	0.02914160712128852	0.0017254898953394519	0.0
21	3.834421989643226E-4	0.0	0.03067537591714581	0.0017254898953394519	0.0
22	3.834421989643226E-4	0.0	0.031250539215592295	0.0021089320943037744	0.0
23	3.834421989643226E-4	0.0	0.03163398141455662	0.003067537591714581	0.0
24	3.834421989643226E-4	0.0	0.03163398141455662	0.003642700890161065	0.0
25	3.834421989643226E-4	0.0	0.03163398141455662	0.003834421989643226	0.0
26	3.834421989643226E-4	0.0	0.03163398141455662	0.004026143089125388	0.0
27	3.834421989643226E-4	0.0	0.03163398141455662	0.005751632984464839	0.0
28	3.834421989643226E-4	0.0	0.031825702514038774	0.012078429267376163	0.0
29	3.834421989643226E-4	0.0	0.031825702514038774	0.02914160712128852	0.0
30	3.834421989643226E-4	0.0	0.031825702514038774	0.04869715926846897	0.0
31	3.834421989643226E-4	0.0	0.031825702514038774	0.12174289817117243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	450	0.0	26.722223	1
CCGTCTA	35	8.862873E-4	26.428572	2
GTATCAA	1500	0.0	25.529999	1
CGAGTCG	45	1.3218586E-4	24.666666	21
GAGTCGC	45	1.3218586E-4	24.666666	22
GTCCGGC	70	1.9187428E-7	23.785715	15
TTAACGG	120	1.0913936E-11	21.583332	35
GGCGAGT	60	3.7212656E-5	21.583332	19
CCCGACA	45	0.0038230482	20.555555	32
GGCGATA	55	5.1379186E-4	20.181818	6
TAAAGCA	65	6.892892E-5	19.923077	4
CCGCTCT	85	1.2426408E-6	19.588236	28
GCCGCTC	85	1.2426408E-6	19.588236	27
CGCTCTC	85	1.2426408E-6	19.588236	29
GGTCGCC	105	2.2522727E-8	19.38095	36
AACGGCC	135	5.638867E-11	19.185184	37
ATTACGC	50	0.007029855	18.5	3
TAACGGC	140	9.276846E-11	18.5	36
ACGATTA	80	1.6139133E-5	18.5	23
GCGATAG	60	9.227599E-4	18.5	7
>>END_MODULE