FastQCFastQC Report
Fri 10 Feb 2017
ERR1633054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532235
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26410.4962093811943972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.36637951280919145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9830.18469285184176162No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7830.1471154659126138No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC6100.1146110270839009No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6030.11329581857638074No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5390.10127105507905343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCC250.005493724329.631
GGTATCA4150.027.6385541
GCCGTCC856.91216E-1126.11764727
CCGTCCG901.4188117E-1024.66666828
GTATCAA13650.024.6666661
CGAGCCC400.001929832423.12515
ATGCGTT400.001929832423.12536
TGCGTTA400.001929832423.12537
GCCGGCA1155.456968E-1222.5217415
CTAGAAC502.699461E-422.23
CGTCCGC1005.329639E-1022.229
CGGCCTT603.7214493E-521.58333224
CCGGCAG1301.8189894E-1221.34615316
CGCCGTC1059.786163E-1021.14285726
AATACTG1950.019.9230775
TCCGGCG1153.0395313E-919.30434816
AGCTTCG1153.0395313E-919.30434821
TTCGCCG1153.0395313E-919.30434824
GCCGCTC1254.110916E-1019.24000227
CGAGTCG1103.8400685E-818.521
Produced by FastQC (version 0.11.5)