FastQCFastQC Report
Fri 10 Feb 2017
ERR1633053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594122
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25020.4211256273963933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.30852249201342485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9330.1570384533816287No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6520.10974177020881233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT200.001841220737.010
GGTATCA6700.024.0223871
GTATCAA16250.021.9723071
TCACGGC450.003823665420.55555533
CTAGACC450.003823665420.5555553
TTAAGCA450.003823665420.5555554
TTTAGGC1002.8691102E-718.53
CGAGTCG1501.2732926E-1118.521
ATTCACG500.007030979318.531
CGGGATA500.007030979318.516
GTCCGGC1700.018.515
ACGTCGC500.007030979318.516
TGTCCGG1800.018.514
CCGTCCG1351.1477823E-917.81481628
TTAGGCC953.6004549E-617.5263164
GACCCAT852.7195309E-517.4117646
GGCGAGT1705.456968E-1217.41176419
GCGAGTC1603.45608E-1117.3437520
TTCGCCG1502.5102054E-1017.26666624
CGTCCGC1401.8626451E-917.17857229
Produced by FastQC (version 0.11.5)