##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633053.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594122
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814440468455974	33.0	33.0	33.0	33.0	33.0
2	31.32150467412417	33.0	33.0	33.0	27.0	33.0
3	31.75520179357102	33.0	33.0	33.0	27.0	33.0
4	31.871347972301987	33.0	33.0	33.0	33.0	33.0
5	31.979406586526	33.0	33.0	33.0	33.0	33.0
6	34.880223253809824	37.0	37.0	37.0	27.0	37.0
7	35.054958409215615	37.0	37.0	37.0	33.0	37.0
8	35.21950205513346	37.0	37.0	37.0	33.0	37.0
9	35.306421913344394	37.0	37.0	37.0	33.0	37.0
10	35.32240516257604	37.0	37.0	37.0	33.0	37.0
11	35.34567647722185	37.0	37.0	37.0	33.0	37.0
12	35.31440343902431	37.0	37.0	37.0	33.0	37.0
13	35.32340832354298	37.0	37.0	37.0	33.0	37.0
14	35.29573555599691	37.0	37.0	37.0	33.0	37.0
15	35.33921315824023	37.0	37.0	37.0	33.0	37.0
16	35.33961711567658	37.0	37.0	37.0	33.0	37.0
17	35.30110818990039	37.0	37.0	37.0	33.0	37.0
18	35.30770111189285	37.0	37.0	37.0	33.0	37.0
19	35.30538677241375	37.0	37.0	37.0	33.0	37.0
20	35.30793507057473	37.0	37.0	37.0	33.0	37.0
21	35.32271991274519	37.0	37.0	37.0	33.0	37.0
22	35.31764519745103	37.0	37.0	37.0	33.0	37.0
23	35.30308253187056	37.0	37.0	37.0	33.0	37.0
24	35.311705339980676	37.0	37.0	37.0	33.0	37.0
25	35.29482496860914	37.0	37.0	37.0	33.0	37.0
26	35.257711379144354	37.0	37.0	37.0	33.0	37.0
27	35.20212346959042	37.0	37.0	37.0	33.0	37.0
28	35.18422647200406	37.0	37.0	37.0	33.0	37.0
29	35.223967467961124	37.0	37.0	37.0	33.0	37.0
30	35.17771097518691	37.0	37.0	37.0	33.0	37.0
31	35.17483782792087	37.0	37.0	37.0	33.0	37.0
32	35.19505589761025	37.0	37.0	37.0	33.0	37.0
33	35.17490852047223	37.0	37.0	37.0	33.0	37.0
34	35.1108055247912	37.0	37.0	37.0	33.0	37.0
35	35.116848054776625	37.0	37.0	37.0	33.0	37.0
36	35.0840012657333	37.0	37.0	37.0	33.0	37.0
37	35.113500257522865	37.0	37.0	37.0	33.0	37.0
38	34.97087803515103	37.0	37.0	37.0	27.0	37.0
39	35.04017356704515	37.0	37.0	37.0	27.0	37.0
40	35.07100730153066	37.0	37.0	37.0	33.0	37.0
41	35.083370082239	37.0	37.0	37.0	33.0	37.0
42	34.99365786824928	37.0	37.0	37.0	27.0	37.0
43	34.78500207028186	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	18.0
17	34.0
18	28.0
19	21.0
20	36.0
21	102.0
22	301.0
23	750.0
24	1492.0
25	2771.0
26	4374.0
27	6864.0
28	9645.0
29	13501.0
30	17630.0
31	22445.0
32	28897.0
33	37837.0
34	54175.0
35	99958.0
36	293232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72223886676474	19.742241492488073	13.393377117831019	25.142142522916167
2	16.099050363393378	22.89142634004464	37.82960402072302	23.17991927583897
3	18.120520701135455	26.61103275084915	30.118561507569154	25.14988504044624
4	12.705807898041144	17.711345481231128	38.82232942055672	30.760517200171005
5	14.300598193636995	36.56706871652624	34.82416069426818	14.308172395568587
6	29.77839568304153	39.585472344064016	17.095647021992118	13.54048495090234
7	26.864011095364255	32.08465601341139	23.070682452425594	17.980650438798765
8	24.831263612524026	35.3291074897075	20.3936228586048	19.446006039163674
9	26.473855538088138	14.913435287701851	19.733657396965608	38.879051777244406
10	15.292313699879823	27.67091607447629	33.51264555091378	23.524124674730107
11	34.59693463632048	23.337294360417555	23.71583614139857	18.34993486186339
12	23.467065686845462	25.37828930758329	29.73917814859574	21.415466856975502
13	28.688720498483477	21.919403758823947	25.769118127253325	23.62275761543925
14	22.497399524003487	20.538710904494366	27.563194091449233	29.400695480052917
15	24.45760298389893	28.01646799815526	24.262188574063913	23.263740443881897
16	23.062266672501607	27.875251211030733	25.093667630553995	23.96881448591367
17	22.795654764509646	26.82378366732759	26.060135797024852	24.320425771137916
18	23.94710177370978	26.192768488626918	27.127929953780537	22.732199783882773
19	25.413803898862525	25.701960203459894	26.941436270664948	21.942799627012633
20	24.886134497628433	25.649277421135725	26.78338792369244	22.681200157543397
21	23.6473316928173	26.502469189829696	26.931673965953124	22.91852515139988
22	23.429699623982952	26.258411572034028	26.898684108651082	23.413204695331935
23	23.294205567206735	26.237372122224055	27.400769538916247	23.06765277165296
24	23.49248134221591	26.543706511457245	26.22811476430767	23.73569738201918
25	23.56654020554701	26.276253025472883	27.41608625837791	22.7411205106022
26	23.649351479999055	26.473518906891176	26.930159125566806	22.946970487542963
27	23.599025116053603	26.276926287866804	26.787090866858996	23.336957729220597
28	23.109058408878987	26.28601533018471	27.232790571633437	23.37213568930287
29	24.071150369789372	26.320520027873062	26.422014333756366	23.1863152685812
30	23.78922174233575	26.14934306421913	27.13331605293189	22.92811914051323
31	23.810766138941162	26.009304486283963	26.826140085706303	23.353789289068576
32	22.62969558440859	26.0247895213441	27.35330454014495	23.992210354102355
33	22.706447497315366	26.30755972679012	27.58356027886528	23.40243249702923
34	23.381561362817738	25.798573356987287	27.247602344299654	23.57226293589532
35	23.167295605952987	26.548082717017717	27.082484742191	23.2021369348383
36	23.77171692009385	26.25050073890548	26.83876375559229	23.139018585408383
37	23.496689232177904	25.948542555232763	26.952881731361572	23.60188648122776
38	23.398392922665714	26.29746079088133	26.685428245377214	23.61871804107574
39	23.055870679759376	26.013344060647476	27.220840164141368	23.709945095451776
40	23.039880697903797	26.42942022008948	27.49755100804212	23.033148073964608
41	23.454778648156438	25.733771851572573	27.14055362366652	23.67089587660447
42	22.90893116228653	26.44810325152073	27.310552378131092	23.332413208061645
43	23.41707595409697	25.622010294182	27.16226633587041	23.798647415850617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	21.0
2	30.0
3	136.0
4	242.0
5	242.0
6	314.5
7	387.0
8	390.5
9	394.0
10	505.0
11	616.0
12	616.0
13	962.5
14	1309.0
15	2307.5
16	3306.0
17	3690.0
18	4074.0
19	4074.0
20	3536.5
21	2999.0
22	3188.5
23	3378.0
24	4591.0
25	5804.0
26	5804.0
27	7559.0
28	9314.0
29	11849.0
30	14384.0
31	17588.0
32	20792.0
33	20792.0
34	24488.5
35	28185.0
36	30865.0
37	33545.0
38	36688.5
39	39832.0
40	39832.0
41	42224.0
42	44616.0
43	45879.5
44	47143.0
45	47496.0
46	47849.0
47	47849.0
48	47812.5
49	47776.0
50	47454.0
51	47132.0
52	45464.0
53	43796.0
54	43796.0
55	41109.0
56	38422.0
57	34118.0
58	29814.0
59	27509.5
60	25205.0
61	25205.0
62	21854.0
63	18503.0
64	15226.0
65	11949.0
66	10313.0
67	8677.0
68	8677.0
69	7195.5
70	5714.0
71	4672.0
72	3630.0
73	3031.0
74	2432.0
75	2432.0
76	1822.0
77	1212.0
78	942.0
79	672.0
80	545.0
81	418.0
82	418.0
83	329.5
84	241.0
85	213.5
86	186.0
87	138.0
88	90.0
89	90.0
90	64.5
91	39.0
92	21.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	594122.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.77219022741977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3993757012982	55.736496129809176
2	7.396201703049135	9.43343963934431
3	1.96097372872557	3.7516676897777907
4	0.8754069463860389	2.233064732453405
5	0.5186162637392092	1.6536647513105276
6	0.3162393645751132	1.2100366149049484
7	0.2277460091328342	1.0166703272568345
8	0.16905090526920552	0.8624597191156235
9	0.12891262487915367	0.7398936392858454
>10	0.8450052811992027	10.562171613163082
>50	0.10008456109224927	4.447003982292583
>100	0.060263100929706805	6.987688083965224
>500	0.0015928572932956203	0.6318275895079875
>1k	5.309524310985401E-4	0.7339154878127345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2502	0.4211256273963933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1833	0.30852249201342485	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.1570384533816287	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	652	0.10974177020881233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.732623939190941E-4	0.0	0.0
2	0.0	0.0	0.0011782091893584145	0.0	0.0
3	0.0	0.0	0.0015148403863179617	0.0	0.0
4	0.0	0.0	0.001683155984797735	1.6831559847977351E-4	0.0
5	0.0	0.0	0.0026930495756763762	1.6831559847977351E-4	0.0
6	0.0	0.0	0.004712836757433658	1.6831559847977351E-4	0.0
7	0.0	0.0	0.010772198302705505	1.6831559847977351E-4	0.0
8	1.6831559847977351E-4	0.0	0.011782091893584146	1.6831559847977351E-4	0.0
9	1.6831559847977351E-4	0.0	0.014138510272300975	0.0013465247878381881	0.0
10	0.0010098935908786411	0.0	0.017504822241896447	0.0013465247878381881	0.0
11	0.0010098935908786411	0.0	0.02693049575676376	0.0015148403863179617	0.0
12	0.0010098935908786411	0.0	0.029286914135480593	0.0015148403863179617	0.0
13	0.0011782091893584145	0.0	0.0311383857187581	0.0015148403863179617	0.0
14	0.0011782091893584145	0.0	0.033158172900515384	0.0015148403863179617	0.0
15	0.0011782091893584145	0.0	0.03635616927163108	0.0015148403863179617	0.0
16	0.0011782091893584145	0.0	0.03904921884730746	0.0015148403863179617	0.0
17	0.0011782091893584145	0.0	0.039554165642746775	0.0015148403863179617	0.0
18	0.0013465247878381881	0.0	0.041069006029064735	0.0015148403863179617	0.0
19	0.0013465247878381881	0.0	0.04207889961994338	0.0015148403863179617	0.0
20	0.0013465247878381881	0.0	0.0425838464153827	0.0015148403863179617	0.0
21	0.0013465247878381881	0.0	0.044771949195619755	0.0020197871817572823	0.0
22	0.0013465247878381881	0.0	0.04527689599105907	0.002356418378716829	0.0
23	0.0013465247878381881	0.0	0.04527689599105907	0.0030296807726359234	0.0
24	0.0013465247878381881	0.0	0.04527689599105907	0.0037029431665550174	0.0
25	0.0013465247878381881	0.0	0.04544521158953885	0.003871258765034791	0.0
26	0.0013465247878381881	0.0	0.04561352718801862	0.004039574363514565	0.0
27	0.0013465247878381881	0.0	0.04561352718801862	0.005554414749832526	0.0
28	0.0013465247878381881	0.0	0.04561352718801862	0.015821666257098712	0.0
29	0.0013465247878381881	0.0	0.04561352718801862	0.03719774726402995	0.0
30	0.0013465247878381881	0.0	0.04561352718801862	0.06816781738430827	0.0
31	0.0013465247878381881	0.0	0.04595015838497817	0.14862267345764002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	20	0.0018412207	37.0	10
GGTATCA	670	0.0	24.022387	1
GTATCAA	1625	0.0	21.972307	1
TCACGGC	45	0.0038236654	20.555555	33
CTAGACC	45	0.0038236654	20.555555	3
TTAAGCA	45	0.0038236654	20.555555	4
TTTAGGC	100	2.8691102E-7	18.5	3
CGAGTCG	150	1.2732926E-11	18.5	21
ATTCACG	50	0.0070309793	18.5	31
CGGGATA	50	0.0070309793	18.5	16
GTCCGGC	170	0.0	18.5	15
ACGTCGC	50	0.0070309793	18.5	16
TGTCCGG	180	0.0	18.5	14
CCGTCCG	135	1.1477823E-9	17.814816	28
TTAGGCC	95	3.6004549E-6	17.526316	4
GACCCAT	85	2.7195309E-5	17.411764	6
GGCGAGT	170	5.456968E-12	17.411764	19
GCGAGTC	160	3.45608E-11	17.34375	20
TTCGCCG	150	2.5102054E-10	17.266666	24
CGTCCGC	140	1.8626451E-9	17.178572	29
>>END_MODULE