FastQCFastQC Report
Fri 10 Feb 2017
ERR1633052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275870
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5840.21169391379997826No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5810.21060644506470438No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5650.20480661180991044No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5040.18269474752600862No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5000.18124478921231016No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4470.16203284155580527No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4190.1518831333599159No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4060.14717076884039584No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3870.14028346685032805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.13883350853662957No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3570.12940877949758944No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG3410.12360894624279552No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3300.11962156088012468No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3200.11599666509587848No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3180.11527168593902924No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3070.11128430057635844No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA3010.10910936310581071No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC2890.10475948816471527No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC2770.10040961322361983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCA250.005489194329.615
TCCTATA250.005489194329.62
ACATAAG358.852244E-426.4285721
GGTATCA1200.026.2083341
CTATACA551.8958708E-523.5454544
CCGAGTT551.8958708E-523.54545413
TTGCCGA652.6705911E-622.7692310
CGAGTTC603.7130863E-521.58333414
GTATCAA3250.021.0615391
ACGGAAG450.003818551520.5555555
GCCCAAT902.1401756E-618.512
ACCATCG1002.8552859E-718.518
TTATACC701.2149395E-418.54
TAGGGCA500.007021666518.532
TTTGCCG701.2149395E-418.59
CAGTAAG500.007021666518.51
CCTCTAG609.2125766E-418.51
TGCCGAG701.2149395E-418.511
TAACGGC1552.0008883E-1117.90322736
CGTGCAA1054.764679E-717.61904711
Produced by FastQC (version 0.11.5)