##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275870
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708732373944247	33.0	33.0	33.0	27.0	33.0
2	31.231126980099322	33.0	33.0	33.0	27.0	33.0
3	31.65739297495197	33.0	33.0	33.0	27.0	33.0
4	31.8023706818429	33.0	33.0	33.0	27.0	33.0
5	31.892543589371805	33.0	33.0	33.0	33.0	33.0
6	34.79993475187588	37.0	37.0	37.0	27.0	37.0
7	34.973096023489326	37.0	37.0	37.0	27.0	37.0
8	35.13590096784718	37.0	37.0	37.0	33.0	37.0
9	35.22374306738681	37.0	37.0	37.0	33.0	37.0
10	35.23731105230725	37.0	37.0	37.0	33.0	37.0
11	35.27006198571791	37.0	37.0	37.0	33.0	37.0
12	35.24802261934969	37.0	37.0	37.0	33.0	37.0
13	35.24969007141045	37.0	37.0	37.0	33.0	37.0
14	35.22446442164788	37.0	37.0	37.0	33.0	37.0
15	35.256914488708446	37.0	37.0	37.0	33.0	37.0
16	35.260452386993876	37.0	37.0	37.0	33.0	37.0
17	35.20605357595969	37.0	37.0	37.0	33.0	37.0
18	35.22027041722551	37.0	37.0	37.0	33.0	37.0
19	35.228404683365355	37.0	37.0	37.0	33.0	37.0
20	35.23177946134049	37.0	37.0	37.0	33.0	37.0
21	35.242668648276364	37.0	37.0	37.0	33.0	37.0
22	35.23326929350781	37.0	37.0	37.0	33.0	37.0
23	35.21711675789321	37.0	37.0	37.0	33.0	37.0
24	35.22095914742451	37.0	37.0	37.0	33.0	37.0
25	35.22880704679741	37.0	37.0	37.0	33.0	37.0
26	35.193275818320224	37.0	37.0	37.0	33.0	37.0
27	35.130931235726976	37.0	37.0	37.0	33.0	37.0
28	35.11788523579947	37.0	37.0	37.0	33.0	37.0
29	35.151299525138654	37.0	37.0	37.0	33.0	37.0
30	35.07813825352521	37.0	37.0	37.0	33.0	37.0
31	35.08535542103164	37.0	37.0	37.0	33.0	37.0
32	35.10458186827129	37.0	37.0	37.0	33.0	37.0
33	35.09124225178526	37.0	37.0	37.0	33.0	37.0
34	35.045046579910824	37.0	37.0	37.0	27.0	37.0
35	35.04250190307029	37.0	37.0	37.0	27.0	37.0
36	35.025548265487366	37.0	37.0	37.0	27.0	37.0
37	35.050288179214846	37.0	37.0	37.0	27.0	37.0
38	34.905306847428136	37.0	37.0	37.0	27.0	37.0
39	34.98029506651684	37.0	37.0	37.0	27.0	37.0
40	35.01692463841665	37.0	37.0	37.0	27.0	37.0
41	35.0532207199043	37.0	37.0	37.0	27.0	37.0
42	34.952502990539024	37.0	37.0	37.0	27.0	37.0
43	34.756243882988365	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	16.0
17	14.0
18	11.0
19	8.0
20	18.0
21	57.0
22	155.0
23	368.0
24	771.0
25	1421.0
26	2143.0
27	3310.0
28	4870.0
29	6540.0
30	8532.0
31	10927.0
32	13941.0
33	18003.0
34	26039.0
35	46909.0
36	131810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98669663247181	20.369014390836263	13.173958748685976	25.470330228005945
2	16.283756841990794	22.06184072207924	38.333272918403594	23.32112951752637
3	17.71160329140537	26.654221191140753	29.672309421104142	25.961866096349727
4	12.71142204661616	17.336064088157464	39.31996955087541	30.632544314350962
5	13.658244825461269	37.35310109834342	35.78859607786276	13.200057998332548
6	30.668068293036576	39.42690397651067	17.014898321673254	12.890129408779497
7	27.44336100337115	31.464820386413887	23.750679667959545	17.34113894225541
8	24.16536774567731	36.79595461630478	20.330590495523253	18.70808714249465
9	25.462717946859026	15.66063725667887	20.196469351506146	38.68017544495596
10	15.084278826983724	28.183202232935805	34.24294051546018	22.489578424620294
11	34.238953130097514	23.640120346540037	23.683256606372566	18.437669916989886
12	23.33272918403596	25.79403342153913	29.720520535034616	21.152716859390292
13	26.943125385145176	21.978468119041576	26.374016747018526	24.70438974879472
14	21.739949976438176	20.616232283321857	27.389350056185886	30.25446768405408
15	23.846376916663644	28.76572298546417	24.415848044368722	22.97205205350346
16	23.29684271577192	28.345960053648454	24.82727371588067	23.529923514698954
17	22.28368434407511	27.977308152390616	26.119911552542863	23.619095950991408
18	22.43448000869975	27.77467647805126	27.870373726755354	21.920469786493637
19	24.49487077246529	26.457389350056186	26.751005908580126	22.296733968898394
20	23.44836335955341	27.128720049298582	27.423061586979376	21.99985500416863
21	23.151121905245226	27.219704933483165	27.121832747308517	22.5073404139631
22	22.70598470293979	27.216080037698916	26.97647442636024	23.10146083300105
23	22.736796317105885	27.141769674121868	27.668104541994413	22.45332946677783
24	23.386740131221227	27.72211548917969	25.65266248595353	23.238481893645556
25	22.93036575198463	27.135244861710227	27.336064088157464	22.598325298147678
26	23.19969550875412	27.241816797767065	27.45242324283177	22.106064450647047
27	23.114510457824338	27.24870409975713	26.059375792945954	23.577409649472578
28	22.45659187298365	26.844890709392104	27.897197955558777	22.801319462065468
29	24.27193968173415	26.62159712908254	26.754630804364375	22.351832384818938
30	23.3407039547613	26.670895711748287	27.11929532025954	22.869105013230868
31	22.905353971073332	26.9670496973212	27.383187733352663	22.7444085982528
32	22.244535469605246	26.54619929677022	27.743864863885165	23.46540036973937
33	22.51241526806104	26.795229637147933	27.790988509080368	22.90136658571066
34	23.343241381810273	26.56613622358357	27.779388842570775	22.31123355203538
35	23.271105955703774	27.316127161344113	26.718744336100336	22.694022546851777
36	23.892775582701997	27.0420850400551	26.371116830391127	22.694022546851777
37	22.75999565012506	26.637184180954797	27.08522129988763	23.517598869032515
38	23.446913401239712	26.921013520861276	26.763693043824986	22.868380034074022
39	22.261209990212784	27.148294486533516	27.45351071156704	23.136984811686663
40	22.83539348243738	27.345488817196507	27.64454271939682	22.174574980969297
41	22.567151194403163	26.244245477942506	27.606843803240654	23.581759524413673
42	22.07996520100047	27.560445137202304	27.334976619422193	23.02461304237503
43	23.492587088121216	25.630188132091202	27.359988400333492	23.51723637945409
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	8.0
2	9.0
3	43.0
4	77.0
5	77.0
6	98.5
7	120.0
8	96.0
9	72.0
10	95.0
11	118.0
12	118.0
13	227.5
14	337.0
15	581.0
16	825.0
17	989.5
18	1154.0
19	1154.0
20	1254.5
21	1355.0
22	1661.0
23	1967.0
24	2749.5
25	3532.0
26	3532.0
27	4568.5
28	5605.0
29	6826.5
30	8048.0
31	9671.5
32	11295.0
33	11295.0
34	13271.0
35	15247.0
36	17103.0
37	18959.0
38	20532.0
39	22105.0
40	22105.0
41	22646.0
42	23187.0
43	23400.0
44	23613.0
45	22461.0
46	21309.0
47	21309.0
48	19944.5
49	18580.0
50	18612.5
51	18645.0
52	17443.0
53	16241.0
54	16241.0
55	16424.0
56	16607.0
57	14081.0
58	11555.0
59	10726.5
60	9898.0
61	9898.0
62	9194.5
63	8491.0
64	6783.5
65	5076.0
66	4207.5
67	3339.0
68	3339.0
69	2844.0
70	2349.0
71	2110.5
72	1872.0
73	1819.0
74	1766.0
75	1766.0
76	1621.0
77	1476.0
78	1031.0
79	586.0
80	392.0
81	198.0
82	198.0
83	153.5
84	109.0
85	95.0
86	81.0
87	59.5
88	38.0
89	38.0
90	29.0
91	20.0
92	10.0
93	0.0
94	1.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	275870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.80438170337032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07217255670113	57.46999278659596
2	5.30443478165283	6.768923630584219
3	1.4211786096212753	2.7203226743082287
4	0.6801549669549679	1.7358746851615199
5	0.4591735056000768	1.4648640809690974
6	0.32942046381066825	1.2611081408326297
7	0.22106898268818717	0.9873618827949
8	0.1744726352588351	0.8905694893478098
9	0.1430456934671949	0.8214247824303788
>10	1.0094504580673618	12.96269894238715
>50	0.12758762351659966	5.604746681912964
>100	0.05557149824278564	6.503836185789648
>500	0.0022682244180728833	0.8082760368855182
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	584	0.21169391379997826	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	581	0.21060644506470438	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	565	0.20480661180991044	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	504	0.18269474752600862	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	500	0.18124478921231016	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	447	0.16203284155580527	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	419	0.1518831333599159	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	406	0.14717076884039584	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	387	0.14028346685032805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.13883350853662957	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	357	0.12940877949758944	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	341	0.12360894624279552	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	330	0.11962156088012468	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	320	0.11599666509587848	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	318	0.11527168593902924	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	307	0.11128430057635844	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	301	0.10910936310581071	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	289	0.10475948816471527	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	277	0.10040961322361983	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	3.624895784246203E-4	0.0	0.0
6	0.0	0.0	3.624895784246203E-4	0.0	0.0
7	0.0	0.0	0.001087468735273861	0.0	0.0
8	0.0	0.0	0.0018124478921231015	0.0	0.0
9	0.0	0.0	0.0018124478921231015	0.0	0.0
10	0.0	0.0	0.0018124478921231015	0.0	0.0
11	0.0	0.0	0.002537427048972342	0.0	0.0
12	0.0	0.0	0.002537427048972342	0.0	0.0
13	0.0	0.0	0.002537427048972342	0.0	0.0
14	0.0	0.0	0.0028999166273969623	0.0	0.0
15	0.0	0.0	0.0028999166273969623	0.0	0.0
16	0.0	0.0	0.0032624062058215827	0.0	0.0
17	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
18	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
19	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
20	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
21	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
22	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
23	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
24	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
25	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
26	3.624895784246203E-4	0.0	0.0032624062058215827	0.0	0.0
27	3.624895784246203E-4	0.0	0.0032624062058215827	7.249791568492406E-4	0.0
28	3.624895784246203E-4	0.0	0.0032624062058215827	0.0039873853626708235	0.0
29	3.624895784246203E-4	0.0	0.0032624062058215827	0.009424729039040127	0.0
30	3.624895784246203E-4	0.0	0.0032624062058215827	0.019574437234929496	0.0
31	3.624895784246203E-4	0.0	0.0032624062058215827	0.05836082212636387	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	25	0.0054891943	29.6	15
TCCTATA	25	0.0054891943	29.6	2
ACATAAG	35	8.852244E-4	26.428572	1
GGTATCA	120	0.0	26.208334	1
CTATACA	55	1.8958708E-5	23.545454	4
CCGAGTT	55	1.8958708E-5	23.545454	13
TTGCCGA	65	2.6705911E-6	22.76923	10
CGAGTTC	60	3.7130863E-5	21.583334	14
GTATCAA	325	0.0	21.061539	1
ACGGAAG	45	0.0038185515	20.555555	5
GCCCAAT	90	2.1401756E-6	18.5	12
ACCATCG	100	2.8552859E-7	18.5	18
TTATACC	70	1.2149395E-4	18.5	4
TAGGGCA	50	0.0070216665	18.5	32
TTTGCCG	70	1.2149395E-4	18.5	9
CAGTAAG	50	0.0070216665	18.5	1
CCTCTAG	60	9.2125766E-4	18.5	1
TGCCGAG	70	1.2149395E-4	18.5	11
TAACGGC	155	2.0008883E-11	17.903227	36
CGTGCAA	105	4.764679E-7	17.619047	11
>>END_MODULE