FastQCFastQC Report
Fri 10 Feb 2017
ERR1633051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554413
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15900.2867898119272095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.20544251307238465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5590.10082736155176736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCA950.027.26315912
TCGCCAT1000.025.90000213
CGAGTCG509.080006E-625.90000221
GTATCAA11750.025.034041
AGGACCG451.3220053E-424.6666685
CGCTAAT451.3220053E-424.66666832
GGTATCA3850.023.5454541
TAGACCT400.001929933123.1254
GCCGTCC502.6996576E-422.227
ACGTGGG1450.021.68965536
CGAGACA603.7218095E-521.58333223
GGCCGCA1151.3460522E-1020.91304433
AGAGGTC1500.019.7333328
CGTGGGA1600.019.6562537
GCACCGC951.6722151E-719.47368410
CGGCCTT1052.2531822E-819.3809524
TCATACT1052.2531822E-819.380954
TTGGCCG1254.110916E-1019.2431
TGGCCGC1254.110916E-1019.2432
GCCACGT1650.019.06060633
Produced by FastQC (version 0.11.5)